Recombinant Human TAF9 RNA Polymerase II TAF9
- Known as:
- Recombinant Human TAF9 RNA Polymerase II TAF9
- Catalog number:
- enz-065
- Product Quantity:
- 5
- Category:
- -
- Supplier:
- Prospecbio
- Gene target:
- Recombinant Human TAF9 RNA Polymerase
Related genes to: Recombinant Human TAF9 RNA Polymerase II TAF9
- Gene:
- TAF9 NIH gene
- Name:
- TATA-box binding protein associated factor 9
- Previous symbol:
- TAF2G
- Synonyms:
- TAFII31, TAFII32, TAFIID32, MGC5067, CGI-137, MGC1603, MGC3647, AD-004
- Chromosome:
- 5q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1995-07-13
- Date modifiied:
- 2015-11-20
- Gene:
- TAF9B NIH gene
- Name:
- TATA-box binding protein associated factor 9b
- Previous symbol:
- TAF9L
- Synonyms:
- TAFII31L, DN-7, DN7, TFIID-31
- Chromosome:
- Xq21.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-03
- Date modifiied:
- 2016-10-05
Related products to: Recombinant Human TAF9 RNA Polymerase II TAF9
Related articles to: Recombinant Human TAF9 RNA Polymerase II TAF9
- TFIID is a multiprotein complex that plays a pivotal role in the regulation of RNA polymerase II (Pol II) transcription owing to its core promoter recognition and co-activator functions. TAF6 is a core TFIID subunit whose splice variants include the major TAF6alpha isoform that is ubiquitously expressed, and the inducible TAF6delta. In contrast to TAF6alpha, TAF6delta is a pro-apoptotic isoform with a 10 amino acid deletion in its histone fold domain that abolishes its interaction with TAF9. TAF6delta expression can dictate life versus death decisions of human cells. - Source: PubMed
Publication date: 2010/01/22
Wilhelm EmmanuelleKornete MaraTargat BriceVigneault-Edwards JimmyFrontini MattiaTora LaszloBenecke ArndtBell Brendan - TFIID plays a key role in transcription initiation of RNA polymerase II preinitiation complex assembly. TFIID is comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). A second set of transcriptional regulatory multiprotein complexes containing TAFs has been described (called SAGA, TFTC, STAGA, and PCAF/GCN5). Using matrix-assisted laser desorption ionization mass spectrometry, we identified a novel TFTC subunit, human TAF9Like, encoded by a TAF9 paralogue gene. We show that TAF9Like is a subunit of TFIID, and thus, it will be called TAF9b. TFIID and TFTC complexes in which both TAF9 and TAF9b are present exist. In vitro and in vivo experiments indicate that the interactions between TAF9b and TAF6 or TAF9 and TAF6 histone fold pairs are similar. We observed a differential induction of TAF9 and TAF9b during apoptosis that, together with their different ability to stabilize p53, points to distinct requirements for the two proteins in gene regulation. Small interfering RNA (siRNA) knockdown of TAF9 and TAF9b revealed that both genes are essential for cell viability. Gene expression analysis of cells treated with either TAF9 or TAF9b siRNAs indicates that the two proteins regulate different sets of genes with only a small overlap. Taken together, these data demonstrate that TAF9 and TAF9b share some of their functions, but more importantly, they have distinct roles in the transcriptional regulatory process. - Source: PubMed
Frontini MattiaSoutoglou EviArgentini ManuelaBole-Feysot ChristineJost BernardScheer ElisabethTora Làszlò - The majority of the TATA-binding protein (TBP)-associated factors (TAFs) that constitute transcription factor II D (TFIID) contain histone fold motifs (HFMs). Our previous results utilizing DT40 cells containing a conditional TAF9 allele indicated that the histone 3-like TAF9 is essential for cell viability but largely dispensable for general transcription. In this study, we investigated further the role of TAF9 structural domains in TFIID integrity and cell growth and the functions of a TAF9-related factor, TAF9L. We first show that TAF9 depletion severely disrupts TFIID, indicating that the observed ongoing transcription is initiated with at least partially TAF-free TATA-binding protein. We also provide evidence for specific roles of TAF HFMs, highlighting the functional significance of HFM specificity observed in vitro and, importantly, of the TAF9-histone 3 similarity. Although we provide evidence that TAF9 and TAF9L are partly redundant, RNA interference experiments suggest that TAF9L is essential for HeLa cell growth. Strikingly, we provide evidence that TAF9L plays a role in transcriptional repression and/or silencing. - Source: PubMed
Publication date: 2003/07/01
Chen ZhengManley James L - Typical programmed cell death (PCD) requires de novo macromolecular synthesis and shares common morphological changes referred to as apoptosis. To elucidate the molecular mechanism of apoptosis, we isolated cDNA clones that are induced in differentiated PC12 cells deprived of NGF by differential display method. Among such clones, homology searches revealed that the one clone encodes the rat TATA-binding-protein-associated factor TAFII31, a component of TFIID, and a transcriptional coactivator of the p53 protein. Northern analysis of various organs in human showed one band in heart, brain, skeletal muscle and pancreas, whose size is approximately 1.1 kb which identical to that of human TAFII31 mRNA, although the size of rat human TAFII31 mRNA is approximately 2.7 kb. The deduced amino acid sequence of the rat TAFII31 was 77% identical to that of the human TAFII31. Northern analysis of various organs in adult mice showed that expression levels of TAFII31 mRNA were strong in heart but weak in spleen, although this gene is ubiquitously expressed. - Source: PubMed
Aoki TKoike TNakano TShibahara KNishimura HKikuchi HHonjo T