POLR2K antigen

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636.35 €

Known as:: POLR2K antigenic
Catalog number:: 'H00005440-P02-10
Product Quantity:: 10
Category:: -
Supplier:: ACR
Gene target:: POLR2K antigen

Related genes to: POLR2K antigen

Gene:: POLR2K ^{NIH gene}
Name:: RNA polymerase II subunit K
Previous symbol:: -
Synonyms:: RPB10alpha
Chromosome:: 8q22.2
Locus Type:: gene with protein product
Date approved:: 1993-12-07
Date modifiied:: 2016-10-05

Related products to: POLR2K antigen

Related articles to: POLR2K antigen

Multinucleated giant cells in human pancreatic cancer are a distinct macrophage population undergoing a DNA damage response and associated with an aggressive tumor microenvironment.
Macrophages constitute a dominant and functionally diverse immune population within the microenvironment of pancreatic ductal adenocarcinoma (PDAC), yet how macrophage heterogeneity contributes to the tumor remains poorly defined. In an institutional cohort of 145 PDAC specimens, we identified a population of multinucleated giant cells (MGCs) of macrophage origin, an entity previously described in chronic inflammation but rarely in cancer. CD68⁺ MGCs were present in 28% of tumors, enriched in squamous, non-glandular regions, and more frequent after neoadjuvant chemotherapy. By integrating spatial transcriptomics and quantitative imaging, we defined the features of these cells, which, compared with MGCs in non-neoplastic inflammatory lesions, lacked canonical polarization markers (HLA-DR, CD163) and displayed a distinctive transcriptional program characterized by upregulation of the POLR2K, TUBA8, COX5B, and VDAC1 genes, which encode proteins involved in DNA repair, oxidative stress, and MYC signaling. Spatial analyses revealed activation of hypoxia and extracellular matrix-remodeling pathways in MGC-associated niches, and experimental hypoxia promoted MGC formation in vitro. Consistent with these data, we found that in the TCGA PAAD dataset a macrophage MGC gene signature was enriched in the squamous PDAC subtype and correlated with poorer overall survival (p = 0.018). Morphometric and immunofluorescence analyses further showed increased 53BP1⁺Ki67⁺ nuclei and nuclear atypia in MGCs, indicating ongoing proliferation despite DNA damage. Together, these data identify MGCs of macrophage origin as an immune cell state shaped by hypoxia and stress signaling, associated with aggressive tumor phenotypes, and potentially exploitable as an immune classifier in PDAC. - Source: PubMed
Publication date: 2026/06/30
Viatore MarikaPolidori RebeccaPutignano Anna RitaBonometti ArturoRahal DaoudBarbagallo MarialuisaVeghini LisaDonisi GretaBasso GianlucaGiuliano DesirèeMarchini SergioErreni MarcoFumagalli Maria RitaPasqualini FabioGrizzi FabioSpaggiari PaolaUccella SilviaBozzarelli SilviaZerbi AlessandroCapretti Giovanni LuigiCorbo VincenzoLocati MassimoMantovani AlbertoMarchesi Federica
Protective effect of energized structured water on bioenergetic function and oxidative stress in H9c2 cells.
The energized structured water (ESW) concept supposes water can be imbued with energetic frequencies or vibrations, improving its quality and providing various health benefits. While some studies claim benefits, the mechanisms and effects of ESW are not well understood. This study investigates the impact of ESW on cellular bioenergetic function and oxidative stress in H9c2 cells. H9c2 cells were pretreated with ESW or control water (CW) at equivalent dilutions. Mitochondrial function was assessed using the Agilent Seahorse XF Pro Analyzer. Cell viability and reactive oxygen species (ROS) production were evaluated under HO-induced oxidative stress conditions. Differential gene expression analysis and Gene Ontology (GO) enrichment were conducted to identify significantly affected genes and biological processes in ESW-treated H9c2 cells. Results demonstrated that ESW pretreatment significantly increased maximal and spare respiratory capacity in H9c2 cells. In addition, ESW treatment increased the glycolytic ATP production without affecting mitochondrial and total ATP production. ESW treatment enhanced cell viability and reduced ROS production in cells exposed to HO-induced oxidative stress. Based on differential gene expression analysis, 717 genes including Myh1, Akr1b8, Hmox1, Kcng1, Ugt1a6, Clu, Gaa, Ftl1, Hspb7, and Gba1 were up-regulated and 422 genes including Hist1h2an, Slfn4, Rpl22l1, Polr2k, Il1rl1, Ndufb1, Atp5mkl1 were down-regulated by ESW compared to the controls. GO analysis indicated that ESW treatment significantly affects biological processes related to cellular stress response pathways. These findings suggest that ESW treatment may enhance cellular bioenergetics and stress resistance in H9c2 cells, potentially through modulation of gene expression related to stress responses and energy metabolism. - Source: PubMed
Publication date: 2025/08/05
Ranaweera Sachithra SZuniga-Hertz Juan PChitteti RamamurthyChernov Andrei VPatel Hemal H
The lack of homozygotes with a large deletion encompassing and in primary ciliary dyskinesia patients suggests the lethal effect of the loss of POLR2K protein.
- Source: PubMed
Publication date: 2025/01/20
Rabiasz AlicjaDrobna-Śledzińska MonikaKaźmierczak PatrycjaWitt MichałZiętkiewicz Ewa
Integrated analysis of single-cell RNA sequencing and bulk transcriptome data identifies a pyroptosis-associated diagnostic model for Parkinson's disease.
Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by an insidious onset. Despite the emphasis on motor symptom-based diagnosis, there remains an unmet clinical need for effective diagnostic approaches during the prodromal phase of PD. Recent advances in single-cell RNA sequencing (scRNA-seq) and bulk transcriptomic analyses of PD patients open avenues for identifying potential diagnostic biomarkers. A comprehensive cell trajectory analysis was conducted using scRNA-seq datasets to identify gene expressions associated with the cellular transition from healthy to PD-associated states. Integration of scRNA-seq datasets with weighted gene co-expression network analysis (WGCNA) allowed extraction of pyroptosis-associated differentially expressed genes (PDEGs). Using LASSO logistic regression, support vector machine recursive feature elimination (SVM-RFE) and random forest methods, we developed a diagnostic model centred on PDEGs. In addition, immunoinfiltration, inflammatory signalling pathways and intercellular communication were detected by scRNA-seq analyses. In PD patients, the number of cells including metencephalic-like cells, excitatory neurons, inhibitory neurons and MHB-like cells was significantly reduced, whereas the proportion of astrocytes and microglia, immunoinfiltration and inflammatory signalling pathways were upregulated compared to healthy individuals. Using scRNA-seq and WGCNA analyses, two pyroptosis-related diagnostic genes, POLR2K and TIMM8B, were identified and a diagnostic model based on them was constructed, which showed promising performance upon validation. This study established a pyroptosis-related diagnostic model for PD through the analyses of scRNA-seq combined with bulk transcriptome data, which improved the understanding of the role of PDEGs in PD and provided new insights into the diagnostic strategies for this neurodegenerative disease. - Source: PubMed
Publication date: 2024/11/18
Wang LinQin YidanSong JiaXu JingQuan WeiSu HangZeng HuibinZhang JianLi JiaChen Jiajun
Bioinformatic validation and machine learning-based exploration of purine metabolism-related gene signatures in the context of immunotherapeutic strategies for nonspecific orbital inflammation.
Nonspecific orbital inflammation (NSOI) represents a perplexing and persistent proliferative inflammatory disorder of idiopathic nature, characterized by a heterogeneous lymphoid infiltration within the orbital region. This condition, marked by the aberrant metabolic activities of its cellular constituents, starkly contrasts with the metabolic equilibrium found in healthy cells. Among the myriad pathways integral to cellular metabolism, purine metabolism emerges as a critical player, providing the building blocks for nucleic acid synthesis, such as DNA and RNA. Despite its significance, the contribution of Purine Metabolism Genes (PMGs) to the pathophysiological landscape of NSOI remains a mystery, highlighting a critical gap in our understanding of the disease's molecular underpinnings. - Source: PubMed
Publication date: 2024/03/28
Wu ZixuanFang ChiHu YiPeng XinZhang ZheyuanYao XiaoleiPeng Qinghua

POLR2K antigen

Related genes to: POLR2K antigen

Related products to: POLR2K antigen

Related articles to: POLR2K antigen

Multinucleated giant cells in human pancreatic cancer are a distinct macrophage population undergoing a DNA damage response and associated with an aggressive tumor microenvironment.

Protective effect of energized structured water on bioenergetic function and oxidative stress in H9c2 cells.

The lack of homozygotes with a large deletion encompassing and in primary ciliary dyskinesia patients suggests the lethal effect of the loss of POLR2K protein.

Integrated analysis of single-cell RNA sequencing and bulk transcriptome data identifies a pyroptosis-associated diagnostic model for Parkinson's disease.

Bioinformatic validation and machine learning-based exploration of purine metabolism-related gene signatures in the context of immunotherapeutic strategies for nonspecific orbital inflammation.