IRF7 Reverse PCR Primer (450bp position)
- Known as:
- IRF7 Reverse PCR test kit Primer (450bp position)
- Catalog number:
- MP1154
- Product Quantity:
- ea
- Category:
- -
- Supplier:
- Panomics
- Gene target:
- IRF7 Reverse PCR Primer (450bp position)
Related genes to: IRF7 Reverse PCR Primer (450bp position)
- Gene:
- IRF7 NIH gene
- Name:
- interferon regulatory factor 7
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 11p15.5
- Locus Type:
- gene with protein product
- Date approved:
- 1996-11-13
- Date modifiied:
- 2019-04-23
Related products to: IRF7 Reverse PCR Primer (450bp position)
Related articles to: IRF7 Reverse PCR Primer (450bp position)
- Hope has been associated with improved quality of life and lower mortality in cancer, but the underlying biological mechanisms are poorly characterized. We previously reported that hope was associated with less inflammation and more normalized diurnal cortisol pre-treatment among women with ovarian cancer. We also reported associations of socio-environmental factors with pro-metastatic processes. Here, we used genome-wide transcriptional profiling to quantify associations between hope and tumor molecular signatures reflecting invasiveness, inflammation, and cellular immunity. - Source: PubMed
Publication date: 2026/09/08
Lutgendorf Susan KCorn Benjamin WThaker Premal HGoodheart Michael JPenedo Frank JSood Anil KCole Steven W - Asthma and chronic obstructive pulmonary disease (COPD) are chronic respiratory diseases associated with inflammation and structural changes in the airways. Hypoxia is a key element of the pulmonary microenvironment, particularly in obstructive diseases, where it can be transient (e.g., during asthma exacerbations) or chronic (COPD). Pulmonary vascular endothelial cells (ECs) are important components of the immune response and (like the epithelium) are exposed to hypoxia and viral infections. Although it has been demonstrated that ECs can be infected by respiratory viruses and that hypoxia can affect their function, the impact of hypoxia on the antiviral capabilities of the endothelium remains poorly understood. This study examined the influence of acute and chronic hypoxia on the antiviral properties of human lung microvascular ECs. Acute and chronic hypoxia upregulated endosomal RNA receptors TLR3 and TLR7, whereas they downregulated cytosolic RIG-I and MDA5. Acute hypoxia downregulated mRNA and protein expression of IRF3 and IRF7 and inhibited effector antiviral mechanisms, whereas chronic hypoxia induced IRF7, OAS-1, protein kinase-R (PKR), and Mx1 at both mRNA and protein levels by flow cytometry. In addition, both hypoxia models caused increased secretion of lymphocyte-recruiting chemokines, whereas the secretion of cytokines/chemokines responsible for the involvement of monocytes and macrophages was inhibited. Acute hypoxia suppresses antiviral regulatory and effector pathways, whereas chronic hypoxia enhances viral RNA detection and intracellular antiviral mechanisms. These findings highlight the complex role of hypoxia in shaping endothelial immune responses and suggest that, depending on its duration, it may influence susceptibility to viral infections in chronic respiratory diseases. - Source: PubMed
Szewczyk RobertKalinowska JolantaSochacka EwelinaChałubiński MaciejWardzyńska Aleksandra - Type I interferons (IFNs) serve as pivotal functional molecules in the antiviral immune response of fish, and their signaling pathways require precise negative regulation to avoid tissue damage caused by excessive immune activation. In the present study, DEAD-box RNA helicase 3b (DDX3b), an ATP-dependent RNA helicase involved in cellular RNA metabolism and host-pathogen interaction, was identified in black carp (Mylopharyngodon piceus). Sequence and structural analyses showed that bcDDX3b was evolutionarily conserved and contained the characteristic DEXDc and HELICc domains of DEAD-box RNA helicases. Subcellular localization analysis showed that bcDDX3b was predominantly distributed in the cytoplasm and formed distinct cytoplasmic aggregates. Functional assays verified that black carp DDX3b (bcDDX3b) acted as a negative regulator of type I IFN signaling. Overexpression of bcDDX3b markedly suppressed both basal and virus-induced IFN promoter activities and decreased the expression levels of interferon-stimulated genes (ISGs), thereby blocking the establishment of cellular antiviral status. Mechanistically, bcDDX3b directly interacted with bcIRF7, the core transcription factor of IFN signaling pathway, and the DNA-binding domain (DBD) of bcIRF7 was indispensable for the interaction. The binding of bcDDX3b to bcIRF7 significantly impaired bcIRF7-mediated antiviral defenses against spring viraemia of carp virus (SVCV) and grass carp reovirus (GCRV). Further mechanistic investigations revealed that bcDDX3b inhibited the phosphorylation and ubiquitination of bcIRF7 and facilitated bcIRF7 degradation via a lysosome-dependent pathway. Collectively, bcDDX3b functions as a critical negative modulator of RLR-triggered type I IFN signaling in black carp. This study provides novel insights into the regulatory mechanism of IRF7 and the immune homeostasis underlying antiviral responses in teleost fish. - Source: PubMed
Publication date: 2026/09/02
Chen LiangZhao ZitingDu ZhihaoLiang XinyiDeng ShuhaoWu SitongYan JunWu HuiLiu JiXiao JunFeng Hao - Infections are frequent in critically ill patients with earthquake-associated crush injury (CI) and contribute to poor outcomes, yet the immune pathways underlying posttrauma susceptibility remain poorly defined. We investigated longitudinal changes in damage-associated molecular pattern (DAMP)/PAMP sensing and downstream inflammatory programs in CI patients requiring intensive care. - Source: PubMed
Yuksel Recep CivanDemir Busra SenizHouran Mohammad AhmadAsan MineTemel SahinKaynar Ahmet SafaUlger BirkanTalih TutkunEsmaoglu AliyeSungur MuratGündoğan KürşatEken Ahmet - Newcastle disease virus (NDV) is an important avian pathogen that can circulate in migratory birds, including . This study examined the transcriptional responses and anti-NDV activities associated with recombinant IFN-α and Mx proteins from . The IFN-α and Mx open reading frames were cloned (GenBank: OP263971 and OP263970), expressed using a pET32a(+)/ system, and assessed using chicken embryos, DF-1 cells, and primary gull lymphocytes. qRT-PCR analysis showed that NDV infection increased IFN-α and Mx transcript abundance in gull lymphocytes, with peak levels at 48 h post-infection ( < 0.01). In chicken embryos, recombinant IFN-α was associated with higher embryo survival and lower NDV NP transcript abundance than recombinant Mx under the tested conditions. In DF-1 cells and primary gull lymphocytes, 0.25 mg/mL recombinant IFN-α was associated with reduced NDV NP transcript abundance, and morphological inspection suggested less severe cytopathic effects in DF-1 cells. IFN-α treatment was also accompanied by lower transcription of TLR7, MyD88, IRF7, and Mx compared with the NDV group at 48 h. These data support a role for gull IFN-α in limiting NDV-associated transcriptional responses in vitro, while further protein-level and infectious-virus assays are required to define the underlying mechanism. - Source: PubMed
Publication date: 2026/08/03
Chang HuaPu ShaoxiaYuan MingxiuChen YiRen ShengjieZhang HongliDuan GangDai FeiyanXiang Xun