CREB2 TAD in vivo Kinase Assay Kit
- Known as:
- CREB2 TAD vivo Kinase Assay Kit
- Catalog number:
- LK0006
- Product Quantity:
- 10ug
- Category:
- Peptides
- Supplier:
- Panomics
- Gene target:
- CREB2 TAD vivo Kinase Assay Kit
Related genes to: CREB2 TAD in vivo Kinase Assay Kit
- Gene:
- ATF2 NIH gene
- Name:
- activating transcription factor 2
- Previous symbol:
- CREB2
- Synonyms:
- TREB7, CRE-BP1, HB16
- Chromosome:
- 2q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1991-08-01
- Date modifiied:
- 2016-10-05
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- Gestational diabetes mellitus (GDM) is a common metabolic disorder during pregnancy. Metformin (MET) has emerged as a promising alternative to insulin, but its mechanism of action in the placenta remains incompletely understood. In this study, we aimed to examine whether MET counteracts high-glucose-induced ferroptosis by regulating activating transcription factor 2 (ATF2) and to delineate the underlying molecular mechanism in GDM. We established in vitro GDM cell models using HTR-8/SVneo trophoblast cells and an in vivo STZ-induced hyperglycemic rat model, followed by treatments including ATF2 lentiviral manipulation and MET administration. We evaluated cell viability, ferroptosis markers (ROS, MDA, GSH, iron deposition), and PI3K/Akt signaling pathway activity. Our results demonstrated that in GDM patients and high-glucose-challenged HTR-8/SVneo cells, MET promoted cell viability by suppressing ATF2, reactivating PI3K/Akt signaling, enhancing nuclear Nrf2 translocation, and upregulating GPX4, while concurrently lowering ROS and MDA levels, elevating GSH, and reducing iron deposition. These protective effects were reversed by ATF2 overexpression or pathway inhibitors. In STZ-induced hyperglycemic rats, MET decreased placental ATF2 expression, restored the PI3K/Akt-Nrf2-GPX4 axis, alleviated ferroptosis, and reduced fetal weight. Collectively, our findings indicate that MET suppresses ATF2, activates PI3K/Akt, drives Nrf2 nuclear import, upregulates GPX4, and curbs high-glucose-induced trophoblast ferroptosis, offering new mechanistic insight and therapeutic avenues for GDM. - Source: PubMed
Xia DandanZhang YuhuiLiu WenjieLi SiyuZhang ChenyingShe GuangtongWang Huiyan - Anxiety is common among peri- and postmenopausal women, and many conventional treatments cause side effects. This multicenter, double-blind, randomized, placebo-controlled trial was conducted from December 2021 to June 2023 at 18 centers in China, enrolling 360 women aged 40‒65 years with menopausal anxiety. Participants were randomly assigned to receive Xiangshao granules (high-dose group, = 180) or a placebo (very-low-dose group, = 180) for 8 weeks. The primary outcome was the reductive ratio in self-rating anxiety scale (SAS) scores after 8 weeks, while secondary outcomes included the self-rating depression scale (SDS), Kupperman Index, and gastrointestinal symptom score (GIS). After eight weeks, the mean SAS reductive ratio was greater in the high-dose group than in the very-low-dose group (37.7% . 28.9%, = 0.0052), and scores declined more in the high-dose than in the very-low-dose group, with a higher clinical improvement rate (69.8% 56.9%, = 0.0151). Although no significant between-group differences were observed for SDS, Kupperman Index, or GIS, all showed improvements from baseline. Adverse event rates were comparable. Transcriptomic analysis revealed modifications in the dopaminergic synapse pathway following Xiangshao treatment, while network pharmacology identified FOS, ATF2, and CREB1 as key dopaminergic pathway-related targets. Metabolomic data corroborated these findings. Collectively, Xiangshao granules safely and effectively alleviate menopausal anxiety, potentially by modulating dopaminergic signaling. - Source: PubMed
Publication date: 2026/06/16
Tang RuiyiXie ZhuolinFan YuboZhang JingLu LingZhuang YaliGuo CunjuShu KuanyongLi ShizeZhang PingLiu KunZhang YuanyuanJi JianghaiRen QinglingMeng QingfangLin YiHuang XianghuaLi HuiminLiu YingDeng QijianChen HongyanSu MingmingZhang XinleiWang YufeiLiang YaoyaoZhang PengChen Rong - Acute myocardial infarction (AMI) is governed by intricate molecular networks. The precise role of tumor necrosis factor receptor-associated factor 1 (TRAF1) in AMI pathogenesis remains incompletely understood. This study aimed to delineate the function, regulation, and therapeutic potential of TRAF1 in AMI. - Source: PubMed
Publication date: 2026/09/01
Zhang FeiXin HongXu QuanchengLi Chengliang - Esculetin, a coumarin derivative, exhibits diverse biological activities; however, its impact on pancreatic β-cell stress responses and survival remains poorly defined. In this study, we investigated the effects of esculetin on cellular stress signaling, apoptosis, and functional gene expression in INS-1 pancreatic β-cells. Following treatment with 3000 µM esculetin for 6 h, cell viability, proliferation, apoptosis, oxidative stress, endoplasmic reticulum (ER) stress markers, MAPK signaling components, cell cycle distribution, and β-cell-specific gene expression were assessed. In silico molecular docking was performed to explore putative interactions between esculetin and proteins involved in ER stress, MAPK signaling, and apoptosis. Treatment with 3000 µM esculetin for 6 h increased apoptotic cell death by approximately 32-fold and elevated total oxidation status and oxidative stress index. Intracellular insulin, Ca²⁺, and CA19-9 levels were increased, accompanied by cell cycle arrest characterized by G0/G1 phase accumulation and a reduction in the G2/M population. Expression of ER stress sensors ATF6, IRE1α, and CHOP was upregulated, whereas PERK expression was reduced. In parallel, MAPK/JNK pathway activation was evidenced by increased levels of p-ATF2, p-c-Jun, and HSP90, along with decreased p-Elk1. Gene expression analysis revealed marked downregulation of β-cell functional and identity-associated genes, including Ins1, Ins2, IR, Akt, MafA, Nkx6.1, Pdx1, NeuroD1, and Pax6, while FoxO1 expression was upregulated. Collectively, these findings suggest the involvement of ER stress-associated MAPK/JNK signaling in esculetin-induced apoptosis and functional impairment in INS-1 pancreatic β-cells, providing mechanistic insight into stress-associated β-cell dysfunction. - Source: PubMed
Publication date: 2026/08/29
Karatug Kacar Ayse - Adrenocorticotropic hormone (ACTH) is an effective treatment for infantile epileptic spasms syndrome (IESS); however, its mechanism of action remains incompletely understood. This study aimed to evaluate ACTH treatment response at the level of protein-protein interactions (PPIs) in patients with confirmed and presumed monogenic developmental and epileptic encephalopathies (DEEs). - Source: PubMed
Publication date: 2026/08/19
Kayar Çisem DumanAkbaş SinanKürekçi FulyaGüçlü Burcu YamanKılıç Mehmet AkifGenç Hülya MaraşYıldız Edibe Pembegül