EEF2 (C-term) antibody Host Rabbit
- Known as:
- EEF2 (C-terminus) (anti-) Host Rabbit
- Catalog number:
- 'AP17308PU-N
- Product Quantity:
- 0.1 mg
- Category:
- -
- Supplier:
- ACR
- Gene target:
- EEF2 (C-term) antibody Host Rabbit
Related genes to: EEF2 (C-term) antibody Host Rabbit
- Gene:
- EEF2 NIH gene
- Name:
- eukaryotic translation elongation factor 2
- Previous symbol:
- EF2
- Synonyms:
- EEF-2
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-03-11
- Date modifiied:
- 2015-09-11
Related products to: EEF2 (C-term) antibody Host Rabbit
Related articles to: EEF2 (C-term) antibody Host Rabbit
- Digital PCR (dPCR) enables absolute, precise nucleic acid quantification without the need for standard curves. Still, technical variability across the sample life cycle remains a challenge in complex multi-organ preclinical studies. This study evaluated the necessity of internal reference genes (i.e., housekeeping genes; HKGs) for normalizing reverse transcription (RT)-dPCR data from recombinant adeno-associated virus (rAAV) studies in mice and non-human primates (NHPs). HKGs were ranked using statistical analysis and further evaluated with the BestKeeper and geNorm algorithms. In mice, , , and were identified as the most stable HKGs. Normalization to a single HKG () consistently and markedly reduced transgene-expression variability across tissues. In NHPs, and demonstrated the highest stability. Importantly, multiplexing HKGs allowed to differentiate technical from biological variation, thereby mitigating common assay pitfalls. Collectively, these findings underscore that normalization is essential for reliable inter- and intra-group comparability in RT-dPCR. We strongly recommend multiplexing at least two medium-expression, validated HKGs to derive a robust normalization factor, thereby significantly improving accuracy in complex preclinical settings. - Source: PubMed
Publication date: 2026/04/16
Mackeben KlausMüller SarahDolim KimberlyOtteneder Michael BRos FrancescaFakhiri Julia - In June of 2022, 3 creole wrasses, Bodianus parrae, from a public display aquarium were submitted for diagnostic examination at Mississippi State University's College of Veterinary Medicine. Fresh myxospores recovered from the intestines were morphologically consistent with previous accounts of Enteromyxum leei (Diamant, Lom & Dyková, 1994). Molecularly, myxospores from the present study were 99.7-100% and 99.7-99.8% similar to publicly available E. leei sequences at 18S small subunit ribosomal (18S) and 28S large subunit ribosomal (28S) genes, respectively. Internal transcribed spacer region 1 (ITS1), 5.8S ribosomal rRNA gene (5.8S), and internal transcribed spacer region 2 (ITS2) sequences were 95.8-98.0%, 100%, and 99.9% similar, respectively, to the only publicly available data from these regions from E. leei. Eukaryotic elongation factor 2 (EEF2) sequences were 98.8% similar to the available EEF2 sequence of E. leei in GenBank. Lastly, cytochrome c oxidase subunit 1 (COI) sequences were 98.6% similar to previously published data. Bayesian phylogenetic analyses using both single locus 18S sequence data, concatenated 18S, 28S, and EEF2 data, and concatenated 18S, 28S, and COI amino acid sequences from each of the 3 wrasses grouped E. leei from the present study with previously published E. leei sequences with high Bayesian and maximum likelihood support. This is the first account of E. leei from B. parrae. Histological assessment reveals pathological changes to intestinal epithelia consistent with previous accounts of E. leei, with 18S, ITS1, 5.8S, ITS2, 28S, EEF2, and COI sequence data providing greater phylogenetic resolution of this enigmatic species. These morphological and molecular data from an enteromyxosis outbreak in a mixed-species exhibit at a public aquarium offer robust species-level identification of the causative agent, E. leei. - Source: PubMed
Publication date: 2026/05/13
Woodyard Ethan TStilwell Justin MPerry Sean MDelaune Alexa JStilwell Natalie KRosser T GrahamNguyen Jonah AGriffin Matt J - The river buffalo is an economically important livestock species supplying milk and meat. However, a multi-tissue transcriptomic atlas for the key dairy river buffalo breeds, Murrah and Nili-Ravi, has not yet been established, and the lack of stable reference genes has hindered in-depth studies of their biological functions and the molecular mechanisms underlying key economic traits such as lactation. We established a multi-tissue gene expression atlas across 20 tissues and identified 717 housekeeping genes (HKGs), and and were further shown to be stable candidate reference genes under the conditions tested. We found 8368 tissue-specific genes (TSGs), predominantly enriched in the reproductive system. Exploratory analysis of mammary tissue (dry-period vs. public lactating samples, confounded by batch effects) revealed mammary-enriched hub genes including ; these findings are preliminary and require validation. Dynamic analysis across lactation stages (early, peak, mid-, late) identified candidate genes including and . Phenotypic data showed strong negative correlations between milk yield and protein/fat content, and a positive correlation with lactose content. However, causal or regulatory roles were not inferred due to lack of paired individual-level data. Cross-dataset comparisons are descriptive only, and are not key conclusions. In summary, this study lays the foundation for advancing research in lactation trait genetics and functional genomics in river buffalo, with novel reference genes and lactation stage-specific transcriptional dynamics as its main contributions. - Source: PubMed
Publication date: 2026/04/30
Song XinhuiWang DongLuo XierQin ChaobinLi LingYang YanyanPi YifeiDeng YanfeiCui KuiqingLi ZhipengXu WeiLiu Qingyou - Recently, a novel circular RNA (circRNA), circPTP4A2 (hsa_circ_0007364), was reported to promote cervical cancer progression. However, its specific role in non-small cell lung cancer (NSCLC) remains unclear. Here, we observed that circPTP4A2 is significantly upregulated in NSCLC tissues and cell lines. Furthermore, multivariate Cox proportional hazards regression analysis confirmed that high circPTP4A2 expression is independently associated with poor prognosis in NSCLC patients after adjusting for confounding clinicopathological covariates. Knockdown of circPTP4A2 significantly suppressed the proliferation, migration, and invasion capabilities of H1299 and A549 cells. Additionally, we identified miR-183-5p as a direct targeted of circPTP4A2. In NSCLC clinical samples, circPTP4A2 expression showed a negative correlation with miR-183-5p levels. Moreover, we demonstrated that circPTP4A2 enhances the expression of EEF2, a downstream target of miR-183-5p. Collectively, our findings elucidate that circPTP4A2 promotes NSCLC progression by sponging miR-183-5p to upregulate EEF2, suggesting it could be a promising therapeutic target for NSCLC. - Source: PubMed
Publication date: 2026/05/08
Shen QiupingJin HaoZhu Xiaofeng - Eukaryotic elongation factor 2 kinase (eEF2K) phosphorylates eukaryotic elongation factor 2 (eEF2) and slows translation elongation. In the nervous system, this pathway links neuronal activity, calcium signaling, energy status, and stress responses to selective protein synthesis programs that shape synaptic plasticity, circuit excitability, and cell survival. Dysregulated eEF2K/eEF2 signaling has been implicated in epilepsy, Alzheimer's disease, Parkinson's disease, major depressive disorder, Down syndrome, and other brain conditions. However, the literature remains fragmented, largely preclinical, and often interpreted in an overly therapeutic manner. This review synthesizes the field using a mechanistic framework. Across disorders, altered eEF2 phosphorylation converges on five major axes: synaptic plasticity and excitatory/inhibitory balance, oxidative and mitochondrial stress responses, neuroinflammation/neuroimmune regulation, and aging-related neurogenesis and cognitive resilience. In chronic neurodegenerative and neurodevelopmental settings, excessive eEF2K activity is frequently associated with impaired de novo protein synthesis, synaptic dysfunction, and cognitive decline, whereas genetic or pharmacological suppression can improve selected behavioral and electrophysiological outcomes. By contrast, in acute metabolic stress or certain immune-cell contexts, eEF2K activity may serve adaptive and anti-inflammatory functions. These findings indicate that eEF2K has context-dependent, rather than uniformly pathogenic, roles. We also highlight major translational barriers, including dependence on rodent models, limited causal human data, incomplete cell-type resolution, and the off-target liabilities of commonly used inhibitors such as NH125 and A-484954. Overall, the eEF2K/eEF2 axis represents a biologically important but therapeutically complex target that will require selective, cell-aware, and stage-specific modulation. Future progress depends on better biomarkers, human models, and more selective brain-penetrant inhibitors. - Source: PubMed
Publication date: 2026/05/05
Mohammady Ruzan WSamir Rawan KSayed Rana MMalak Marina HMagdy Marian KMohamed Rawan GTawfiq Aya HKamel Rahma AKamel Nada M