NF_Y EMSA Kit
- Known as:
- NF_Y EMSA Kit
- Catalog number:
- AY1223
- Product Quantity:
- 25 rxn
- Category:
- -
- Supplier:
- Panomics
- Gene target:
- NF_Y EMSA Kit
Related genes to: NF_Y EMSA Kit
- Gene:
- NFYAP1 NIH gene
- Name:
- nuclear transcription factor Y subunit alpha pseudogene 1
- Previous symbol:
- NFYAP
- Synonyms:
- -
- Chromosome:
- 13q21.31
- Locus Type:
- pseudogene
- Date approved:
- 2008-04-18
- Date modifiied:
- 2015-11-11
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- Neuronal circuit formation is controlled by secreted guidance molecules and their receptors. However, the transcriptional mechanisms underlying neuronal circuit formation are not fully understood. In an unbiased genetic screen, we identified the Nuclear Factor-Y transcription factor (NF-Y) as a key regulator of neuron fate and axon guidance. NF-Y is a highly conserved and ubiquitous complex, composed of three subunits: NF-YA, NF-YB and NF-YC. The NF-YA subunit directly binds to regulatory regions of target genes to control their expression. Here, we show that NFYA-1 controls neurite development of multiple neuronal subtypes in Using the serotonergic neurosecretory-motor neurons (NSM) neurons as a model, we discovered that NFYA-1 acts cell autonomously to limit NSM dorsal neurite length. Further, NSM neurite overextension caused by NFYA-1 loss is suppressed by overactivating DBL-1/BMP (Bone Morphogenetic Protein) signaling. A previous study found that in , NF-YC regulates axon targeting of photoreceptor neurons. Thus, NF-Y likely plays a broad role in shaping neuronal circuitry across species, albeit through distinct mechanisms. Correct nervous system development requires precise neurite guidance and termination. Over the last four decades, the guidance molecules controlling these events have been extensively studied, whereas transcriptional regulation of these molecules remains poorly understood. We reveal an important role for the Nuclear Factor-Y transcription factor (NF-Y) in controlling distinct neurodevelopmental events. By specifically focusing on neurite extension, we show that NF-Y acts in parallel with multiple established neuronal architects to promote correct neurite development. Intriguingly, inducing the Bone Morphogenetic Protein (BMP) pathway corrects aberrant neurite extension caused by NF-Y loss. These findings provide insight into how nervous system architecture is controlled by a conserved transcriptional regulator and the crucial connection between NF-Y and the BMP pathway during neurite extension. - Source: PubMed
Publication date: 2026/05/05
Moreira PedroPapatheodorou PaulPocock Roger - The dynamics of chromatin accessibility that regulate oogenesis for the establishment of the female ovarian reserve in mammals have not been explored in reptiles with lifelong continuous oogenesis. Assays for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) were conducted to generate a chromatin accessibility landscape from gonadal samples from three critical stages of oogenesis in female Chinese alligators, which revealed a pronounced global decrease in chromatin accessibility across these developmental stages. Functional enrichment analysis demonstrated that differentially accessible regions were associated with genes involved in cell cycle regulation, meiosis, RNA binding, and cellular metabolism. Transcription factor footprinting identified krüppel-like factor (KLF) and specificity protein (SP) family members, POZ/BTB and AT-hook containing zinc finger protein 1 (PATZ1), and the nuclear transcription factor Y (NF-Y) complex (including subunits NFYA, NFYB, and NFYC) as core regulatory factors, with NF-Y binding sites exhibiting marked dynamic changes during development. Further analysis showed that NF-Y target genes are significantly enriched in critical processes such as cell cycle, mitosis, and meiosis, including key regulators Aurora Kinase A (AURKA) and AURKB. Experimental validation confirmed widespread ovarian expression of NFYA, NFYB, and NFYC, with AURKB expression strongly correlated with NF-Y protein levels. Dual-luciferase reporter assays demonstrated that NFYA significantly enhances AURKB promoter activity through two CCAAT-box elements near the transcription start site. This research depicts the chromatin accessibility dynamics during oogenesis of the Chinese alligator and pinpoints NF-Y as a crucial regulator of AURKB, thereby providing a mechanistic foundation for understanding reproduction and advancing conservation in this endangered species. - Source: PubMed
Publication date: 2026/04/29
Li PengfeiNi YutingWen YueCao XiaojingLi JinZhou YongkangYi PingsiWu XiaobingNie Haitao - Nuclear factor Y (), evolutionarily conserved heterotrimeric transcription factors (TFs), are found throughout eukaryotic organisms. Comprising the , , and subfamilies, this family is established as playing critical roles in plant growth and development. While earlier research has mainly centered on the functional and evolutionary characteristics of within individual plant species, large-scale analyses and evolutionary patterns of these genes across major plant lineages remain largely unexplored. Here, we systematically identified 15 392 nonredundant genes of family from 320 horticultural and representative plant species. Our findings showed that this gene family originated from charophytes. In bryophytes, pteridophytes, and gymnosperms, dispersed duplication served as the predominant mode of gene expansion, whereas in angiosperms, their expansion was driven by whole genome duplication/segmental, dispersed, and tandem duplication. Conserved motif analysis revealed that highly conserved motifs are present within each subfamily across eight representative plant species. However, some genes in higher plants exhibited motif loss, indicating sequence variations during their evolutionary history. Transcriptomic profiling analysis of genes in under various conditions, including hormonal treatments, abiotic/biotic stresses, as well as various developmental stages, revealed their functional versatility. Furthermore, an interaction network comprising 36 genes along with 2473 downstream and 261 upstream genes was constructed in . Enrichment analysis revealed interactions between genes and other TFs, particularly those from the _DNA-binding and APETALA2 () families, which were consistently enriched among both upstream and downstream regulatory genes. This work provides the first comprehensive and large-scale investigation into the evolutionary dynamics of genes, encompassing taxa from basal algae to advanced horticultural plants, thereby offering novel insights into their evolutionary and lineage-specific expansion. - Source: PubMed
Publication date: 2025/11/04
Luo KaiLi MingchaoLiu ManJia XitaoLi ZhouZhao XuechunChen JihuiGu XinyaoHe JinChen ChaoDong Rui - Pecan transcription factor CiNF-YC6, identified as nuclear-localized, significantly boosts fatty acid content in transgenic plants via activating lipid pathway genes upon overexpression. Pecan (Carya illinoinensis) seed oil is rich in unsaturated fatty acids beneficial for human health. Therefore, improving its quality is of considerable interest. The Nuclear Factor Y (NF-Y) transcription factor family plays essential roles in plant development and metabolism, yet its function in regulating seed lipid accumulation in woody oil-producing species remains poorly understood. Here, we identified and characterized 44 NF-Y genes in Carya illinoinensis, comprising 12 NF-YA, 20 NF-YB, and 12 NF-YC members. Phylogenetic, structural, and cis-element analyses revealed conserved features and regulatory potential across the family. Among them, CiNF-YC6 exhibited high expression in developing seeds and other tissues. Subcellular localisation and yeast assays confirmed that CiNF-YC6 is a nuclear-localized transcriptional activator with the activation domain located at the C-terminus. Functional characterization using transient expression in Nicotiana benthamiana and stable transformation in Arabidopsis thaliana showed that CiNF-YC6 overexpression significantly increased total fatty acid content and altered fatty acid composition. This enhancement was accompanied by the upregulation of key genes involved in fatty acid biosynthesis and triacylglycerol (TAG) assembly. Our findings identify CiNF-YC6 as a novel regulator of lipid accumulation and provide insights into the transcriptional control of oil biosynthesis in a woody perennial species. - Source: PubMed
Publication date: 2025/12/19
Wang LinnaZou LinaYao ZixianHe JinhuaZhang ShunranXiang Yan - Context The induction of oocytes from embryonic stem cells (ESCs) in vitro provides a promising tool for the treatment of female infertility. Various molecules are involved in this complex process, which requires further elucidation. Aims This study aims to screen for factors that induce the differentiation of ESCs into oocytes in vitro by constructing transcription factor (TF)-mediated gene regulatory networks (GRNs) during the formation of oocytes. Methods Based on publicly available multi-omics data, the weighted gene co-expression network analysis (WGCNA) method identified oocyte-specific TFs and key oocyte-specific genes. Additionally, chromatin immunoprecipitation (ChIP) sequencing data and ChIP-qPCR analysis were used to examine GRNs mediated by oocyte-specific TFs. Key results First, by analyzing assay for transposase-accessible chromatin sequencing (ATAC-seq) and DNase I hypersensitive site sequencing (DNase-seq) data from human and mouse ESCs, primordial germ cells (PGCs), and oocytes, we identified five and three oocyte-specific TFs, respectively. RNA sequencing and WGCNA further revealed 38 key oocyte-specific genes. Subsequently, when comparing cell-specific TFs in mouse and human oocytes, we identified three overlapping oocyte-specific TFs (NFYA, NFYB, and NFYC). Notably, NFYA exhibited significantly elevated expression levels in oocytes compared to ESCs and PGCs. Additionally, ChIP-qPCR results demonstrated that NFYA was relatively enriched at the promoter region of the key oocyte-specific gene, m6 A demethylase Alkbh5 . Conclusions This study provides preliminary insights into the role of cell-specific TFs and TF-mediated GRNs in oocyte formation by identifying oocyte-specific genes and key oocyte-specific TFs. Implications The findings indicate that their intricate regulatory mechanisms may significantly contribute to enhancing the efficiency of differentiating ESCs into oocytes. - Source: PubMed
Wu DiLiang ZifanLi ZiqiZhang BoyangLi QiwenShi KesongFang Shu