HOX4C EMSA Kit
- Known as:
- HOX4C EMSA Kit
- Catalog number:
- AY1193
- Product Quantity:
- 25 rxn
- Category:
- -
- Supplier:
- Panomics
- Gene target:
- HOX4C EMSA Kit
Related genes to: HOX4C EMSA Kit
- Gene:
- HOXD9 NIH gene
- Name:
- homeobox D9
- Previous symbol:
- HOX4C, HOX4
- Synonyms:
- -
- Chromosome:
- 2q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1990-06-15
- Date modifiied:
- 2015-08-25
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- To investigate the expression of homology box D9 gene (HOXD9) in gliomas and its association with clinical features and prognosis, and to study the effect of HOXD9 gene on the proliferation and migration of glioma cells. HOXD9 knockdown models were constructed in U251 and U87-MG cell lines, and the effects of HOXD9 on glioma cell proliferation and invasion were verified using CCK8, plate cloning, Transwell, and migration assays. The expression levels of HOXD9 mRNA in TCGA database were also collected, and the prognostic value of HOXD9 was evaluated by plotting the survival curves. Gene set enrichment analysis (GSEA) was used to analyze the possible mechanism of HOXD9 gene in gliomas, and to analyze the correlation between the expression of HOXD9 gene and the immune cell infiltration of gliomas. In vitro experiments revealed that knockdown of HOXD9 expression inhibited the proliferation and migration of human glioma cells U251 and U87-MG. Bioinformatics showed that HOXD9 expression was up-regulated in gliomas, and HOXD9 expression correlated with overall survival (OS). GO and KEGG enrichment analyses revealed that HOXD9 was associated with multiple biological processes and signaling pathways. And the immune infiltration analysis about HOXD9 showed that HOXD9 was associated with a variety of immune cells, suggesting that HOXD9 might alter the immune microenvironment of the tumor. HOXD9 affects glioma cell proliferation and migration, and its expression is upregulated in glioma tissues, correlating with the prognosis of glioma patients. HOXD9 may serve as a potential therapeutic target and biomarker for glioma. - Source: PubMed
Publication date: 2026/04/10
Jing ZixuanLi ChenglongLi Zefu - Recent research has underscored the crucial role of dysregulated RNA-binding proteins (RBP) in the onset and advancement of gastric cancer. Cold shock domain containing E1 (CSDE1), functioning as an RBP, has emerged as an essential contributor to this process, although its specific mechanistic roles in GC are still not fully understood. Immunohistochemistry was performed on human gastric cancer tissue microarrays to assess CSDE1 expression. Extracellular acidification rate, glucose uptake, and lactate production assays were conducted to measure glycolytic activity. RNA immunoprecipitation assays were used to examine the interaction between CSDE1 and Lactate dehydrogenase A (LDHA) mRNA. Chromatin immunoprecipitation assays were performed to detect lactylation modification at the H3K18 site within the Homeobox D9 (HOXD9) promoter region. In vitro cell assays and in vivo xenograft experiments were conducted to evaluate the biological functions of CSDE1. Our findings indicate that CSDE1 is significantly upregulated in gastric cancer tissues and correlated with poor prognosis. CSDE1 enhances LDHA mRNA stability, leading to increased glycolytic activity and lactate production. The lactate generated promotes HOXD9 transcription through H3K18 lactylation, thereby facilitating gastric cancer progression. Thus, CSDE1 drives gastric cancer progression through the LDHA-lactate-H3K18 lactylation-HOXD9 axis. CSDE1 promotes metabolic reprogramming in gastric cancer by enhancing lactate production and downstream epigenetic signaling. Its overexpression predicts poor prognosis, highlighting CSDE1 as both a potential biomarker and a therapeutic target for improving gastric cancer outcomes. - Source: PubMed
Publication date: 2026/03/20
Zhang HongyuWang XiaokunSun NanWang ZhenZhao ChunlinYin SongchengZeng LeliZhang YaweiFu Yang - Adenomyosis diagnosis and classification relies primarily on magnetic resonance imaging or pathological examination. Liquid biopsy has been widely applied in oncology but remains underexplored for adenomyosis. We aimed to identify liquid-biopsy-derived 5hmC biomarkers to aid adenomyosis diagnosis and molecular subtyping. - Source: PubMed
Publication date: 2026/03/19
Zhang LeiHan XiaotongGao XinranChen HangyuShang ChunliangChen LongLin JianGuo Hongyan - Breast cancer (BC) is the most commonly diagnosed cancer in women globally, highlighting the need for new therapeutic targets. This study aims to clarify the role of pleckstrin-2 (PLEK2) in BC progression. We particularly focus on how the transcription factor Homeobox D9 (HOXD9) regulates PLEK2 and its influence on the AKT signaling pathway. We employed quantitative PCR, Western blotting, gene knockdown and overexpression techniques, scratch assays, Transwell assays, and biochemical analyses to investigate the effects of PLEK2 on cell migration, invasion, and metabolic activity in BC cells. We found that PLEK2 was significantly overexpressed in BC tissues compared to normal tissues in the database, and this overexpression was associated with poor prognosis. Functional assays showed the modulatory effect of PLEK2 on glycolytic activity, migration, and invasion of breast cancer cells. Mechanistically, PLEK2 regulates the PI3K/AKT/mTOR pathway, HIF-1α protein levels, and the expression of its target glycolytic genes, thereby promoting glycolysis critical for cancer progression. Notably, HOXD9 was identified as a key regulator of PLEK2 expression, confirmed by luciferase assays and ChIP-qPCR. Overexpression of HOXD9 restored the migratory and invasive abilities of PLEK2-silenced cells, indicating a significant interplay between HOXD9 and PLEK2 in breast cancer. In conclusion, our study highlights the pivotal role of PLEK2 in BC cell dynamics and introduces HOXD9 as a novel regulator of PLEK2. Targeting the HOXD9/PLEK2/AKT signaling axis may offer a promising therapeutic strategy for treating breast cancer. - Source: PubMed
Publication date: 2025/10/24
Xiao MengYin ZhijieWu HuaWang LijingShi Yuanyuan - Kazakh horses, a distinguished breed in China known for their dual-purpose use in milk and meat production, exhibit early maturation, tolerance to coarse feeding, and strong resistance to environmental stress. However, the gene expression differences across various muscle tissues of Kazakh horses have yet to be elucidated. In this study, transcriptomic sequencing was performed on muscle tissues from three anatomical regions of Kazakh horses, including the longissimus dorsi (Gb), external oblique (Gf), and diaphragm (Gg) muscles. In the Gb and Gf groups, 426 differentially expressed genes (DEGs) were identified, including , and , of which 147 were upregulated and 279 downregulated. In the Gf and Gg groups, 1,762 DEGs were detected, including , and , with 1,391 upregulated and 371 downregulated. Additionally, 644 DEGs were identified between the Gg and Gb groups, including , and , with 172 upregulated and 472 downregulated. GO annotation and KEGG enrichment analysis revealed that the DEGs, such as , and , were primarily involved in System Development, Extracellular Space, and Protein-Arginine Deiminase Activity. Furthermore, pathways related to skeletal muscle growth, including Cytoskeleton in Muscle Cells, Cytokine-Cytokine Receptor Interaction, and Motor Proteins, were significantly enriched. RT-qPCR analysis validated the accuracy of the transcriptomic sequencing data. This study provides valuable insights into the differential expression of genes and related signaling pathways in various muscle tissues of Kazakh horses, rendering a theoretical foundation and data references for understanding skeletal muscle growth and improving meat production in equines. - Source: PubMed
Publication date: 2025/07/24
Wubulikasimu MierkadinaLiu JiahaoYao XinkuiMeng JunWang JianwenZeng YaqiLi LinlingRen Wanlu