GATA4 EMSA Kit
- Known as:
- GATA4 EMSA Kit
- Catalog number:
- AY1097
- Product Quantity:
- 25 rxn
- Category:
- -
- Supplier:
- Panomics
- Gene target:
- GATA4 EMSA Kit
Related genes to: GATA4 EMSA Kit
- Gene:
- GATA4 NIH gene
- Name:
- GATA binding protein 4
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 8p23.1
- Locus Type:
- gene with protein product
- Date approved:
- 1994-11-30
- Date modifiied:
- 2016-10-05
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- Fatty liver hemorrhagic syndrome (FLHS) is a metabolic disease of laying hens that reduces egg production and is accompanied by reproductive impairment, but the ovarian regulatory mechanisms that connect nutritional stress to follicular dysfunction remain unclear. This study examined whether active chromatin remodeling in the ovary is associated with FLHS induced by a high-energy, low-protein (HELP) diet. Hy-Line Brown hens were assigned to a basal diet or HELP diet, and ovarian tissue was collected for histone H3 lysine 27 acetylation (H3K27ac) chromatin immunoprecipitation sequencing and RNA sequencing. The HELP diet reduced laying performance and the numbers of small yellow and hierarchical follicles, indicating compromised follicular development. Genome-wide H3K27ac profiling identified 2,111 regions with lower acetylation and 1,707 regions with higher acetylation in FLHS ovaries. Genes linked to differential H3K27ac regions were enriched in pathways related to oocyte meiosis, cell cycle control, FoxO signaling, gonadotropin-releasing hormone signaling, and steroid hormone biosynthesis. RNA sequencing identified 341 differentially expressed genes, with a predominance of downregulated genes. Integration of chromatin and transcriptome data highlighted folliculogenesis-related genes, including FGF1, FGF9, and MMP10, that showed reduced H3K27ac enrichment together with decreased expression. Super-enhancer analysis further identified 131 regions with reduced H3K27ac signal in FLHS ovaries, including regions located near PCNA and RAP1A, two genes involved in cellular proliferation and survival signaling. Motif enrichment of differential H3K27ac regions implicated Fos, SF-1/NR5A1, and GATA-4 as candidate transcriptional regulators. These findings indicate that HELP diet-induced FLHS is associated with broad attenuation of active ovarian regulatory elements and reduced expression of genes required for follicle growth, tissue remodeling, and steroidogenic function. The study provides an ovarian epigenomic framework for understanding reproductive decline in FLHS-affected laying hens. - Source: PubMed
Publication date: 2026/07/05
Cui YongZeng WenhuiLiang HaipingWang YujieWeng LinjianQu MingWei QingXie XianhuaHuang Jianzhen - Epigenetic modulators such as histone deacetylases (HDACs) and histone acetyltransferases (HATs) are known master regulators of gene expression that substantially impact cardiac electrophysiology. Novel pharmacological agents, HDAC inhibitors, are rapidly emerging as treatments for cancer and immune diseases, and their effects on cardiac ion channels (ICs) are of great interest. We used small interfering RNAs to individually suppress each of the known HDACs, including sirtuins (SIRTs), in human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs), iCell2. Follow-up deep-sequencing allowed comparison to identically processed and normalized RNA sequencing data from adult human left ventricle (LV) from the GTEx database. The transcriptomics analysis revealed high similarity of gene expression patterns for cardiac ICs (with some differences in calcium influx and calcium buffering related genes), as well as strong co-regulation by cardiac transcription factors (TFs) and in both hiPSC-CMs and the adult LV. Partial least square regression models helped visualize links between HDACs/HATs, TFs, and cardiac ICs and helped identify potential key regulators of cardiac IC transcription. Powerful TFs, including exerted a positive effect on IC genes while and were distinct negative regulators in both sample types; was found to serve opposite roles regulating ICs in the hiPSC-CM compared to the adult LV. In functional measurements, suppression primarily increased excitability, while suppression decreased excitability, in line with transcriptomic links and in qualitative agreement with predictions by a computational ionic model of hiPSC-CMs. Our analysis offers insights about the role of epigenetic modifiers in regulating cardiac electrophysiology and informs the utility of hiPSC-CM as a scalable experimental model for cardiotoxicity testing of HDAC inhibitors. - Source: PubMed
Publication date: 2026/03/18
Pozo Maria RPressler Michael PHorvath AneliaEntcheva Emilia - Testicular tumors are rarely reported in passerine birds and are typically described as isolated cases. This study characterizes the clinical, pathological, immunohistochemical, and epidemiological features of 13 testicular neoplasms diagnosed in ultramarine grosbeaks ( = 12) and a green-winged saltator ( = 1), 2 South American passerine species commonly used in singing competitions. Clinical signs included coelomic distension, respiratory distress, pododermatitis, feather loss, cloacal prolapse, and decreased vocalization. Radiography demonstrated displacement of coelomic organs and, in some cases, markedly reduced gastrointestinal transit associated with tumor compression. Grossly, tumors were large, pale tan to yellow, frequently multilobulated, and caused compression of adjacent viscera. Histologically, all neoplasms were classified as sustentacular cell tumors (SCTs) based on established histomorphological criteria adapted for avian testes. Most tumors exhibited a tubular growth pattern (77%), whereas diffuse (15%) and mixed-type (7%) patterns were less common. Rare Call-Exner-like bodies and extensive contralateral testicular atrophy were observed. Immunohistochemically, KIT (CD117) and neuron-specific enolase were the most consistently expressed markers, whereas melan-A, calretinin, and GATA-4 showed sporadic labeling and inhibin-α, vimentin, and pancytokeratin were consistently negative. Liver metastases occurred in 2 ultramarine grosbeaks, demonstrating the malignant potential of SCTs in passerines. This study represents the largest series of testicular tumors reported in passerine birds and provides the first evidence that participation in singing competitions is significantly associated with the development of SCTs in ultramarine grosbeaks. Our results underscore the importance of including testicular neoplasia as a differential diagnosis in cases of coelomic distension in passerines. - Source: PubMed
Publication date: 2026/08/25
Oliveira Filho Hodias S deDuarte José L CostaParanhos Gabriel FSilva João Paulo G daNeto Paulo M da NóbregaLins Bianca BM Dos Santos WellingtonL de Oliveira RafaelSantos Renato LPavarini Saulo PetinattiTsoi Mayra FAgnew DalenLeal de Araújo Jeann - Osimertinib, a third-generation EGFR-TKI, is now the standard first-line treatment for EGFR-mutant non-small cell lung cancer and is increasingly given in the adjuvant setting for up to 3 years and in combination with chemotherapy. As exposure has lengthened and shifted toward curative-intent settings, its cardiac effects have acquired greater clinical weight, because patients may remain on therapy for years and survive long enough for cardiovascular outcomes to matter. This review argues that the dominant cardiac phenotype, a decline in left ventricular ejection fraction, with or without clinical heart failure, is best understood as an often reversible functional cardiomyopathy rather than structural myocardial injury. The neuregulin-1/ErbB signaling axis, which maintains adult cardiomyocyte function and whose disruption by trastuzumab causes a comparable reversible dysfunction, provides the lens for this interpretation, and off-target inhibition of ErbB2/ErbB4 has been proposed as a mechanism for osimertinib cardiotoxicity. That account is incomplete: the EGFR-TKIs that inhibit HER2 most potently do not carry the largest clinical signal, and a recently described GATA4-MYLK3-MYL2 contractile pathway offers an alternative, not clearly ErbB-dependent, mechanism. The proximal cause, therefore, remains unresolved, but across the available evidence, the dominant phenotype appears functional, contractile, and often reversible. - Source: PubMed
Publication date: 2026/08/24
Gopu Sooraj SrirangadhamuKhader Abul Hasan Shadali AbdulAgrawal Siddharth PravinJajja Salman AyubPatel Anjali PareshbhaiFrishman William HAronow Wilbert S - Genetic hypertrophic and dilated cardiomyopathies (HCM and DCM, respectively) are characterised by structural and functional abnormalities that can lead to heart failure. However, current therapies mainly reduce symptoms. Patient-derived human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes provide a valuable platform to study genotype-specific pathophysiology and pharmacology. We subjected hiPSC-cardiomyocytes from healthy individuals and from patients carrying pathogenic MYBPC3 (HCM) or LMNA (DCM) mutations to cyclic mechanical stretch, with or without the GATA4-targeted anti-hypertrophic compound 3i-1262, and assessed hypertrophy-associated, mechanosensitive and metabolism-related genes by qPCR, and hypertrophy-related proteins by Western blotting. Compared to control, HCM cardiomyocytes displayed higher basal expression of NPPB and MYH7, whereas DCM cardiomyocytes exhibited lower basal expression of NPPB and NPPA. Mechanical stretching induced NPPB and MYH7 upregulation in control cardiomyocytes, delayed MYH7 upregulation in HCM cardiomyocytes and NPPA downregulation in DCM cardiomyocytes. Other mechanosensitive genes, such as GAL, CSRP3 and SLC16A9, also exhibited genotype- and time-dependent regulation. In control cardiomyocytes, 3i-1262 produced limited modulation of stretch-induced gene expression but showed little or no effect in patient-derived cardiomyocytes. These findings demonstrate that cardiomyopathy mutations influence gene and protein expression, responses to mechanical stretch and 3i-1262, underscoring the value of patient-derived hiPSC-cardiomyocytes in disease modelling and drug discovery. - Source: PubMed
Pohjavaara SaanaKinnunen Sini MRuskoaho HeikkiAalto-Setälä KatriinaVälimäki Mika JTalman Virpi