Anti-Mouse CD8b PE 200 ug
- Known as:
- Antibody toMouse CD8b PE 200 ug
- Catalog number:
- 12-0083-83
- Category:
- -
- Supplier:
- eBioscience
- Gene target:
- Anti-Mouse CD8b 200
Related genes to: Anti-Mouse CD8b PE 200 ug
- Gene:
- CD8B NIH gene
- Name:
- CD8b molecule
- Previous symbol:
- CD8B1
- Synonyms:
- -
- Chromosome:
- 2p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 1989-04-13
- Date modifiied:
- 2016-10-05
Related products to: Anti-Mouse CD8b PE 200 ug
Related articles to: Anti-Mouse CD8b PE 200 ug
- Chronic hepatitis B virus (HBV) infection is a major etiological driver of hepatocellular carcinoma (HCC) and is accompanied by profound immune dysregulation that shapes disease progression and therapeutic responsiveness. However, the extent to which systemic immunity reflects the tumor immune microenvironment (TIME) in HBV-related HCC (HBV-HCC) remains incompletely defined. Here, we used mass cytometry (CyTOF) to resolve immune heterogeneity across peripheral blood mononuclear cells (PBMCs), tumor tissues, and matched adjacent non-tumor (paracancer) tissues in treatment-naïve HBV-HCC. PBMCs, tumor tissues, and paracancer tissues were collected from 12 treatment-naïve HBV-HCC patients and profiled by CyTOF. Nine major immune lineages/clusters were quantified and compared across compartments. Immune-cell distributions were correlated with virological and clinicopathological features (HBV DNA status, serum alpha-fetoprotein [AFP], tumor differentiation) and 5-year clinical outcomes. Public transcriptomic datasets were further leveraged for external validation of CD8A/CD8B-associated prognostic signals. HBV-HCC exhibited marked systemic-local immune compartmentalization. PBMCs were enriched for naïve CD8⁺ T cells and natural killer (NK) cells, whereas paracancerous tissues showed higher abundance of myeloid-derived suppressor cells (MDSCs) and memory CD8⁺ T cells. In contrast, HCC tissues were characterized by increased neutrophils and regulatory T (Treg) cells, together with a more activated and immunosuppressive marker profile in HCC-associated MDSCs. HBV DNA-positive patients showed higher intratumoral expression of naïve CD8⁺ T cells and Tregs than HBV DNA-negative patients, with the increase being most evident in HCC tissues relative to matched paracancerous tissues. Clinically aggressive phenotypes, including high AFP, poor differentiation, and postoperative recurrence, were characterized by neutrophil expansion accompanied by reduced PD-1⁺ dendritic cells (PD-1⁺ DCs) and decreased naïve/memory CD8⁺ T-cell subsets. Although higher CD8A/CD8B expression in public datasets predicted improved survival, CyTOF indicated that abundant intratumoral CD8⁺ infiltration could coexist with systemic and intratumoral immunosuppression and functional exhaustion, consistent with the recognized challenge of reinvigorating exhausted intrahepatic immunity. HBV-HCC is defined by profound immune heterogeneity across blood, tumor, and adjacent non-tumor compartments. Distinct immune signatures associate with virological activity, tumor aggressiveness, and long-term outcomes, providing a rationale for immune-based patient stratification and for combinatorial immunotherapy strategies targeting both myeloid-driven suppression and dysfunctional T-cell immunity. - Source: PubMed
Publication date: 2026/07/02
Li YadiWang WenjingZeng AjuanZheng XiaoqinLyu LingnaDing HuiguoWang Shanshan - BH3 mimetics are apoptogenic but rarely cause immunogenic cell death (ICD), limiting durable antitumor immunity. We hypothesized that an ICD-inducing immunoadjuvant could convert BH3-mimetic-triggered tolerogenic apoptosis into immunogenic priming, enhancing checkpoint immunotherapy and overcoming immune evasion in AML. We in vivo evaluated the hydroxycoumarin OT-55, combined with the Bcl-xL inhibitor A-1331852, to enhance PD-1/Tim-3 blockade in Bcl-xL-dependent murine prophylactic and bilateral AML vaccination models. To define the clinical and immunological context, we derived a nine-gene AML ICD score (ATG5, CALR, CD8A, CD8B, IFNGR1, IL1B, PDIA3, PIK3CA, TLR4) by screening 34 ICD-associated genes in transcriptomes of three AML patient cohorts (TARGET-AML, BEAT-AML, GSE37642), retaining genes consistently associated with favorable prognosis (HR < 1, Cox regression). These cohorts were dichotomized by median ICD score to infer immune composition (CIBERSORTx) and profile driver mutations, and to assess blast maturation. High ICD scores were associated with an immune-activated state, increased CD8⁺ T cells, activated dendritic cells, and higher HAVCR2 (Tim-3) expression, consistent with a survival advantage. Bone marrow scRNA-seq from AML and healthy donors revealed ICD-related and progenitor-to-intermediate exhausted T cells, alongside T cell depletion in myelomonocytic AML. Experimentally, we used murine C1498 myelomonocytic AML cells to evaluate OT-55 combined with A-1331852 by prophylactic whole-cell vaccination for DAMP release, dependency testing (CRT neutralization and apyrase), antigen-specific CD8⁺ responses, and synergy with anti-PD-1/anti-Tim-3 therapy in a bilateral tumor model, while monitoring hematologic and serum parameters. OT-55 reduced C1498 viability, induced CRT exposure and ATP release, and conferred CRT/ATP-dependent, but HMGB1-independent, vaccine protection. While A-1331852 was cytotoxic yet weakly immunogenic, its combination with OT-55 enhanced DAMP release, increased CD8⁺ effector functions, and, with PD-1/Tim-3 blockade, achieved local and distant tumor control with low toxicity. These findings identify OT-55 as an immunogenic adjuvant converting tolerogenic BH3 mimetic-driven apoptosis into ICD, providing a proof-of-concept immunogenic treatment for myelomonocytic AML. - Source: PubMed
Publication date: 2026/07/01
Lee YejinKwon Eun-JiGajulapalli Sruthi ReddyPaik Ji YeonOrlikova-Boyer BarboraSilva Artur M SCha Hyuk-JinCerella ClaudiaDiederich Marc - The role of heritable genetic variation in hematologic traits in adults is well established, yet the genetic architecture of neonatal blood cell traits is unknown. Leveraging flow cytometry profiling in cord blood samples from 382 Hispanic newborns, we conducted genome-wide association studies (GWASs) of 24 blood cell phenotypes to assess the impact of genetic and birth-related characteristics. We identified six genome-wide significant loci, including a signal at chromosome 2p11.2 spanning CD8A and CD8B that was associated with CD4CD8 double-positive T cells (rs35505884-C: p = 6.83 × 10). The lead variant showed strongly differentiated allele frequencies between European (22.1%) and admixed American (11.8%) populations and was associated with global (p = 2.51 × 10) and local (p = 2.01 × 10) Indigenous American ancestry. Colocalization with single-cell expression quantitative trait loci (eQTLs) revealed convergence of GWAS effects with regulation of CD8A expression in natural killer cells and CD4 effector memory T cells with posterior probability (PP) >0.85. A locus at 6p21.33 was associated with CD4CD8 cytotoxic T cells (rs2853973-C, p = 3.90 × 10) and colocalized with MICA-AS1 expression across multiple immune cell types (PP > 0.85). Additional nominal genome-wide significant loci were detected for total CD45 lymphocytes, CD19CD56CD16 natural killer cells, and CD4CD8 helper T cells. C-section delivery and biological sex significantly altered blood cell profiles, with the largest magnitudes of association observed between C-section and total CD3 T cells (β = 0.39) and CD4 helper T cells (β = 0.39), and between female sex and CD4CD8 (β = 0.39) and CD4CD8 T cells (β = -0.36). Our study demonstrates that blood cell composition at birth is under strong genetic regulation in Hispanic newborns, highlighting the value of studying complex traits in early life. - Source: PubMed
Publication date: 2026/07/01
Li YunqiAlonzo BenjaminZhang Zixuan ELangie JalenMyint Swe SweInce MichaelaLu RongChiang Charleston W KGazal StevenMancuso NicholasKachuri Lindade Smith Adam J - In the last decade, methods to investigate the immune status of the tumor microenvironment (TME) and predict immune checkpoint blockade efficacy have been intensively developed as immunoscores or immune signatures. However, since the immunological gene signature is a complex and time-consuming approach, a simpler scoring system using numerical data is needed to evaluate a large number of tumors. We previously established a TME immune-type classification system based on PD-L1 and CD8B gene expression, and 5032 cancer patients were classified into 4 types. Based on the expression levels of immune response-associated genes in each type, these genes were assigned scores ranging 1 to 4, representing a spectrum from immunosuppressive to immunostimulating genes. We herein calculated tumor immune status scoring algorithm (TIMMUSCORA) scores based on the expression data of 300 immune response-associated genes in 5,013 pancancer patients, and investigated the relationship of these scores with the prognosis and other clinicopathological features of cancer patients. Rectal cancers with higher scores than the cut-off value of 0 showed a good prognosis, which was closely associated with the immune response-associated genes PDCD1, GZMB, TNFRSF10C, EBI3, and IRF1. A correlation analysis of the rectal cancer cohort suggested that high TIMMUSCORA scores correlated with high TMB value and the consensus molecular subtyping 1 status, while low scores correlated with WNT gene expression. Therefore, the TIMMUSCORA system has potential in evaluations of the immune status of the TME and the prognosis of solid cancers. - Source: PubMed
Publication date: 2026/04/25
Ikeya TomoatsuKikuchi YasufumiAshizawa TadashiKanematsu AkariMaeda ChieIizuka AkiraYamashita KazueMiyata HaruoNagashima TakeshiUrakami KenichiShimoda YujiOhshima KeiichiMuramatsu KojiSugino TakashiShiomi AkioOhde YasuhisaBando EtsuroSugiura TeiichiMukaigawa TakashiNishimura SeiichiroKenmotsu HirotsuguYamaguchi KenAkiyama Yasuto - Compared with transplanted tumors, autochthonous tumors are difficult to cure using experimental radiation therapy in mice. Here we analyzed differences in immune-related gene expression profiles between mouse fibrosarcomas subcutaneously induced by 3-methylcholanthrene (3MC) and their corresponding transplanted tumors. The immune genes examined were Pd1, Pdl1, Pdl2, Cd3d, Cd8a, Cd8b, Ifnγ, Itga2, Gzmb, and Foxp3. Among 12 tumors, one was non-transplantable and showed a benign phenotype with an abundance of DX5+ natural killer cells and CD8+ T cells together with increased IFNγ expression and mRNA levels of all immune genes except for Itga2. The other 11 transplantable tumors showed increased expression of Pd1, Pdl1, Pdl2, Cd3d, Cd8b, and Ifnγ following transplantation into syngeneic mice. These effects of transplantation highlight the relevance of immune gene expression status to the curability of tumors. - Source: PubMed
Publication date: 2026/04/24
Tanooka HiroshiKudo-Saito ChieChiwaki FumikoIshiai MasamichiTatsumi KouichiSasaki HirokiOchiya Takahiro