Anti-Mouse CD1d PE 100 ug
- Known as:
- Antibody toMouse CD1d PE 100 ug
- Catalog number:
- 12-0011-82
- Category:
- -
- Supplier:
- eBioscience
- Gene target:
- Anti-Mouse CD1d 100
Related genes to: Anti-Mouse CD1d PE 100 ug
- Gene:
- CD1D NIH gene
- Name:
- CD1d molecule
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q23.1
- Locus Type:
- gene with protein product
- Date approved:
- 1990-06-11
- Date modifiied:
- 2015-08-27
Related products to: Anti-Mouse CD1d PE 100 ug
Related articles to: Anti-Mouse CD1d PE 100 ug
- Non-Small-Cell Lung Cancer (NSCLC) often responds poorly to immune checkpoint blockade due to its immunosuppressive, "cold" tumor microenvironment. Activating alternative immune effectors may overcome this limitation. Here we identified lung-enriched γδ T cells as a key compartment in NSCLC and developed a lung-targeted lipid nanovaccine to activate them in situ. Analysis of patient transcriptomic data sets reveals that γδ T cell and CD1d signatures are associated with improved patient survival in NSCLC. Using this insight, we engineered α-galactosylceramide (α-GalCer) and poly(I:C)-loaded lipid nanoparticles that preferentially accumulated in the lung after intravenous administration. In orthotopic NSCLC models, the nanovaccine activated γδ T cells, enhanced functional CD8 T cell infiltration, remodeled the immunosuppressive tumor microenvironment, and significantly prolonged survival. Depletion of γδ T cells abolished therapeutic benefit, demonstrating that γδ T cells represented the important effector population for this strategy. Furthermore, splenectomy attenuated vaccine efficacy, suggesting a contribution of systemic immune crosstalk to vaccine efficacy. Together, these findings establish a γδ T cell-centered lung-targeted immunotherapy strategy for treating immune-resistant NSCLC. - Source: PubMed
Publication date: 2026/07/07
Yang ZeyuLi LiyanHe ZepengChen HaolinWen ZhenfuZhang ZhihuiLiu HongLiu LixinChen Yongming - Unconventional T cells, including gamma delta (γδ) T cells, mucosal-associated invariant T (MAIT) cells, and CD1d-restricted natural killer T (NKT) cells, comprise a unique component of the immune system. These cells play critical roles in host defense, immune regulation, and disease pathogenesis. In particular, invariant NKT (iNKT) cells have emerged as key immunological orchestrators that bridge innate and adaptive immunity by rapidly recognizing lipid antigens presented by CD1d molecules. This chapter provides a comprehensive overview of iNKT cell biology, including their development, phenotypic and functional heterogeneity, and activation mechanisms. It discusses the role of iNKT cells in pathological conditions in detail. Their ability to directly kill tumor cells, as well as their potential to orchestrate the immune response, opens up new possibilities for anti-cancer therapy. Their contribution to immune response regulation and tolerance highlights their critical role in infectious diseases and transplantation. The chapter summarizes current clinical approaches aimed at harnessing iNKT cells, including in vivo activation strategies, adoptive transfer of ex vivo-expanded cells, and developing chimeric antigen receptor (CAR)-engineered iNKT cells. These emerging therapies have advantages over conventional CAR-T approaches, such as reduced toxicity and the potential for allogeneic use. Finally, the chapter discusses the role of type II NKT cells and their cross-regulatory interactions with iNKT cells. Overall, CD1d-restricted NKT cells are a promising target for next-generation immunotherapies. However, further mechanistic and clinical studies are needed to realize their full therapeutic potential. - Source: PubMed
Publication date: 2026/05/21
Holubová MonikaCaputo Galarce Valentina SofiaČedíková Miroslava - This study aims to explore the pathogenesis of the comorbidity of atherosclerosis(AS) accompanied by seborrhea and the intervention effect of Zhuyu Pills. A mouse model of AS with seborrhea was constructed by feeding ApoE~(-/-)mice with a high-fat diet for 12 weeks. The 50 successfully modeled ApoE~(-/-)mice were randomly assigned into 5 groups: model, low-dose, medium-dose, and high-dose(130. 54, 261. 08, and 522. 16 mg·kg~(-1), respectively) Zhuyu Pills, and atorvastatin calcium(10. 40 mg·kg~(-1)), with 10 mice in each group. Ten normal C57BL/6J mice were used as the blank control group. The blank control group and the model group were administrated with an equal volume of normal saline by gavage, while the other groups were administrated with corresponding drugs by gavage once a day for 12 weeks. After the administration was completed, hematoxylin-eosin(HE) staining was performed to observe the pathological changes of the aorta and auricle, and the percentage of aortic plaque area and the inflammation score of the auricle were calculated. The levels of total cholesterol(TC), triacylglycerol(TG), low-density lipoprotein cholesterol(LDL-C), and fasting blood glucose( FBG) in the serum were quantified by biochemical methods. The levels of free non-esterified fatty acids(NEFA) and TG in the auricular sebaceous glands were measured by colorimetric methods. The levels of helper T cell 1(Th1)-type pro-inflammatory cytokines [tumor necrosis factor-α(TNF-α) and interleukin(IL)-6], Th2-type anti-inflammatory cytokines(IL-4 and IL-10), and fasting insulin( FINS) in the serum were assessed by enzyme-linked immunosorbent assay( ELISA). The homeostatic model assessment of insulin resistance(HOMA-IR) was calculated. The changes of invariant natural killer T(iNKT) cells in the thymus, spleen, and liver were detected by flow cytometry. The expression of differentiation cluster 1d(CD1d) in the thymus, spleen, and liver was detected by immunohistochemistry. The protein levels of TNF-α, IL-6, IL-4 and IL-10 in the liver were quantified by Western blot. The results showed that compared with the blank control group, the model group exhibited extensive thickening of the aortic intima, an increase in the percentage of aortic plaque area(P<0. 01), extensive infiltration of inflammatory cells in the auricle, an increase in the inflammation score(P<0. 01), elevated levels of TC, TG, LDL-C, and FBG in the serum(P<0. 01) and NEFA, TG in the auricle(P< 0. 01), raised serum levels of TNF-α, IL-6 and an increase in HOMA-IR(P< 0. 01), lowered serum levels of IL-4, IL-10, and FINS(P<0. 01), an increase in CD1d expression in the thymus, spleen, and liver(P<0. 01), an increase in the proportion of iNKT cells(P<0. 01), up-regulation in the protein levels of TNF-α and IL-6(P<0. 01), and down-regulation in the protein levels of IL-4 and IL-10 in the liver(P<0. 01). Compared with the model group, low, medium, and high doses of Zhuyu Pills as well as atorvastatin calcium decreased the enriched area of aortic plaques and the percentage of plaque area(P<0. 01), attenuated the infiltration of inflammatory cells in the auricle, and reduced the inflammation score(P<0. 01), lowered the levels of TC, TG, LDL-C, and FBG in the serum(P<0. 05, P<0. 01) and NEFA, TG in auricular sebaceous glands(P<0. 05, P<0. 01), lowered the serum levels of TNF-α, IL-6 and decreased the HOMA-IR(P<0. 05, P<0. 01), raised the serum levels of IL-4, IL-10, and FINS(P<0. 05, P<0. 01), inhibited the expression of CD1d in the thymus, spleen, and liver(P<0. 01), decreased the proportion of iNKT cells(P<0. 01), down-regulated the protein levels of TNF-α and IL-6(P<0. 01), and up-regulated the protein levels of IL-4 and IL-10 in the liver(P<0. 01). In conclusion, Zhuyu Pills demonstrate definite therapeutic effects on aortic plaques and seborrhea in mice and can alleviate inflammatory responses by regulating the CD1d/iNKT cell axis-mediated Th1/Th2 balance. - Source: PubMed
Song WeiTeng Zheng-WenZhang Zhong-YiDang Si-JieShen TaoXiao MinZhang Yong - Vγ9Vδ2-T cells form a conserved T-cell subset known for its potent intrinsic antitumor activity and versatility in recognizing diverse cancer types independently of the major histocompatibility complex. Previously, we reported the preclinical activity of a bispecific T-cell engager (bsTCE) specific for both CD1d and the Vδ2-TCR that engaged both Vγ9Vδ2-T and type 1 natural killer T cells to CD1d-expressing hematological malignancies, including multiple myeloma (MM) and acute myeloid leukemia (AML). Here, we evaluated whether various standard-of-care drugs for patients with MM and AML/myelodysplastic syndromes (MDS) affected the in vitro antitumor activity of CD1d-Vδ2 bsTCE-activated Vγ9Vδ2-T cells. - Source: PubMed
Publication date: 2026/06/28
de Jong MilonVeth MyrtheBrachtlová TerezaKing Lisa ASaura-Esteller JoséBechler Tamara Mvan Helden Pauline MAlhan CananLameris Roelandde Gruijl Tanja Dvan der Vliet Hans J - CD1d-restricted invariant natural killer (iNK) T cells are innate T cells known for their ability to shape adaptive immunity toward inflammation or immune-suppression via the rapid production of Th1-, Th2-, and Th17-type cytokines from corresponding iNKT subsets such as NKT1, NKT2, and NKT17. IL-10-producing invariant NKT cells, termed NKT10 cells, are thought to play an immunoregulatory role, but their potential clinical use remains underexplored. We characterized human NKT10 cells from cord-derived iNKT cells and investigated their therapeutic utility in allogeneic stem cell transplantation. Cord and cord-derived iNKT cells contained a high frequency of CD4CD25CD161FoxP3 iNKT cells and showed Th2/Th10-biased cytokine production upon antigenic stimulation. Accordingly, cord-derived iNKT cells displayed a distinct gene expression profile with upregulated genes related to NKT2, NKT10, and regulatory T cells compared with adult donor-derived iNKT cells. Furthermore, single-cell RNA sequencing analysis of cord-derived iNKT cells confirmed the presence of NKT10-like subset that was enriched with multiple immunoregulatory pathways and genes related to immune-checkpoints (, , , and ) and NKT10 (, , and ), whereas the NKT1/17-like subset present in adult donor-derived iNKT cells showed upregulation of genes related to cytotoxicity (, , and ), NKR (, , , and ), NKT1 ( and ), and NKT17 (). Lastly, cord-derived iNKT cells suppressed alloreactive T cell proliferation and ameliorated xenogeneic graft-versus-host disease where the immunodeficient NSG mice received human peripheral blood mononuclear cells supplemented with cord-derived iNKT cells. Thus, NKT10-enriched, cord-derived iNKT cells are candidate cell therapeutics for immune-modulation in allogeneic stem cell transplantation and other autoimmune diseases. - Source: PubMed
Publication date: 2026/06/10
Trujillo-Ocampo AbelBorges PamellaGrefe MaisonVaz de Freitas MartielaLee Sung-EunQi YuanClinton JelitaLi DanHe HongYu LingPeris-Cuesta ArnauEhli Erik AMa QingSu XiaopingAmaral Antunes DinlerAl-Atrash GheathMolldrem Jeffrey JShpall Elizabeth JIm Jin S