Anti-Mouse CD1d PE 50 ug
- Known as:
- Antibody toMouse CD1d PE 50 ug
- Catalog number:
- 12-0011-81
- Category:
- -
- Supplier:
- eBioscience
- Gene target:
- Anti-Mouse CD1d 50
Related genes to: Anti-Mouse CD1d PE 50 ug
- Gene:
- CD1D NIH gene
- Name:
- CD1d molecule
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q23.1
- Locus Type:
- gene with protein product
- Date approved:
- 1990-06-11
- Date modifiied:
- 2015-08-27
Related products to: Anti-Mouse CD1d PE 50 ug
Related articles to: Anti-Mouse CD1d PE 50 ug
- This study aims to explore the pathogenesis of the comorbidity of atherosclerosis(AS) accompanied by seborrhea and the intervention effect of Zhuyu Pills. A mouse model of AS with seborrhea was constructed by feeding ApoE~(-/-)mice with a high-fat diet for 12 weeks. The 50 successfully modeled ApoE~(-/-)mice were randomly assigned into 5 groups: model, low-dose, medium-dose, and high-dose(130. 54, 261. 08, and 522. 16 mg·kg~(-1), respectively) Zhuyu Pills, and atorvastatin calcium(10. 40 mg·kg~(-1)), with 10 mice in each group. Ten normal C57BL/6J mice were used as the blank control group. The blank control group and the model group were administrated with an equal volume of normal saline by gavage, while the other groups were administrated with corresponding drugs by gavage once a day for 12 weeks. After the administration was completed, hematoxylin-eosin(HE) staining was performed to observe the pathological changes of the aorta and auricle, and the percentage of aortic plaque area and the inflammation score of the auricle were calculated. The levels of total cholesterol(TC), triacylglycerol(TG), low-density lipoprotein cholesterol(LDL-C), and fasting blood glucose( FBG) in the serum were quantified by biochemical methods. The levels of free non-esterified fatty acids(NEFA) and TG in the auricular sebaceous glands were measured by colorimetric methods. The levels of helper T cell 1(Th1)-type pro-inflammatory cytokines [tumor necrosis factor-α(TNF-α) and interleukin(IL)-6], Th2-type anti-inflammatory cytokines(IL-4 and IL-10), and fasting insulin( FINS) in the serum were assessed by enzyme-linked immunosorbent assay( ELISA). The homeostatic model assessment of insulin resistance(HOMA-IR) was calculated. The changes of invariant natural killer T(iNKT) cells in the thymus, spleen, and liver were detected by flow cytometry. The expression of differentiation cluster 1d(CD1d) in the thymus, spleen, and liver was detected by immunohistochemistry. The protein levels of TNF-α, IL-6, IL-4 and IL-10 in the liver were quantified by Western blot. The results showed that compared with the blank control group, the model group exhibited extensive thickening of the aortic intima, an increase in the percentage of aortic plaque area(P<0. 01), extensive infiltration of inflammatory cells in the auricle, an increase in the inflammation score(P<0. 01), elevated levels of TC, TG, LDL-C, and FBG in the serum(P<0. 01) and NEFA, TG in the auricle(P< 0. 01), raised serum levels of TNF-α, IL-6 and an increase in HOMA-IR(P< 0. 01), lowered serum levels of IL-4, IL-10, and FINS(P<0. 01), an increase in CD1d expression in the thymus, spleen, and liver(P<0. 01), an increase in the proportion of iNKT cells(P<0. 01), up-regulation in the protein levels of TNF-α and IL-6(P<0. 01), and down-regulation in the protein levels of IL-4 and IL-10 in the liver(P<0. 01). Compared with the model group, low, medium, and high doses of Zhuyu Pills as well as atorvastatin calcium decreased the enriched area of aortic plaques and the percentage of plaque area(P<0. 01), attenuated the infiltration of inflammatory cells in the auricle, and reduced the inflammation score(P<0. 01), lowered the levels of TC, TG, LDL-C, and FBG in the serum(P<0. 05, P<0. 01) and NEFA, TG in auricular sebaceous glands(P<0. 05, P<0. 01), lowered the serum levels of TNF-α, IL-6 and decreased the HOMA-IR(P<0. 05, P<0. 01), raised the serum levels of IL-4, IL-10, and FINS(P<0. 05, P<0. 01), inhibited the expression of CD1d in the thymus, spleen, and liver(P<0. 01), decreased the proportion of iNKT cells(P<0. 01), down-regulated the protein levels of TNF-α and IL-6(P<0. 01), and up-regulated the protein levels of IL-4 and IL-10 in the liver(P<0. 01). In conclusion, Zhuyu Pills demonstrate definite therapeutic effects on aortic plaques and seborrhea in mice and can alleviate inflammatory responses by regulating the CD1d/iNKT cell axis-mediated Th1/Th2 balance. - Source: PubMed
Song WeiTeng Zheng-WenZhang Zhong-YiDang Si-JieShen TaoXiao MinZhang Yong - Vγ9Vδ2-T cells form a conserved T-cell subset known for its potent intrinsic antitumor activity and versatility in recognizing diverse cancer types independently of the major histocompatibility complex. Previously, we reported the preclinical activity of a bispecific T-cell engager (bsTCE) specific for both CD1d and the Vδ2-TCR that engaged both Vγ9Vδ2-T and type 1 natural killer T cells to CD1d-expressing hematological malignancies, including multiple myeloma (MM) and acute myeloid leukemia (AML). Here, we evaluated whether various standard-of-care drugs for patients with MM and AML/myelodysplastic syndromes (MDS) affected the in vitro antitumor activity of CD1d-Vδ2 bsTCE-activated Vγ9Vδ2-T cells. - Source: PubMed
Publication date: 2026/06/28
de Jong MilonVeth MyrtheBrachtlová TerezaKing Lisa ASaura-Esteller JoséBechler Tamara Mvan Helden Pauline MAlhan CananLameris Roelandde Gruijl Tanja Dvan der Vliet Hans J - CD1d-restricted invariant natural killer (iNK) T cells are innate T cells known for their ability to shape adaptive immunity toward inflammation or immune-suppression via the rapid production of Th1-, Th2-, and Th17-type cytokines from corresponding iNKT subsets such as NKT1, NKT2, and NKT17. IL-10-producing invariant NKT cells, termed NKT10 cells, are thought to play an immunoregulatory role, but their potential clinical use remains underexplored. We characterized human NKT10 cells from cord-derived iNKT cells and investigated their therapeutic utility in allogeneic stem cell transplantation. Cord and cord-derived iNKT cells contained a high frequency of CD4CD25CD161FoxP3 iNKT cells and showed Th2/Th10-biased cytokine production upon antigenic stimulation. Accordingly, cord-derived iNKT cells displayed a distinct gene expression profile with upregulated genes related to NKT2, NKT10, and regulatory T cells compared with adult donor-derived iNKT cells. Furthermore, single-cell RNA sequencing analysis of cord-derived iNKT cells confirmed the presence of NKT10-like subset that was enriched with multiple immunoregulatory pathways and genes related to immune-checkpoints (, , , and ) and NKT10 (, , and ), whereas the NKT1/17-like subset present in adult donor-derived iNKT cells showed upregulation of genes related to cytotoxicity (, , and ), NKR (, , , and ), NKT1 ( and ), and NKT17 (). Lastly, cord-derived iNKT cells suppressed alloreactive T cell proliferation and ameliorated xenogeneic graft-versus-host disease where the immunodeficient NSG mice received human peripheral blood mononuclear cells supplemented with cord-derived iNKT cells. Thus, NKT10-enriched, cord-derived iNKT cells are candidate cell therapeutics for immune-modulation in allogeneic stem cell transplantation and other autoimmune diseases. - Source: PubMed
Publication date: 2026/06/10
Trujillo-Ocampo AbelBorges PamellaGrefe MaisonVaz de Freitas MartielaLee Sung-EunQi YuanClinton JelitaLi DanHe HongYu LingPeris-Cuesta ArnauEhli Erik AMa QingSu XiaopingAmaral Antunes DinlerAl-Atrash GheathMolldrem Jeffrey JShpall Elizabeth JIm Jin S - Nasopharyngeal carcinoma (NPC) is a subtype of head and neck squamous cell carcinoma characterized by high recurrence and metastasis rates and poor prognosis. Although immune checkpoint inhibitors have emerged as a promising treatment strategy for recurrent/metastatic nasopharyngeal carcinoma (R/M NPC), only a few patients have benefitted significantly from them. Lipid metabolism reprogramming plays a crucial role in NPC progression and its interaction with the immune microenvironment. This study aims to establish a prognostic model for NPC based on lipid metabolism-related factors, further explore its association with tumor immunity, and investigate the potential for immunotherapy. - Source: PubMed
Publication date: 2026/06/25
Xu YangZhu LiruZhang QingqingChen XiaojunLv HaoyuMa ShuhanWang RunzhiChang YuanyuanSun YongchuChen KaihuaLi LingZhu Xiaodong - Objective To investigate the early transcriptomic characteristics of neutrophils and monocytes stimulated by the major surface protein 2 (MSP2) of anaplasma phagocytophilum(AP), to preliminarily explore their functional differences and potential common pro-inflammatory mechanisms of these two cell types in the inflammatory response, and to provide a theoretical basis for understanding the inflammatory pathogenesis of Human Granulocytic Anaplasmosis (HGA) and for developing anti-inflammatory strategies. Methods HL60 cells (rHL60) differentiated with dimethyl sulfoxide (DMSO) for 8 days were used as a neutrophil-like cell model, and human acute monocytic leukemia (THP-1) cells were used as a monocyte model. Both cells were stimulated with recombinant MSP2 (rMSP2) for 2 hours. Differentially expressed genes (DEGs) were identified by transcriptome sequencing, and their biological functions and related signaling pathways were analyzed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. The mRNA expression levels of key DEGs were validated by real-time quantitative PCR (qPCR), and the concentrations of cytokines in cell supernatants were measured by ELISA. Results Transcriptome analysis showed that in rHL60 cells, DEGs were mainly enriched in inflammation. qPCR confirmed the up-regulation of C-C motif chemokine receptor 7 (CCR7) and oxidized low-density lipoprotein receptor 1 (OLR1), while CD1D molecule and C-X-C motif chemokine receptors 1/2 (CXCR1/2) were down-regulated. In THP-1 cells, DEGs were primarily enriched in transcriptional regulation. qPCR validated the up-regulation of nuclear factor kappa B inhibitor zeta (NFKBIZ) and nuclear receptor subfamily 4 group A member 3 (NR4A3), whereas thioredoxin-interacting protein (TXNIP) and interleukin-16 (IL-16) were down-regulated. ELISA results demonstrated that both cell types significantly secreted C-C motif chemokine ligands 3/4/20 (CCL3/4/20) following MSP2 stimulation. Conclusion In the early phase of MSP2 stimulation, neutrophils serve as the primary cells initiating the inflammatory response, whereas monocytes focus on immune regulation. The co-secretion of CCL3, CCL4, and CCL20 may represent a potential molecular mechanism by which these two innate immune cell types jointly promote inflammatory responses during AP infection. - Source: PubMed
Chen ShihuaWang FengYue LeiYan Min