Anti-Mouse CD3e FITC 50 ug
- Known as:
- Antibody toMouse CD3e fluorecein 50 ug
- Catalog number:
- 11-0031-81
- Category:
- -
- Supplier:
- eBioscience
- Gene target:
- Anti-Mouse CD3e FITC 50
Related genes to: Anti-Mouse CD3e FITC 50 ug
- Gene:
- CD3E NIH gene
- Name:
- CD3e molecule
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 11q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: Anti-Mouse CD3e FITC 50 ug
Related articles to: Anti-Mouse CD3e FITC 50 ug
- Spatial immune cell profiling using multiplex immunofluorescence was examined to characterize the inflammatory cell infiltrates and to possibly aid in the distinction of acute cellular rejection (ACR) and lymphocytic bronchiolitis (LB) from its mimickers including bronchus-associated lymphoid tissue (BALT) and infection in lung allograft tissue. Lung allograft biopsies with ACR and/or LB were identified from an institutional pathology lung transplant database (2012-2024). Surgical lung specimens, transbronchial biopsies, and autopsy lungs were used for BALT and infection, respectively. Multiplex immunofluorescence using the PhenoCode Signature Immune Profile Human Protein Panel (CD3e, CD8, CD20, CD68, FoxP3, pankeratin) was performed. The study included tissue specimens with ACR (n = 13), LB (n = 10), BALT (n = 12), and infection (n = 13). The percentage of CD3 + T-cells, CD8 + T-cells, and CD68 + macrophages was higher and the percentage of CD20 + B-cells was lower in ACR, LB, and infection than BALT (p ≤ 0.02, all). The percentage of FoxP3 + CD3 + T-cells was higher in ACR and BALT than infection (p ≤ 0.02, all). In conclusion, ACR and LB are predominantly comprised of T-cells, however B-cells, macrophages, and FoxP3 + CD3 + T-cells are also present. The immune cell composition of ACR and LB is distinct from that of BALT but similar to infection. - Source: PubMed
Publication date: 2026/08/31
Klaisuban WipawiKroneman Trynda NFlotte Thomas JJenkins Sarah MKopp Karla JAubry Marie-ChristineHsu Ying-Han RSaddoughi Sahar AScott John PRoden Anja C - Antigen 85B (Ag85B), an immunodominant protein secreted by (Mtb), induces robust T-cell-mediated immunity in host cells. Previous studies have demonstrated that recombinant BCG strains overexpressing Ag85B (BCG85B) confer significant protection against tuberculosis in mouse models. This study hypothesized that enhanced protection by recombinant BCG85B results from modulation of host immune gene expression by the Ag85B antigen. To test this, RNA-seq analysis was performed on splenocytes from mice immunized with the wild-type BCG (BCG-WT) and BCG85B strains. Differentially expressed genes (DEGs) in the splenocytes of BCG85B-immunized mice indicated upregulation of immune-related KEGG pathways, including Th1/Th2 and Th17 differentiation, NF-kappa B signaling, and cytokine-cytokine receptor interactions, as well as Gene Ontology (GO) processes such as immune response activation, α-β T cell activation, T-cell production and differentiation, IL-15 mediated signaling, and cytokine-mediated signaling pathways and down regulation of metabolic and digestive processes. Additionally, transcription factor (TF) and protein-protein interaction (PPI) analyses confirmed upregulation of genes involved in T cell receptor signaling and immune activation (, , , , , , and ) in splenocytes from BCG85B-immunized mice. Collectively, these findings indicate that Ag85B overexpression enhances adaptive immune activation and reprograms cellular metabolic pathways, thereby increasing vaccine efficacy. - Source: PubMed
Publication date: 2026/08/12
Chauhan VivekKumar RakeshVeerapandian RajaRamos Enrique IJagannath ChinnaswamyGadad Shrikanth SDhandayuthapani Subramanian - BACKGROUNDElucidating immune signals through well-defined cohorts of pediatric acute pancreatitis (AP) and chronic pancreatitis (CP) patients is critical. This study aimed to evaluate plasma chemokine and cytokine levels in pediatric participants with CP, compared with AP and healthy controls (HCs), to identify unique biomarkers of CP.METHODSIndividuals were identified from a prospectively collected pediatric cohort (n = 146). Immunoproteins (n = 247) were measured using the NULISAseq platform on samples from individuals with CP (n = 71), AP (n = 55), and HCs (n = 20).RESULTSThe measured analytes showed separation among the 3 groups (R2 = 0.13, P < 0.001). In the CP group, TRANCE, TWEAK, FLT-1, HGF, and TRAIL were increased when compared with HC and AP patients (FDR-corrected P <0.05). A multivariable logistic regression model including all 5 proteins provided an AUC of 0.94 (0.93-0.96) for differentiating samples from CP versus AP or HC samples. In the AP group, CRP, IL-6, CD3E, ENRAGE, and MIF were elevated compared with HCs, while FGF-2, TAFA-5, IL-33, TRANCE, and CXCL12 were downregulated in the same acute time period (FDR-corrected P < 0.05). In the 21 patients with AP for whom follow-up samples were obtained, there was a notable decrease in sequential expression of IL-6 and CRP over 12 months and increased expression of CCL25, TAFA-5, and TRANCE proteins. Additionally, TRANCE was expressed on CP pancreatic tissue.CONCLUSIONSTRANCE was increased in pediatric patients with CP and decreased in those with AP during a flare. Future studies are needed to investigate the role of TRANCE and other analytes in the pathogenesis of CP. - Source: PubMed
Publication date: 2026/08/24
Farrell Peter RLee BomiAhmed FaizanMoreno-Fernandez Maria EDixit AjayGurria Juan PabloOllberding Nicholas JDuan QingGarlapally VineetChristian PhoebeHusain Sohail ZAbu-El-Haija Maisam - Banff acute lesion scores underpin histologic classification of kidney allograft biopsies; however, biomarker studies rely on second-order associations with diagnostic categories that introduce confounding. We quantified the first-order relationships between Banff acute lesion scores and the validated urinary cell three-gene rejection signature. In 354 biopsy-urine pairs, three-gene signature scores computed using a locked regression equation incorporating absolute copy numbers of CD3E mRNA, CXCL10 mRNA, and 18S rRNA in urinary cell RNA-were related to glomerulitis ( ), peritubular capillaritis ( ), interstitial inflammation ( ), and tubulitis ( ). Signature scores rose monotonically with Banff acute lesion severity, with 1.5 to 1.8-fold higher odds of more severed and (all P<0.0001), and showed good calibration. Associations remained robust for composite microvascular ( ) and tubulointerstitial ( ) indices and were strongest for severe and , supporting this signature as a noninvasive, quantitative readout of acute rejection pathology with immediate diagnostic applicability. - Source: PubMed
Publication date: 2026/07/27
Li CarolSchwartz Joseph ESalinas ThaliaDadhania Darshana MDeVito AlexHiggins WilliamSalvatore StevenSeshan Surya VSharma Vijay KMuthukumar ThangamaniSuthanthiran Manikkam - Sepsis is a systemic inflammatory response caused by pathogen infection, leading tomultiple organ failure. This study aims to identify potential sepsis biomarkers. - Source: PubMed
Publication date: 2026/07/30
Shen XingkaiGuo JianYang KailiJin Yuhong