Lax1 siRNA_Lentivectors
- Known as:
- Lax1 siRNA_Lentivectors
- Catalog number:
- i064316b
- Product Quantity:
- 500ng
- Category:
- -
- Supplier:
- ABM
- Gene target:
- Lax1 siRNA_Lentivectors
Related genes to: Lax1 siRNA_Lentivectors
- Gene:
- LAX1 NIH gene
- Name:
- lymphocyte transmembrane adaptor 1
- Previous symbol:
- -
- Synonyms:
- LAX, FLJ20340
- Chromosome:
- 1q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 2005-04-25
- Date modifiied:
- 2014-11-19
Related products to: Lax1 siRNA_Lentivectors
Related articles to: Lax1 siRNA_Lentivectors
- The development of axillary meristems (AMs) is pivotal for tillering and panicle branching in rice, processes that ultimately determine grain yield. LAX PANICLE1 (LAX1) encodes a basic helix-loop-helix (bHLH) transcription factor essential for AM formation during panicle development. However, because LAX1 possesses a non-canonical basic region with fewer than five basic amino acids, it has long been presumed to lack intrinsic DNA-binding capacity, leaving its molecular mechanism of action unclear. Here, we demonstrate that LAX1 localizes to the nucleus and exhibits transcriptional activation activity. Using Systematic Evolution of Ligands by EXponential enrichment (SELEX) assays, we revealed that LAX1 possesses intrinsic DNA-binding capability in vitro. Furthermore, chromatin immunoprecipitation sequencing (ChIP-seq) analysis of young rice panicles identified direct LAX1 target genes, with binding peaks significantly enriched for a specific motif, TTTTGC(T/A/C)NNN(G/A/T), which is directly bound by the LAX1 bHLH domain. Subsequent experiments validated that LAX1 directly binds the promoters of OsPID, OsIAA7, LOG, and OsTPS8. Molecular and genetic analyses provided evidence supporting the functional role of the LAX1-OsPID module in regulating inflorescence architecture. Comparative transcriptome analysis of lax1 mutants and wild-type plants further indicated that LAX1 participates in multiple biological processes. Interestingly, we found that LAX1 and its orthologs likely regulate downstream gene expression by recognizing a common set of cis-regulatory elements across diverse cereal crops. Collectively, our findings redefine LAX1 and its orthologs as canonical bHLH transcription factors, elucidate their regulatory network in controlling panicle branching, and provide a theoretical foundation for the cross-species application of LAX1 in crop yield improvement. - Source: PubMed
Publication date: 2026/05/25
Fu DebaoXiong XiaohuWei MinghuaGao TianXu TingtingZhu JunkaiMa ShuaiguoZhu ChunmeiKong QiushengWu Changyin - Drought is a major constraint to rice ( L.) yield. The young spike differentiation stage is crucial for yield determination, exhibiting heightened sensitivity to soil moisture deficits. (), a -like gene, is a key regulator of young spike differentiation and may mediate drought-induced delays in floral transition in rice. This study examines the effect of drought stress during the young spike differentiation stage on rice spike structure and investigates the role of in this process. - Source: PubMed
Publication date: 2026/04/13
Feng XinyiZhong XintongWen JiaminLiu YuWang YanZha Manrong - Pancreatic cancer remains highly lethal, largely due to late diagnosis and limited efficacy of treatments. Improving first-line treatment selection and patient monitoring requires novel, non-invasive biomarkers beyond carbohydrate antigen 19-9 (CA19-9) and imaging. This study investigates epigenetic biomarkers from liquid biopsy with prognostic and predictive potential in metastatic pancreatic ductal adenocarcinoma (PDAC; mPDAC). Genome-wide methylation profiling of cell-free DNA (cfDNA) from healthy individuals and stage IV mPDAC patients identified 13 gene-associated CpG sites with significantly altered methylation patterns. ddPCR validation confirmed consistent methylation differences in lymphocyte transmembrane adaptor 1 (), nuclear receptor subfamily 3 group C member 1 (), and between healthy and patient groups. Elevated and methylation and reduced methylation at diagnosis were associated with poor prognosis and correlated with high-risk circulating biomarker profiles, including CA19-9 levels, MAF (mutant allele fraction), cfDNA concentration, and cfDNA fragmentation. Notably, baseline methylation levels predicted response to first-line FOLFIRINOX-based treatment with an acceptable 75% sensitivity and a high specificity of 92.86%. These findings highlight the clinical significance of cfDNA methylation as a minimally invasive biomarker source, emphasizing , , and as prognostic biomarkers in mPDAC. Specifically, baseline methylation emerges as a promising predictor of treatment response, supporting personalized therapeutic strategies in mPDAC. - Source: PubMed
Publication date: 2026/03/22
Cano-Ramírez PabloToledano-Fonseca MartaCano-Osuna María TeresaHerrera-Casanova NereaCarrillo-Pecero EmilioRodríguez-Ariza AntonioAranda EnriqueGarcía-Ortiz María Victoria - Genetic transformation of elite crop varieties remains limited by genotype-specific recalcitrance and dependence on tissue culture. This review provides a comprehensive analysis of current transformation platforms and emerging strategies to overcome these bottlenecks. We examine traditional -mediated and biolistic methods, then critically assess tissue culture-free approaches including floral-based delivery, cut-dip-budding, injection, and viral vector-mediated transformation systems. A major focus is the deployment of developmental regulators-, chimeras, transcription factors, , , and -that enhance regeneration efficiency across genotypes. We detail their molecular mechanisms, from chromatin remodeling and auxin gradient establishment to wound-responsive cell reprogramming. Importantly, we address the pleiotropic developmental costs of DR misexpression and review precision control technologies, including promoter optimization and auto-excision systems, that enable transient DRs activity during regeneration while ensuring normal plant development. We propose a roadmap for integrating these advances to achieve genotype-flexible, high-throughput transformation applicable to molecular design breeding.(1). - Source: PubMed
Publication date: 2026/03/11
Guo YajieLi MengyaoLiu MengtianLiu Huiyun - Efficient in vitro regeneration remains a major constraint in the genetic transformation, genome editing, and molecular breeding of wheat ( L.), largely due to strong genotype-dependent recalcitrance and limited activation of developmental programs required for somatic embryogenesis. Plant regeneration relies on extensive transcriptional reprogramming and epigenetic remodeling orchestrated by morphogenic regulators that modulate meristem identity, as well as cellular pluri- and totipotency. In this review, we synthesize current molecular knowledge on key transcription factors (, , , , , , ) and signaling peptides ( module, phytosulfokine/) that regulate embryogenic competence in monocot cereals, with emphasis on their orthologs and functional relevance in wheat. We highlight how controlled expression of these morphogenic genes, promoter engineering, and transient or excisable induction systems can significantly enhance regeneration capacity, reduce chimerism in CRISPR-Cas-edited plants, and facilitate genotype-independent transformation. We also discuss epigenetic and metabolic constraints underlying wheat recalcitrance and their potential modulation to improve culture responsiveness. By integrating evidence from wheat, rice, maize, and barley, we outline conserved gene-regulatory networks that reinitiate totipotency and propose strategies to accelerate doubled haploid production and speed-breeding pipelines. Collectively, morphogenic factors emerge as central molecular tools for overcoming regeneration bottlenecks and enabling next-generation wheat improvement. The objective of this review is to synthesize and critically evaluate current molecular knowledge on morphogenic regulators controlling in vitro regeneration in wheat ( L.), with particular emphasis on their roles in genetic transformation and genome editing. - Source: PubMed
Publication date: 2026/01/27
Kowalik SylwiaSamoń MonikaPrzyborowski Mateusz