Vps37a siRNA_Lentivectors
- Known as:
- Vps37a siRNA_Lentivectors
- Catalog number:
- i063181c
- Product Quantity:
- 500ng
- Category:
- -
- Supplier:
- ABM
- Gene target:
- Vps37a siRNA_Lentivectors
Related genes to: Vps37a siRNA_Lentivectors
- Gene:
- VPS37A NIH gene
- Name:
- VPS37A subunit of ESCRT-I
- Previous symbol:
- PQBP2
- Synonyms:
- FLJ32642, HCRP1, SPG53
- Chromosome:
- 8p22
- Locus Type:
- gene with protein product
- Date approved:
- 2005-09-08
- Date modifiied:
- 2019-01-31
Related products to: Vps37a siRNA_Lentivectors
Related articles to: Vps37a siRNA_Lentivectors
- Antibodies are known to prevent infection by binding to pathogens extracellularly and blocking their entry into cells. What is less well known is that a proportion of pathogens, called the persistent fraction, still succeed in entering cells despite the antibodies bound to their surface. Fortunately, all mammalian cells express a dedicated cytosolic antibody receptor called TRIM21 that intercepts these incoming antibody-bound pathogens as soon as they enter the cytosol. Once it has detected an infection event, TRIM21 uses its E3 ubiquitin ligase activity to target pathogens for degradation. Our early work showed that TRIM21-mediated neutralization was both a fast and efficient process, capable of causing the degradation of incoming viral particles within hours. What was less clear was how TRIM21 achieves this degradation. In a recent study, we reveal that TRIM21 mediates a system of selective autophagy to direct incoming pathogens into the lysosome.: TRIM21:Tripartite-motif containing protein 21; VCP: Valosin-Containing Protein. CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats; FACS: fluorescence activated cell sorting; GFP: green fluorescent protein; LC3: Microtubule-associated Protein 1 Light Chain 3; TBK1: TANK-binding kinase 1; FIP200: FAK family kinase-interacting protein of 200 kDa; ULK1: Unc-51-like autophagy activating kinase 1; PI3K: Phosphoinositide 3-Kinase; ATG: autophagy-related gene; TMEM41B: transmembrane protein 41B; VPS37A: Vacuolar Protein Sorting-Associated Protein 37A; RBSN: Rabenosyn-5; EPG5: Ectopic P-Granules 5 Autophagy Tethering Factor; NDP52: Nuclear Dot Protein 52; ADX: antibody-dependent xenophagy; p62/SQSTM1: Protein 62/ Sequestosome 1; LPS: Lipopolysaccharide. - Source: PubMed
Publication date: 2026/07/22
Rhinesmith TAlbecka AJames L C - VPS37A, a subunit of ESCRT-I involved in endosomal sorting and autophagy, is frequently downregulated in diverse human cancers. In this study, we showed that VPS37A downregulation, as part of a large chromosome 8p deletion, arises early during tumorigenesis and persists throughout tumor progression. Integrative analysis of VPS37A gene copy number and CRISPR dependency revealed that VPS37A deficiency creates a synthetic lethal dependency on the MAP3K7-NF-κB-CFLAR axis, and targeting this axis triggered CASP8-mediated apoptosis and suppressed tumor growth. This synthetic vulnerability depends on ATG8ylated membranes, which serve as a platform for CASP8 activation upon inhibition of phagophore closure, and can be triggered without disrupting receptor sorting. Consistently, despite frequent co-deletion of VPS37A and the death receptors TNFRSF10A/B, inhibition of the MAP3K7-NF-κB-CFLAR axis selectively induced apoptosis in spheroid tumors with 8p deletion. These results uncover a selective vulnerability in cancer cells harboring VPS37A/8p loss, providing a mechanistic rationale for targeted therapeutic intervention. - Source: PubMed
Publication date: 2026/07/07
Hattori TatsuyaChen LongguiLiang XinwenHamamoto KoutaWang Hong-GangTakahashi Yoshinori - Growing evidence highlights the critical involvement of the ubiquitin-proteasome system in cancer development. As an essential component of the 26 S proteasome, Proteasome 26 S Subunit ATPase 2 (PSMC2) has been implicated in various malignancies, but its role in hepatocellular carcinoma (HCC) progression remains poorly understood. We analyzed PSMC2 expression using The Cancer Genome Atlas database (TCGA) database, and validated findings in clinical HCC specimens and cell lines through immunohistochemistry (IHC) and Western blot. Functional assays (CCK-8, colony formation, Scratch test and transwell invasion assay) were performed to assess the oncogenic properties of PSMC2 in the progression of HCC. Mechanistic studies employed co-immunoprecipitation, Western blot, immunofluorescence and in vivo xenograft models to investigate PSMC2-EGFR interactions and downstream signaling. PSMC2 was significantly overexpressed in HCC tissues and correlated with poor patient prognosis. Genetic knockdown of PSMC2 inhibited HCC cell proliferation, migration, and invasion in vitro, while suppressing tumor growth in vivo. Conversely, PSMC2 overexpression enhanced malignant phenotypes. Mechanistically, PSMC2 physically interacted with EGFR, stabilizing EGFR protein levels and enhancing phosphorylation of downstream AKT and ERK1/2 pathways. Our study identifies PSMC2 as a novel regulator of HCC progression through EGFR-AKT/ERK1/2 signaling axis activation. These findings position PSMC2 as both a prognostic biomarker and a potential therapeutic target for HCC intervention. - Source: PubMed
Publication date: 2026/05/24
Li YechengSu QunxueFeng ZhenyuXu HuiHe Tengfei - In order to investigate the basic genetic structure of plumage colour in Jingyuan chicken and to explore the genetic markers for plumage colour development, the present study was carried out to investigate the candidate key SNPs and candidate genes regulating black, linen and white plumage and plumage traits of Jingyuan chicken by using selection signal analysis and genome-wide association analysis. Selection signal analyses showed that including 30, 40 and 18 overlapping genes were associated with black, linen and white plumage colours in Jingyuan chicken. Meanwhile, integrative genomic analyses identified BCAT1, LMO3, and PIK3C2G as primary candidates for black plumage, and IL1RAPL1 for white plumage, with all genes showing convergent support across multiple complementary approaches. Further screening through Fst and θπ values identified 10 key candidate genes significantly associated with plumage colour traits, including ARFGEF2, VPS37A, SNX12, KIT, MET, ROS1, CSF1R, NGF, CDC42, and TEK. These results provide a fundamental theoretical basis for the genetic structure and formation of Jingyuan chicken plumage. - Source: PubMed
Publication date: 2025/08/06
Yang LijuanZhao WeiChen SiyuXue LinTian JinliXu HairongZhang HaiboWang HuaGu YalingZhang Juan - VPS37A (VPS37A subunit of ESCRT-I), a component of the ESCRT-I (endosomal sorting complex required for transport I) complex, mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Although accumulating evidence indicates that VPS37A deficiency occurs in numerous malignancies and exerts tumor-suppressive effects during cancer progression, its functional significance in colorectal cancer (CRC) pathogenesis remains poorly characterized. Therefore, this study aims to further investigate the functional and molecular mechanisms by which VPS37A downregulation contributes to malignant biological phenotypes in CRC, with a specific focus on how its dysregulation affects cell death pathways. - Source: PubMed
Publication date: 2025/07/18
Liu ChunchengLiu XiaohanLi ZiqiWei YanruoxueLiu BangdongZhu PengLiu YukunZhao Ran