RHCE Antibody
- Known as:
- RHCE Antibody
- Catalog number:
- 40210
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Signalway
- Gene target:
- RHCE Antibody
Related genes to: RHCE Antibody
- Gene:
- RHCE NIH gene
- Name:
- Rh blood group CcEe antigens
- Previous symbol:
- RH
- Synonyms:
- CD240CE
- Chromosome:
- 1p36.11
- Locus Type:
- gene with protein product
- Date approved:
- 1993-10-22
- Date modifiied:
- 2019-04-23
Related products to: RHCE Antibody
Related articles to: RHCE Antibody
- The Rh blood group system is the most polymorphic and clinically significant blood group system after ABO. The RhD protein, in particular, encompasses numerous conformation-dependent epitopes that can elicit alloimmune responses, leading to hemolytic disease of the fetus and newborn or hemolytic transfusion reactions. Despite this, traditional serologic methods for RhD typing lack sensitivity and specificity to detect RhD variants like partial D and DEL phenotypes. This chapter describes molecular methods to characterize RhD mRNA expression and identify transcript variants in immortalized leukemic or erythroid cell lines and primary reticulocytes. Conventional PCR and quantitative real-time PCR assays were developed using primers designed against the full-length transcripts of RhD and RhCE and known splicing isoforms of RhD. The relative abundance of RhD isoforms in K562 cells was determined from public RNA-seq databases such as Cancer Cell Line Encyclopedia to guide primer design and template input amounts. The methods detail RNA extraction, cDNA synthesis, PCR amplification, and quantitative real-time PCR quantification protocols, with representative results shown for multiple cell lines that are widely used in research settings. To enable unbiased discovery of novel RhD transcript isoforms, a Nanopore long-read cDNA sequencing approach was established. As an orthogonal method, the flow cytometry assay was optimized to detect the RhD full-length protein on the cell surface. These molecular and cellular approaches complement serologic methods and support the transition to genomics-based approaches for Rh typing. Transcriptome-level data, supported by protein-level validation, can provide insights into the regulation of RhD expression and the functional impact of variants. These protocols can be readily applied to erythroid cell lines for mechanistic studies and extended to other blood group genes. - Source: PubMed
Liu JunGuan XiaoyuWang JueShen ZiyangChang DanielLi LingLiu ZhongChai Li - From January 2022 to June 2023, this study enrolled 124 healthy blood donors. Column agglutination and third-generation sequencing (TGS) were used for genotyping and RhCE antigen typing. Additional serological screening was performed using conventional tube and column agglutination methods. Sanger sequencing was performed on 22 specimens with abnormal RhCE expression. Among the 124 samples, 102 exhibited normal RhCE expression whereas 22 showed abnormal expression; TGS-predicted phenotypes disagreed with serological results in eight of the 102 normal-expression samples and in seven of the 22 abnormal-expression samples, with discrepancies attributed to reagent limitations and the presence of novel alleles. Indeed, six novel alleles were identified by TGS and their potential phenotypes inferred based on serological findings, demonstrating that conventional tube and column agglutination methods are effective in determining serological phenotypes, especially in cases of weak RhCE antigen expression, whereas TGS proves valuable for genotyping and elucidating the underlying causes of weakened expression. - Source: PubMed
Publication date: 2026/08/21
Wu FanSun Li-YanLiang Shuang - While the previous review encompassed the Rh blood group system (Chou ST, Westhoff CM. The Rh and RhAG blood group systems. . 2010;26:178-86), this update focusses on the gene and its variants. Four new antigens- PARG, CEVF, CEWA, and CETW (RH60 to RH63)-were reported since the last update. ) was amended from a null allele to an allele encoding very weak antigen expression. The following topics are discussed: cross-reactive alleles [such as (*) and * (*) which may type D+ with some monoclonal anti-D reagents], issues with hybrid alleles and allele dropout, common haplotypes (association between alleles and specific alleles), and clinical considerations. While the detailed description of new Rh antigens has become rare, many alleles have been reported since the previous review, a result of increased adoption of DNAbased testing for red blood cell antigens in immunohematology laboratories. The Rh blood group system has fascinated generations of immunohematologists and is likely to continue to do so for decades to come. - Source: PubMed
Publication date: 2026/07/23
Floch AlineTournamille ChristopheVege Sunitha - The D- - phenotype is an exceptionally rare Rh configuration characterised by the expression of the D antigen but absence of all RHCE-encoded antigens. A few molecularly defined cases have been documented globally. - Source: PubMed
Publication date: 2026/07/03
Madkhali Maymoon MMeshi Abdullah AOsman MahdiAlnefaie AbdulrahmanAlmalky HosainKhormy MayisahGhazwani KhaledHamali Hassan AStef Marianne - This study aimed to validate the efficacy of right-heart contrast echocardiography (RHCE) combined with the Valsalva manoeuvre for screening for patent foramen ovale (PFO) in patients with migraine without an identified secondary cause. - Source: PubMed
Gu HaijuanXia JieshengXu ZhenhuaShi Jianwei