ELF1 antibody - middle region (P100872_P050)
- Known as:
- ELF1 (anti-) - middle region (P100872_P050)
- Catalog number:
- p100872_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- ELF1 antibody - middle region (P100872_P050)
Related genes to: ELF1 antibody - middle region (P100872_P050)
- Gene:
- ELF1 NIH gene
- Name:
- E74 like ETS transcription factor 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 13q14.11
- Locus Type:
- gene with protein product
- Date approved:
- 1995-03-23
- Date modifiied:
- 2016-10-05
- Gene:
- ELOF1 NIH gene
- Name:
- elongation factor 1 homolog
- Previous symbol:
- -
- Synonyms:
- MGC4549, ELF1
- Chromosome:
- 19p13.2
- Locus Type:
- gene with protein product
- Date approved:
- 2005-03-25
- Date modifiied:
- 2016-02-10
Related products to: ELF1 antibody - middle region (P100872_P050)
Related articles to: ELF1 antibody - middle region (P100872_P050)
- Transcription-coupled nucleotide excision repair (TC-NER) is a highly conserved DNA repair pathway that removes bulky lesions in the transcribed genome. Cockayne syndrome B protein (CSB), or its yeast ortholog Rad26, has been known for decades to play important roles in the lesion-recognition steps of TC-NER. Another conserved protein ELOF1, or its yeast ortholog Elf1, was recently identified as a core transcription-coupled repair factor. How Rad26 distinguishes between RNA polymerase II (Pol II) stalled at a DNA lesion or other obstacles and what role Elf1 plays in this process remains unknown. Here, we present cryo-EM structures of Pol II-Rad26 complexes stalled at different obstacles that show that Rad26 uses a common mechanism to recognize a stalled Pol II, with additional interactions when Pol II is arrested at a lesion. A cryo-EM structure of lesion-arrested Pol II-Rad26 bound to Elf1 revealed that Elf1 induces further interactions between Rad26 and a lesion-arrested Pol II. Biochemical and genetic data support the importance of the interplay between Elf1 and Rad26 in TC-NER initiation. Together, our results provide important mechanistic insights into how two conserved transcription-coupled repair factors, Rad26/CSB and Elf1/ELOF1, work together at the initial lesion recognition steps of transcription-coupled repair. - Source: PubMed
Publication date: 2024/01/09
Sarsam Reta DXu JunLahiri IndrajitGong WenzhiLi QingrongOh JuntaekZhou ZhenHou PeiniChong JennyHao NanLi ShishengWang DongLeschziner Andres E - Despite having been sequenced over a decade ago, the functional significance of much of the mammalian genome remains unknown. The mouse has become the preeminent mammalian model for identifying endogenous gene function in vivo. Here we characterize the phenotype of a loss-of function allele for the evolutionarily conserved transcription factor, Elongation Factor Homolog 1 (Elof1). Recent work utilizing the yeast homolog, Elf1, has demonstrated that Elf1 associates with the RNA polymerase II complex to promote elongation by relieving the association of the template DNA strand with bound histones. Loss of Elof1 results in developmental delay and morphological defects during early mouse development resulting in peri-gastrulation lethality. Although Elof1 is highly conserved we observe tissue specific expression during gastrulation and in adult murine tissues, suggesting there may be other genes with similar function in diverse tissues or that mElof1 has adopted lineage specific functions. To better understand its function in mammalian transcription, we examined splice variants and find that Elof1 regulates mutually exclusive exon use in vivo. Distinct from what has been demonstrated in yeast, we demonstrate that Elof1 is essential for viability during mammalian gastrulation which may be due to a role mediating tissue specific exclusive exon use, a regulatory function unique to higher eukaryotes. - Source: PubMed
Publication date: 2019/07/05
Tellier Adam PArchambault DanielleTremblay Kimberly DMager Jesse