TCF12 antibody - N-terminal region (P100771_P050)
- Known as:
- TCF12 (anti-) - N-terminal region (P100771_P050)
- Catalog number:
- p100771_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- TCF12 antibody - N-terminal region (P100771_P050)
Related genes to: TCF12 antibody - N-terminal region (P100771_P050)
- Gene:
- TCF12 NIH gene
- Name:
- transcription factor 12
- Previous symbol:
- -
- Synonyms:
- HEB, HTF4, HsT17266, bHLHb20, p64
- Chromosome:
- 15q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-06-17
- Date modifiied:
- 2018-02-20
Related products to: TCF12 antibody - N-terminal region (P100771_P050)
Related articles to: TCF12 antibody - N-terminal region (P100771_P050)
- Recently, a distinct, bland spindle cell neoplasm with rhabdomyoblastic phenotype, and VGLL3 rearrangement has been described. These tumors have a striking predilection for the head and neck area and so far, followed an indolent course. It remains unclear whether these tumors are best classified as true rhabdomyosarcomas. There are 11 reports of such tumors with limited follow-up. Here, we report an additional case with local recurrence, long-term follow-up and spatial profiling. The tumor occurred in the right buccal mucosa/oral commissure of a 47-year-old man. On clinical examination, the mass was firm, measuring ~1.5 cm. Biopsy and subsequent wedge excision were performed. Histologically, the tumor was composed of bland, small, spindle to ovoid cells, arranged in short fascicles and vaguely storiform architecture. The tumor cells diffusely infiltrated into skeletal muscle. There was a background of inflammatory cells including small lymphocytes and histiocytes. Neoplastic cells were positive for SMA, demsin, PAX7, myogenin and MyoD1. Whole transcriptome sequencing revealed a TCF12::VGLL3 fusion. Digital spatial profiling (DSP) identified pan-AKT expression, differential expression in the MAPK pathway, and revealed that the tumor attracted a dense T-cell rich inflammatory infiltrate. The patient had a lesion in the same location 6 years prior that underwent incisional biopsy, showed intense inflammatory infiltrate, and was interpreted as benign. FISH for VGLL3 on this tissue was positive for rearrangement. No additional adjuvant treatment was given, and the patient is alive without disease, 8 months after the major resection. Long term follow-up of 6 years with only local recurrence lends further support to the notion that these neoplasms are a class of indolent/low-grade rhabdomyoblastic tumors that are biologically and clinically distinct from fully malignant spindle cell rhabdomyosarcomas. - Source: PubMed
Dashti Nooshin KChakraborty DebopriyaSadanandappa Madhumala KDehner Carina AZhang Paul JPaydarfar Joseph ATafe Laura J - The glioma tumor microenvironment (TME) exhibits profound heterogeneity that drives tumor progression and therapy resistance. By integrating single-cell RNA sequencing (eleven samples) and spatial transcriptomics (two samples), the cellular components of the glioma microenvironment were deconvoluted, revealing tumor-associated foam cells (TAFCs) as the most abundant and centrally connected subtype. The high expression of two prognostic candidate genes, growth differentiation factor 8 (GDF-8, also known as myostatin, ) and transcription factor 12 (), in TAFCs was found to be correlated with poor overall survival. These two genes were associated with M2 macrophage infiltration, altered cholesterol homeostasis, and immunosuppressive signaling. Regulatory network and pathway analyses, based on computational motif enrichment and co-expression analysis, linked them to ribosome, Notch signaling, DNA repair, and cell-cycle pathways. Pseudotime trajectories revealed dynamic expression during differentiation. Additionally, drug sensitivity prediction analysis demonstrated that expression was significantly associated with sensitivity to paclitaxel and VE-822, while expression showed potential associations with sensitivity to cytarabine, olaparib, Wee1 inhibitor, paclitaxel, and VE-822. Logistic regression analysis combining clinical parameters with and expression effectively achieved risk stratification for glioma, with higher composite scores predicting worse 2- and 3-year survival outcomes. Calibration curves demonstrated high consistency between nomogram-predicted overall survival probabilities and actual observed outcomes. Immunofluorescence confirmed upregulated expression of and in glioma tissues and their co-localization with macrophages. In conclusion, this study identified TAFCs as the central cells in the glioma microenvironment, with their signature genes and representing candidate immunometabolic signatures associated with macrophage-mediated immunosuppression and metabolic reprogramming in glioma, suggesting their potential as biomarkers for patient stratification and as targets for immunometabolic therapies. - Source: PubMed
Publication date: 2026/03/09
Liu XuSong ZhuoSun ZhijiaLiu ChenKang XiaoliQiao HuilianTu XinzhuoLi TengFu ZhiguangWang Yingjie - Indicine cattle () show notable resilience and disease resistance compared with taurine breeds, but the genomic basis of these traits remains largely unexplored. Identification of genomic elements for immunity will enable future controlled crossbreeding programs using molecular breeding methods. Therefore, we performed a genome-wide comparison among Nelore, Gir, and Hereford breeds using their whole-genome sequences, majorly focusing on immune-related structural and sequence variation. Our aims were to catalog insertions, deletions, and single nucleotide variants (SNVs) that intersect immune loci and known quantitative trait loci (QTLs), identify runs of homozygosity and selective-sweep signals, and prioritize candidate genes for follow-up functional studies. We retrieved whole-genome sequencing data for Nelore breed ( = 14) and Gir breed ( = 20) from NCBI using the SRA toolkit. Reads were checked with FastQC v0.12.1, filtered with Fastp 0.23.4 to remove low-quality bases and adaptors, and retained high-quality reads based on Q20 and Q30. The reads were mapped to the reference (ARS-UCD2.0) with Burrows-Wheeler Aligner - Maximal Exact Matches (BWA-MEM) v0.7.19-r1273; alignments were processed with Samtools 1.19.2 for sorting, duplicate marking, and MAPQ ≥ 20 filtering. Variants (insertions, deletions, SNVs) were called with GATK HaplotypeCaller (GATK v4.4.0.0), hard-filtered, normalized with Bcftools 1.19, and annotated with SnpEff 5.2b and SnpSift v5.2 f. Common variants were identified via in-house Python scripts; immune loci were detected from InnateDB and keyword searches; QTL overlaps were identified using Animal QTLdb; and DAVID was used for GO and KEGG enrichment ( < 0.05). ROH islands were defined in PLINK as regions shared by > 50% of individuals or samples, and selective sweeps were detected with RAiSD; genes overlapping ROH islands and RAiSD peaks were prioritized as candidate selection signatures. GATK v4.4.0.0 identified 1,884,058 indels and 13,997,533 SNVs in Nelore breed, and 1,457,337 indels and 11,627,881 SNVs in Gir breed, with Ti/Tv ratios of ~ 2.26 and ~ 2.25, respectively. Nelore breed has more number of variants than Gir. We observed frameshift insertions in and in both the breeds and frameshift deletions in in Nelore breed and in Gir breed. The variants were also identified in the regulatory regions of both breeds. The high-impact SNVs were in and genes in Nelore breed, and gene in Gir breed. Genome-wide scans using RAiSD identified selective sweeps in 707 candidate genes in Nelore breed and 165 in Gir breed. Comparing the ROH and RAiSD results, we prioritized the genes ,,,,, and in Nelore breed, and the genes and in Gir breed. These genes are found in QTLs linked to milk and health traits. Functional enrichment showed that the genes exhibiting all the three variants belong to immune pathways such as, NF-kappaB signaling, T-cell receptor signaling, and MAPK signaling in both breeds. These results reveal breed-specific genomic variation locating immune loci and its associated QTLs and provide a list of candidate genes and regions for experimental validation and marker development to improve disease resistance and productivity in Indicine cattle. - Source: PubMed
Publication date: 2026/03/16
Thambiraja MenakaIyengar Shukrruthi KSatishkumar BrinthaKavuru Sai RohithKatari AakankshaSingh DheerOnteru Suneel KYennamalli Ragothaman M - The domestic chicken (Gallus gallus) is a critical model for immunology and a major agricultural species, yet a comprehensive single-cell census of its immune architecture is lacking. Here, we constructed a high-resolution single-cell transcriptome atlas of three primary immune organs (thymus, bursa of Fabricius, and spleen) from chickens. Our analysis identified 24 distinct immune and stromal cell types, mapping their organ-specific distributions: B cells were predominantly localized to the bursa of Fabricius, while T cell subsets were enriched in the thymus. We delineated developmental trajectories and identified key transcription factors governing lineage commitment, including TCF12 for CD4⁺CD8⁺ T cells and SOX13 for T helper 2 cells. Further analysis revealed potential sexually dimorphic immune maturation, where B cells exhibited sex-biased gene expression primarily in early differentiation stages, whereas T cell differences were most pronounced during proliferation. Cross-species comparison demonstrated remarkable conservation of core immunoregulatory circuits, with transcription factors like MEF2C, LEF1, and GATA3 playing conserved roles in avian and mammalian immunity. This resource provides a foundational framework for understanding avian immune defense, advancing poultry disease-resistant breeding, and offers evolutionary insights into vertebrate immunology. - Source: PubMed
Publication date: 2026/01/18
Liu HuichaoTu JunhaoHe XiOuyang QingyuanZhang Haihan - VGLL3 (encoding the mammalian Vestigial-like 3 transcriptional cofactor) has emerged as a fusion partner in hybrid nerve sheath tumors and in rare spindle cell rhabdomyosarcomas (RMS) of the head and neck. We herein describe a new EP300::VGLL3 RMS and review/update reported cases (total: 6) to reappraise their outcome. All six reported EP300::VGLL3 fusion RMS cases originated exclusively in the tongue musculature of adult males at a median age of 48 years (range, 36-59). Nonradical surgery was the initial treatment in most cases, followed by variable re-excisions in most. Incomplete adjuvant chemotherapy was given to one patient. No metastases were recorded, and all patients with follow-up were disease-free at last follow-up (12, 34, 36, and 48 months). Histologically, all tumors displayed bland spindled to ovoid plump cells lacking clear-cut rhabdomyoblastic features and disposed into fascicular and storiform patterns. The tumor margins were infiltrating with entrapment of skeletal muscle fibers. Overtly malignant cytology, brisk mitotic activity, necrosis, lymphovascular, and perineural invasion were absent. Immunohistochemistry was consistently positive for desmin (6/6), and variably myogenin (6/6), myoD1 (4/4), and SMA (5/6). Fusion breakpoints were identical among all cases (EP300ex31::VGLL3ex2). Given their indolent course after local excision alone, the noncommitted term EP300::VGLL3-fused rhabdomyoblastic tumor might be more appropriate for these tumors than the original RMS terminology to avoid overprognostication/overtreatment that the "rhabdomyosarcoma" label would imply. Reporting more cases is mandatory to elucidate the full anatomic and biological spectrum of this morphologically, anatomically, and genetically unique entity. - Source: PubMed
Publication date: 2026/02/06
Agaimy AbbasThway KhinFisher Cyril