NFYC antibody - C-terminal region (P100681_P050)
- Known as:
- NFYC (anti-) - C-terminal region (P100681_P050)
- Catalog number:
- p100681_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- NFYC antibody - C-terminal region (P100681_P050)
Related genes to: NFYC antibody - C-terminal region (P100681_P050)
- Gene:
- NFYC NIH gene
- Name:
- nuclear transcription factor Y subunit gamma
- Previous symbol:
- -
- Synonyms:
- CBF-C, NF-YC
- Chromosome:
- 1p34.2
- Locus Type:
- gene with protein product
- Date approved:
- 1996-10-26
- Date modifiied:
- 2016-10-05
Related products to: NFYC antibody - C-terminal region (P100681_P050)
Related articles to: NFYC antibody - C-terminal region (P100681_P050)
- a leguminous herb native to East Asia, is renowned for its rich content of bioactive compounds, particularly triterpenoid saponins and flavonoids, within its roots. Nuclear factor Y (NF-Y) is a key transcription factor family in eukaryotes, known to regulate abiotic stress responses in plants by binding to the CCAAT-box motif in promoter regions. Despite its significance, the gene family has not been systematically identified in prior to this study. Here, we identified 34 genes from the genome and characterized their gene lengths, domain architectures, and amino acid sizes. Phylogenetic analysis classified the members into three distinct subfamilies (I, II, and III). Notably, proteins within the same clade exhibited conserved motif structures, suggesting that evolutionary relatedness may reflect functional similarities. Gene Ontology (GO) enrichment assigned the genes to 23 functional categories, indicating substantial functional diversification despite the presence of conserved protein motifs. Furthermore, based on cis-acting element analysis, we investigated the responses of genes to methyl jasmonate (MeJA) treatment. Six promoters contained MeJA-responsive elements, and genes regulated by MeJA exhibited significantly upregulated expression following MeJA treatment. This study provides the first comprehensive identification and characterization of the gene family in , establishing a foundation for future research on their roles in biological processes and stress signaling pathways. - Source: PubMed
Publication date: 2026/03/06
Hu ShengpingFei JunJin YanghuiHu JinpingShi ShiyuanCao Tianyi - Nuclear factor Y (), evolutionarily conserved heterotrimeric transcription factors (TFs), are found throughout eukaryotic organisms. Comprising the , , and subfamilies, this family is established as playing critical roles in plant growth and development. While earlier research has mainly centered on the functional and evolutionary characteristics of within individual plant species, large-scale analyses and evolutionary patterns of these genes across major plant lineages remain largely unexplored. Here, we systematically identified 15 392 nonredundant genes of family from 320 horticultural and representative plant species. Our findings showed that this gene family originated from charophytes. In bryophytes, pteridophytes, and gymnosperms, dispersed duplication served as the predominant mode of gene expansion, whereas in angiosperms, their expansion was driven by whole genome duplication/segmental, dispersed, and tandem duplication. Conserved motif analysis revealed that highly conserved motifs are present within each subfamily across eight representative plant species. However, some genes in higher plants exhibited motif loss, indicating sequence variations during their evolutionary history. Transcriptomic profiling analysis of genes in under various conditions, including hormonal treatments, abiotic/biotic stresses, as well as various developmental stages, revealed their functional versatility. Furthermore, an interaction network comprising 36 genes along with 2473 downstream and 261 upstream genes was constructed in . Enrichment analysis revealed interactions between genes and other TFs, particularly those from the _DNA-binding and APETALA2 () families, which were consistently enriched among both upstream and downstream regulatory genes. This work provides the first comprehensive and large-scale investigation into the evolutionary dynamics of genes, encompassing taxa from basal algae to advanced horticultural plants, thereby offering novel insights into their evolutionary and lineage-specific expansion. - Source: PubMed
Publication date: 2025/11/04
Luo KaiLi MingchaoLiu ManJia XitaoLi ZhouZhao XuechunChen JihuiGu XinyaoHe JinChen ChaoDong Rui - The larva of the sleeping chironomid, Polypedilum vanderplanki, is the only insect capable of extreme desiccation tolerance, known as anhydrobiosis. The larvae can survive near-complete desiccation and, upon rehydration, rapidly resume metabolism and return to their normal life cycle. Activation of genes involved in antioxidant activity, protection of biomolecules, and DNA repair is required for desiccation tolerance. In the desiccation-tolerant P. vanderplanki cell line Pv11, the key factors of the regulatory network for these genes are heat shock factor (Hsf) and nuclear transcription factor Y subunit C (NF-YC). However, how desiccation tolerance is established at the whole-body level remains unknown. To unravel the dynamic response to desiccation at this level, it is necessary to clarify which molecules need to function for desiccation tolerance and when. Here, we newly acquired and analyzed detailed time-series gene expression data to reveal the regulatory mechanisms underlying the transition to an ametabolic state during desiccation. We showed that the acquisition of desiccation tolerance requires the expression of a number of desiccation-inducible genes in a specific order. This order can be explained by a gene regulatory network triggered by nuclear transcription factor Y subunit A (NF-YA) and Sine oculis-related homeobox 4 (Six4). To our knowledge, this is the first report that desiccation tolerance is related to stepwise changes in gene expression in response to desiccation. - Source: PubMed
Publication date: 2026/01/10
Hiki YusukeYamada Takahiro GCornette RichardGusev OlegShagimardanova ElenaKikawada TakahiroFunahashi Akira - Pecan transcription factor CiNF-YC6, identified as nuclear-localized, significantly boosts fatty acid content in transgenic plants via activating lipid pathway genes upon overexpression. Pecan (Carya illinoinensis) seed oil is rich in unsaturated fatty acids beneficial for human health. Therefore, improving its quality is of considerable interest. The Nuclear Factor Y (NF-Y) transcription factor family plays essential roles in plant development and metabolism, yet its function in regulating seed lipid accumulation in woody oil-producing species remains poorly understood. Here, we identified and characterized 44 NF-Y genes in Carya illinoinensis, comprising 12 NF-YA, 20 NF-YB, and 12 NF-YC members. Phylogenetic, structural, and cis-element analyses revealed conserved features and regulatory potential across the family. Among them, CiNF-YC6 exhibited high expression in developing seeds and other tissues. Subcellular localisation and yeast assays confirmed that CiNF-YC6 is a nuclear-localized transcriptional activator with the activation domain located at the C-terminus. Functional characterization using transient expression in Nicotiana benthamiana and stable transformation in Arabidopsis thaliana showed that CiNF-YC6 overexpression significantly increased total fatty acid content and altered fatty acid composition. This enhancement was accompanied by the upregulation of key genes involved in fatty acid biosynthesis and triacylglycerol (TAG) assembly. Our findings identify CiNF-YC6 as a novel regulator of lipid accumulation and provide insights into the transcriptional control of oil biosynthesis in a woody perennial species. - Source: PubMed
Publication date: 2025/12/19
Wang LinnaZou LinaYao ZixianHe JinhuaZhang ShunranXiang Yan - Starch branching enzyme (SBE) is a key enzyme in starch biosynthesis that introduces branch points into starch molecules. Among its three isoforms, SBE3 plays a pivotal role, as its loss of function significantly alters starch granule structure and accumulation in rice, leading to chalky grains and reduced grain weight. However, the molecular regulatory network governing SBE3 expression remains largely unknown. In this study, three NF-YC family transcription factors-OsNF-YC8, OsNF-YC9, and OsNF-YC10-were identified as transcriptional activators of SBE3, each directly binding to the AAGAGG motif in the SBE3 promoter. Functional analysis revealed functional redundancy among these NF-YC members, as disruption of one or two genes had little effect on SBE3 expression. In contrast, simultaneous disruption of all three genes in the osnf-yc8/9/10 triple mutant resulted in a pronounced reduction in SBE3 transcription, accompanied by a substantial decrease in short-chain amylopectin with degrees of polymerization ranging from 6 to 24. The endosperm of osnf-yc8/9/10 appeared milky white, and resistant starch content was 3.7-fold higher than that of the wild type, without a significant reduction in grain weight. These results elucidate the regulatory roles of OsNF-YC8, OsNF-YC9, and OsNF-YC10 in SBE3 expression and short-chain amylopectin biosynthesis, highlighting their potential for developing functional rice cultivars. - Source: PubMed
Publication date: 2025/12/12
Chen JiabinBian XinyueChu RuiWang TaoYang RuiZhang ChangquanHuang LichunFan XiaoleiLiu Qiaoquan