LALBA Antibody (OASA09222)
- Known as:
- LALBA Antibody (OASA09222)
- Catalog number:
- oasa09222
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- LALBA Antibody (OASA09222)
Related genes to: LALBA Antibody (OASA09222)
- Gene:
- LALBA NIH gene
- Name:
- lactalbumin alpha
- Previous symbol:
- -
- Synonyms:
- LYZL7
- Chromosome:
- 12q13.11
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2016-01-12
Related products to: LALBA Antibody (OASA09222)
Related articles to: LALBA Antibody (OASA09222)
- Sows with high prolificacy and better lactation traits are beneficial for weaned piglet number. Because the genetic basis of sow lactation traits remains elusive, genetic improvement for lactation traits lags behind that for litter traits, constraining the full realisation of genetic potential for large litters. Here, we measured 1 060 Fourier-transform infrared (FTIR) wavenumbers and five predicted colostrum composition traits from sow colostrum samples. Heritability estimates for both the FTIR spectra and predicted traits ranged from moderate to high. Correlation analysis revealed that lactose percentage was negatively genetically correlated with the other four predicted traits (fat percentage, protein percentage, total solid content, and urea nitrogen content) and with 24 h litter weight, which was positively genetically correlated with both protein and total solid content. Genome-wide association studies on the FTIR spectra and predicted traits identified 134 significant single-nucleotide polymorphisms (SNPs) (False discovery rate < 0.05), with most clustering on Sus scrofa chromosomes (SSC) 5 and 7. Among the candidate genes, two expressed in lactating mammary tissue have established roles in milk trait determination: (1) LALBA, which encodes a major colostrum protein and is responsible for lactose synthesis, and (2) BTN1A1, which mediates milk fat secretion. Additionally, the study detected two important candidate variants on SSC7: (1) rs691487382, which was colocalised with the expression quantitative trait locus signal for TRIM26 in the liver, a key metabolic organ supporting lactation, and (2) rs327923027, a missense variant located in a phylogenetically conserved domain of TRIM26, an E3 ubiquitin ligase implicated in liver homeostasis. Taken together, this study identifies, for the first time, candidate genes and variants for sow colostrum, providing genomic markers useful for genetic improvement. - Source: PubMed
Publication date: 2026/08/20
Liang HZhang BKang J CMa KLi X PYin L LYu M - Tumor-suppressive immunity is more evident in early-stage compared to advanced tumors making it the ideal point for cancer interceptive immunotherapies. We previously described that higher peripheral T cell diversity is associated with more pronounced CD8 T cell infiltration in ductal carcinoma in situ of the breast, implying a close interaction between peripheral and intratumor immunity. Here, we developed lipid nanoparticle (LNP)-encapsulated messenger ribonucleic acid (mRNA) vaccines expressing the alpha-lactalbumin (LALBA) protein unique to mammary luminal progenitors (LPs) to test whether enhancing immune response by prophylactic vaccination against the putative cell-of-origin of breast cancer suppresses tumorigenesis. Vaccination of outbred Sprague-Dawley rats with N1-methylpseudouridine-modified or unmodified Lalba mRNA-LNP induced different degrees of LALBA-specific and nonspecific immune responses. The vaccination suppressed carcinogen-induced mammary tumorigenesis and improved tumor-free and overall survival without obvious toxicity in the normal mammary glands and other organs. Single-cell transcriptomic analysis revealed that vaccination decreases the frequency of a proliferative LP population in immunoreactive early epithelial hyperplasia. Overall, we provide proof of principle that prophylactic Lalba mRNA-LNP has the potential to suppress the initiation and progression of early breast neoplastic lesions. - Source: PubMed
Publication date: 2026/09/02
Nishida JunSeehawer MarcoRojas Jimenez ErnestoYan PengzeBui Triet MFoidart PierreMuramatsu HiromiGoyette Marie-AnneCai XinranLi ZheqiBeattie Mitchell BPardi NorbertPolyak Kornelia - Lactation and mammary involution and remodeling are complex biological processes that require the coordinate expression of thousands of genes. Differential expression analyses comparing lactating and dry goats have revealed extensive changes in the expression of protein-coding and non-coding RNAs in the mammary gland. Here, we hypothesize that lactation and mammary involution/remodeling may also involve changes in the abundance of transcripts differing in exon composition and functional properties. To test this hypothesis, we analyzed the mammary transcriptomes of 5 lactating and 4 dry goats by using a hybrid approach based on the integration of data from short-read Illumina and long-read Nanopore sequencing. After data filtering, we detected 21,598 transcripts derived from 12,300 genes (≈1.7 transcripts per locus) in the goat mammary gland. Among them, we found 14,092 annotated isoforms, 6,291 novel isoforms, and 1,215 novel loci. Around 39.3% of expressed genes generated multiple transcript variants. Overall, the goat mammary transcriptome showed exon skipping as the most frequent splicing event, followed by alternative use of initial exons, intron retention, and variations in 3' and 5' splicing sites. We also observed that a limited number of isoforms accounted for a very substantial fraction of the total expression output of the lactating mammary gland, with the top 10 and top 50 genes representing approximately 66% and 82% of total expression, respectively. This transcriptomic specialization is driven primarily by genes encoding caseins CSN1S1, CSN2, and CSN3, and major whey proteins such as progestagen associated endometrial protein (PAEP), α-lactalbumin (LALBA), and lactophorin (GLYCAM1), which are essential milk nutrients. Finally, differential transcript usage (DTU) analysis comparing lactating and dry goats revealed 443 isoform switches affecting 355 unique genes and 563 transcripts. Besides, 266 and 297 transcripts were upregulated and downregulated in lactating goats, respectively, and 413 DTU, affecting 248 genes, were predicted to have functional consequences. Among these functional consequences, the most important ones were protein domain gain, non-reference domain isoform gain, nonsense-mediated insensitivity and coding transcripts. Several of the genes showing DTU have important roles in lactation, being of particular relevance those encoding epidermal growth factor receptor (EGFR), glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), hydroxysteroid 11-β dehydrogenase 1 (HSD11B1), insulin receptor substrate 1 (IRS1), nuclear receptor subfamily 3 group C member 1 (NR3C1), and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1). Moreover, we also detected DTU for several genes integrated in the mitogen-activated protein kinase and Rho GTPase pathways. In summary, we provide a comprehensive catalog of RNA isoforms expressed in the goat mammary gland and demonstrate that mammary transcript splicing patterns differ substantially between lactating and dry goats. - Source: PubMed
Publication date: 2026/07/27
Cardoso T FWang MNoce ALuigi-Sierra MMartínez ADelgado-Bermejo J VSalama A A KSuch F XJordana JAmills M - This study investigated the effect of dietary thiamine supplementation on mammary inflammation, antioxidant function and lactation performance of heat-stressed Hu ewes. Twenty-four lactating Hu ewes (parity 1-2; days in milk = 14 ± 1 d; milk yield = 1.25 ± 0.08 L/d) with body weight of 52.6 ± 2.7 kg and body condition score of 2.62 ± 0.12 (0 = emaciation, 5 = obesity) were selected. After a 7 d acclimation period, the ewes were randomly allocated into three treatments and raised for 21 d under different ambient temperatures: thermoneutral group without supplementation (CON), heat stress group without supplementation (HS), and heat stress group with dietary thiamine supplementation at 100 mg/kg dry matter intake (DMI) (THS). Each group consists of 8 repetitions, and each repetition involves 1 ewe. THS group significantly increased milk yield, milk fat, lactose, and protein levels, except for DMI, compared with HS group ( < 0.05). The THS group significantly decreased DMI, milk yield, milk fat, lactose, and protein levels compared with CON group ( < 0.05). Milk fatty acid (FA) production of preformed FA, C18:0, C18:1n9c, C18:2n6c, C18:3 and C20:3 of THS group significantly decreased compared with HS group ( < 0.05). Milk FA production of preformed FA, C18:0, C18:1n9c, C18:2n6c, and C20:3 of THS group significantly increased compared with CON group ( < 0.05). Arginine, leucine, valine, alanine, and aspartate productions in milk of THS group were significantly higher than those in HS group ( < 0.05). Relative to the HS group, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities and triglyceride content were significantly higher in the THS group ( < 0.05), while malondialdehyde (MDA) and TNF-α, IL-6, and IL-1β levels significantly declined in the THS group ( < 0.05) in mammary vein blood and mammary tissue. Relative to the HS group, the protein abundances of lactose synthesis related proteins (GLUT1, GLUT3, and LALBA), milk fat synthesis related proteins (FASN, SCD, and PPARγ) and milk protein synthesis related proteins (CAT1, EAAT3, and ASCT2) were significantly higher in the mammary tissue in the THS group ( < 0.05). Relative to the CON group, the protein abundances of lactose synthesis related proteins (GLUT1, GLUT3, and LALBA), milk fat synthesis related proteins (FASN, SCD, and PPARγ) and milk protein synthesis related proteins (CAT1, EAAT3, and ASCT2) were significantly lower in the mammary tissue in the THS group ( < 0.05). These results suggest that thiamine supplementation could mitigate mammary inflammation, improve mammary antioxidant function and lactation performance. - Source: PubMed
Publication date: 2026/06/06
Wu HuisiNi TingtingYou FengHuang YumingHe FeiyangLiu GaoWang JianingWang MengzhiZhang Hao - Human milk oligosaccharides (HMOs) are unconjugated and structurally diverse glycans synthesized in the lactating mammary gland through the stepwise action of glycosyltransferases that extend a free lactose core. Several HMOs are capped with sialic acids, including 3'-sialyllactose (3'-SL) and 6'-sialyllactose (6'-SL), that promote early-life microbiota development and contribute to immune system and neuronal functions. These health-promoting properties make sialylated HMOs attractive biomolecules for incorporation in infant nutrition and functional food products. Mammalian cell lines lack endogenous HMO production, limiting mechanistic studies of HMO biosynthesis and constraining production strategies based on human cells. Here, we developed a human cell-based strategy for the production of the two common sialyllactose isomers 3'-SL and 6'-SL in glycoengineered human embryonic kidney (HEK293) cells. We co-expressed LALBA and B4GALT1, that together form the lactose synthase complex, to introduce free lactose biosynthesis capacity into a genetically engineered human cell line without sialylation (HEK293ΔSia). Stable expression of either ST3GAL or ST6GAL isoenzymes in HEK293ΔSia cells revealed that ST3GAL3/4/5, and especially ST3GAL5, efficiently convert lactose into 3'-SL while ST6GAL1 and ST6GAL2 produce the 6'-SL isomer. These results provide insights into the in vivo ability of sialyltransferase isoenzymes to use lactose as substrate. Establishing HMOs biosynthesis pathways into controllable human cell systems offers an alternative strategy for production of HMOs and provides a starting point to unlock biosynthesis of more complex HMOs in human cells. - Source: PubMed
Kruf StijnDelahaije Roy J B MMohamed Khadra ASchoemaker BarryNarimatsu YoshikiClausen HenrikTriantis VassilisBoltje Thomas JBüll Christian