MOV10L1 Antibody (OAAF06635)
- Known as:
- MOV10L1 Antibody (OAAF06635)
- Catalog number:
- oaaf06635
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- MOV10L1 Antibody (OAAF06635)
Related genes to: MOV10L1 Antibody (OAAF06635)
- Gene:
- MOV10L1 NIH gene
- Name:
- Mov10 like RISC complex RNA helicase 1
- Previous symbol:
- -
- Synonyms:
- DJ402G11.8, DKFZp434B0717, CHAMP
- Chromosome:
- 22q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 2000-05-31
- Date modifiied:
- 2018-06-07
Related products to: MOV10L1 Antibody (OAAF06635)
Related articles to: MOV10L1 Antibody (OAAF06635)
- PIWI-interacting RNAs (piRNAs), PIWI proteins, and piRNA biogenesis-associated proteins form a conserved RNA network that protects germline genome integrity and is increasingly linked to cancer biology. Most cancer reviews have emphasized altered piRNA abundance or PIWI expression, whereas the upstream machinery that generates, loads, trims, and stabilizes piRNAs has received less critical attention. For the RNA Networks and Biology section, this Review re-evaluates piRNA biogenesis proteins as RNA-network regulators rather than as isolated tumor biomarkers. We summarize the biochemical logic of precursor selection, mitochondrial processing, PIWI loading, 3-end maturation, and effector complex assembly, focusing on PIWIL1-4, PLD6/MitoPLD, MOV10L1, DDX4/VASA, GASZ, TDRKH, PNLDC1, HENMT1, Tudor-domain proteins, HSP90, and MAEL. We then assess cancer evidence by distinguishing direct perturbation studies from expression associations, animal-germline mechanisms, and computational inference. Current evidence supports tumor-context-specific roles for PIWIL1, PIWIL2, DDX4, TDRD1, MAEL, and emerging PLD6 biology in selected tumors, whereas several canonical maturation or scaffold proteins remain underexplored in human cancer. We propose that dysregulated piRNA biogenesis proteins may influence cancer through piRNA-dependent small-RNA imbalance and piRNA-independent reactivation of germline RNA-processing, epigenetic, metabolic, and immune programs. Finally, we discuss biomarker and therapeutic opportunities while emphasizing unresolved controversies, technical limitations in piRNA detection, reproductive toxicity, and the need for causal validation in human tumor models. - Source: PubMed
Publication date: 2026/07/29
Peng SiyunWei BoLi XingLi Ermao - Non-obstructive azoospermia represents one of the most intractable forms of male infertility, with its onset frequently associated with meiotic abnormalities. To date, the pivotal molecular mechanisms governing meiosis remain incompletely understood. This study demonstrates that CMTR2 (Cap methyltransferase 2) exerts a critical regulatory function in male germ cell meiosis through a mechanism independent of its methyltransferase activity. Germ cell-specific Cmtr2 knockout mice (Ddx4-GcKO) exhibited meiotic prophase I zygotene arrest and male sterility. Ddx4-GcKO spermatocytes displayed phenotypic abnormalities, including defective DNA double-strand break repair and compromised crossover formation and recombination. Subsequent investigations revealed a marked reduction in the messenger RNA (mRNA) stability of key meiotic genes, Speedy/RINGO cell cycle regulator family member A (Spdya) and moloney leukemia virus 10 Like 1 (Mov10l1), in Ddx4-GcKO testes. Mechanistic studies indicated that CMTR2 directly interacts with the 5' untranslated regions of these genes. Importantly, CMTR2 selectively mediates 2'-O-methylation of Spdya mRNA, whereas its regulation of Mov10l1 mRNA stability is independent of its enzymatic activity. This study is the first to uncover the non-canonical role of CMTR2 in meiotic regulation, enhancing our comprehension of meiotic molecular mechanisms and offering novel insights into the etiology of male sterility. - Source: PubMed
Zheng BoGao WenxinWu HaoZhao DanWu TiantianHu HaoyueJiang BingQu WenyingFeng YuxuanLiang YutingYu JunHuang XiaoyanChen XiaWang KehanSun Fei - Highly pathogenic avian influenza viruses (HPAIVs) of the H5N8 subtype have caused severe outbreaks in poultry and wild birds worldwide. Disease outcome differs markedly between avian species: chickens typically develop acute infection with high mortality, whereas ducks often exhibit milder or delayed disease. The molecular basis underlying these species-specific differences in early host responses remains incompletely understood. To characterize early transcriptional responses to HPAIV infection, RNA sequencing (RNA-seq) was performed on lung tissues from chickens and ducks experimentally infected with H5N8 HPAIV at 24 hours post-inoculation (hpi). Differential gene expression analysis and functional enrichment analyses were conducted, and selected host genes were validated by quantitative real-time PCR. At 24 hpi, a comparable number of differentially expressed genes (DEGs) was identified in chickens and ducks; however, the organization of the transcriptional response differed markedly between species. Chickens exhibited a pathway-centered transcriptional profile dominated by innate immune and inflammatory signaling, whereas ducks displayed a more distributed and balanced transcriptional response spanning multiple functional categories. Functional enrichment analyses revealed strong activation of interferon- and cytokine-associated pathways in chickens, while transcriptional changes in ducks were dispersed across processes related to RNA metabolism, antiviral regulation, and cellular homeostasis. Among species-specific differentially expressed genes, MOV10L1 showed opposite regulation in these bird species. These findings demonstrate that early host responses to H5N8 HPAIV infection differ between chickens and ducks not only in gene identity but also in transcriptional organization. The distributed response architecture observed in ducks may contribute to controlled antiviral responses with limited immunopathology. This study provides a comparative transcriptomic framework for understanding species-specific host responses to HPAIV infection in poultry. - Source: PubMed
Publication date: 2026/06/11
Sajewicz-Krukowska JoannaDomańska-Blicharz KatarzynaTarasiuk KarolinaŚmietanka KrzysztofMarzec-Kotarska Barbara - Spermatogenesis is a complex differentiation process requiring precise spatiotemporal gene regulation, yet the functions of most testis-enriched long noncoding RNAs (lncRNAs) remain elusive. Here, we identify as a pachytene-specific lncRNA that is essential for mouse spermatogenesis. Reanalysis of single-cell RNA-seq reveals that expression is minimal in spermatogonia and preleptotene cells, sharply increases during the pachytene stage, and declines after meiosis, precisely coincides with the wave of pachytene piRNA production. In vivo knockdown of in mouse testes via AAV9-shRNA severely disrupts spermatogenesis, causing a reduction in testis size, seminiferous epithelium thickness, and epididymal sperm count, suggesting its physiological necessity. Mechanistically, we demonstrate that functions as a bona fide piRNA precursor: it harbors numerous piRNA sequences, and its overexpression in GC-2 spd(ts) cells specifically upregulates corresponding overlapping piRNAs. This processing relies on the core piRNA machinery, and co-overexpression of , together with or , promotes piRNA production. Notably, this piRNA precursor function is evolutionarily conserved, as the human homolog , which is linked to asthenozoospermia, also generates piRNAs when expressed in GC-2 spd(ts) cells. Transcriptomic analysis following overexpression in GC-2 spd(ts) cells reveals dysregulation of genes involved in metabolism and signaling, with several spermatogenesis-related genes (e.g., , ) predicted as direct targets of -derived piRNAs. Our study identifies as a pachytene-specific lncRNA essential for male fertility and demonstrates that it functions as a conserved piRNA precursor. These findings provide mechanistic insight into how an individual lncRNA contributes to the piRNA pathway and suggest that it may operate through dual mechanisms in mammalian spermatogenesis. - Source: PubMed
Publication date: 2026/05/11
Jian ShikunHan XuXie ChenyaoMa YinghaoChen PeizeLiu WeiguangXiao JunSong Xiaoyuan - Pachytene piRNAs are the least understood class of piRNAs in the mammalian male germ line. During meiosis, their biogenesis occurs near the mitochondrial outer membrane in germ granules known as intermitochondrial cement (IMC). However, how mitochondrial factors regulate the trafficking of PIWI proteins into and out of the IMC remain poorly understood. Here we show that the cytoplasmic PIWI proteins MILI and MIWI are recruited for pachytene piRNA biogenesis via distinct mitochondrial membrane proteins. Loss of the mitochondrial scaffold protein ASZ1 during meiosis in mice disrupts multiple downstream biogenesis steps, resulting in misregulation of MILI, MIWI, and MOV10L1, failure of IMC formation, and an almost complete loss of mature pachytene piRNAs. Strikingly, despite the drastic depletion of pachytene piRNAs, LINE1 transposon silencing remains unaffected. We identify three classes of pachytene piRNA pathway components that coordinate piRNA production and compartmentalization. Our findings reveal that chromatoid body precursors serve as a central hub for the accumulation of pachytene PIWI-piRNA complexes, thus establishing a connection between IMC-based biogenesis and downstream piRNA function. - Source: PubMed
Publication date: 2026/05/12
Yan XiaoyuanWei ChaoMann Jeffrey MShang GuanyiWang QianyiXie HuirongDemireva Elena YSun LiangliangDing DeqiangChen Chen