VWA5A Antibody (OAAF01996)
- Known as:
- VWA5A Antibody (OAAF01996)
- Catalog number:
- oaaf01996
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- VWA5A Antibody (OAAF01996)
Related genes to: VWA5A Antibody (OAAF01996)
- Gene:
- VWA5A NIH gene
- Name:
- von Willebrand factor A domain containing 5A
- Previous symbol:
- LOH11CR2A
- Synonyms:
- BCSC-1
- Chromosome:
- 11q24.2
- Locus Type:
- gene with protein product
- Date approved:
- 1997-08-28
- Date modifiied:
- 2016-10-05
Related products to: VWA5A Antibody (OAAF01996)
Related articles to: VWA5A Antibody (OAAF01996)
- Prostate cancer is one of the most common malignant tumors of the male genitourinary system. The impaired activity of natural killer (NK) cells observed in prostate cancer may contribute to immune evasion. This study aimed to develop robust NK cell-related diagnostic signatures. - Source: PubMed
Publication date: 2026/06/03
Chen ZhengjunZhou FangTian JingzhiLv QianWang DongFan Shida - Skin cutaneous melanoma (SKCM) is an aggressive malignancy where regulatory T cells (Tregs) drive an immunosuppressive tumor microenvironment, resulting in poor prognosis. Thus, there was an urgent need to identify Treg-related molecular biomarkers to optimize SKCM's prognostic assessment and therapeutic strategies. In this study, SKCM-related datasets were acquired from public databases. First, gene modules associated with Tregs screened using Weighted Correlation Network Analysis were intersected with differentially expressed genes to obtain Treg-DEGs. Subsequently, univariate Cox proportional hazards regression, LASSO regression, and multivariate Cox proportional hazards regression were employed to construct a prognostic biomarker signature. Furthermore, the biological functions of the prognostic biomarkers were explored by integrating functional enrichment analysis, molecular regulatory network construction, and drug prediction analysis. Finally, in cellular experiments, the mRNA and protein expression levels of the biomarkers were validated using qRT-PCR and Western blot. The risk model constructed based on the 10 prognostic biomarkers (PTPRF, ULK1, TGM3, CRABP2, SV2A, HLA-DQB2, KHDRBS3, VWA5A, CRIP1, and TFAP2C) could well predict the overall survival of SKCM patients. Functional enrichment analyses indicated that high-risk patients were enriched in keratinization pathways, whereas low-risk patients showed activation of autoimmune and infection-related pathways. NEAT1 might have regulated CRABP2 via miR-375. Additionally, 54 potential drugs, including resveratrol and metronidazole, were predicted for targeted therapy. qRT-PCR and Western blot confirmed PTPRF, ULK1, TGM3, and CRABP2 were upregulated at both the mRNA and protein levels. These findings indicate that the Treg-related signature serves as robust prognostic biomarkers and may guide personalized immunotherapy in SCKM. - Source: PubMed
Publication date: 2025/11/25
Lian ChaoJin RuinaZhang Xuanfen - BCSC-1 (breast cancer suppressor candidate-1), the official gene symbol VWA5A (von Willebrand factor A domain containing 5 A), is downregulated in a variety of cancer types including breast cancer. The role of BCSC-1 in tumorigenesis and the underlying mechanisms are poorly understood. One of the ways to predict a spectrum of functions of an unknown protein is to identify the protein-protein interaction network involving it. Flag-pLV-Neo-BCSC1 or the empty vector were stably transfected into breast cancer cells MCF-7. The levels of BCSC1 expression were identified by Western blotting. Co-immunoprecipitation assay coupled with liquid chromatography with tandem mass spectrometry (Co-IP-MS) and bioinformatic analysis were done to study protein-protein interactions. A subset of interacting proteins was validated by immunofluorescence (IF). IF was also conducted to determine the cell migration of BCSC-1. The pEGFP-C3 plasmids with full-length and truncated BCSC-1 were transiently transfected into breast cancer cells to identify domains of BCSC-1 guiding the subcellular location. 341 interacting partners of BCSC-1 were significantly enriched in Flag-BCSC-1 against negative control. These interactors included the lysosome and the proteasome related to proteostatic pathways, the peroxisome involved in lipid metabolism, endocytosis components in the vesicle transport pathway, and the regulation of actin cytoskeleton. STAT1, STAT3, and CDC42, identified among the interacting partners, co-localized with BCSC-1. In addition, BCSC-1 distributed to the leading edge of migrating breast cancer cells. VIT (vault protein inter-α-trypsin) domain of BCSC-1 is required in the regulation of cell migration. BCSC-1 might exert diverse functions through protein-protein interactions. - Source: PubMed
Publication date: 2025/10/21
Ma YuanZhang QingchenJu JiyuFu XiaoyanDu HongbinZhong PeiZhang ShengyanLi ZhuoweiLiu YuchenGong LeiZhao Chunling - Plumage color is an intuitive external poultry characteristic with rich manifestations and complex genetic mechanisms. In our previous study, we observed that there were more dark variations in plumage color in the F population derived from the hybridization of 2 white duck varieties. Therefore, based on the statistics of plumage color of 308 F2 populations, we further used the resequencing data of these individuals to detect copy number variations (CNVs) in the whole genome and conducted genome-wide association studies (GWAS) to determine the genetic basis related to plumage color traits. The CNV detection revealed 9,337 CNVs, with an average length of 15,950 bp and a total length of 142.02 MB, accounting for approximately 12.91% of the reference genome. The CNV distribution on the chromosomes was relatively uniform, and the number of CNVs on each chromosome positively correlated with the length of the chromosome. In the pure black plumage group, 2,101 CNVs were only identified, and 1,714 were specifically identified in the pure white plumage group. Ten CNVs were randomly selected for validation using quantitative real-time PCR, and 9 CNVs had the same CNV types as predicted, with an accuracy of 90%. Based on GWAS, we identified 2 CNVs potentially associated with plumage color variations, with the associated CNV regions covering 9 genes. Enrichment analysis of these 9 candidate genes showed significant enrichment of 3 pathways (ribosome biogenesis in eukaryotes, RNA transport, and protein export) and 17 gene ontology terms. Among these, VWA5A can downregulate MITF by binding to the regulatory factors SOX10. The occurrence of CNV may indirectly contribute to duck plumage color variation by affecting the regulatory factors of the switch gene MITF in the melanogenesis pathway. These findings have improved the understanding of the genetic basis of duck plumage color variation and have been beneficial for developing and using plumage color traits in subsequent poultry breeding. - Source: PubMed
Publication date: 2024/07/17
Zhang YiLi XiaofanGuo QixinWang ZhixiuJiang YongYuan XiaoyaChen GuohongChang GuobinBai Hao - Distant metastasis is the leading cause of death in breast cancer (BC). The timing of distant metastasis differs according to subtypes of BCs and there is a need for identification of biomarkers for the prediction of early and late metastasis. To identify biomarker candidates whose abundance level can discriminate metastasis types, we performed a high-throughput proteomics assay using tissue samples from BCs with no metastasis, late metastasis, and early metastasis, processed data with machine learning-based feature selection, and found that low VWA5A could be responsible for shorter duration of metastasis-free interval. Low expression of VWA5A gene in METABRIC cohort was associated with poor survival in BCs, especially in hormone receptor (HR)-positive BCs. In-vitro experiments confirmed tumor suppressive effect of VWA5A on BCs in HR+ and triple-negative BC cell lines. We found that expression of VWA5A can be assessed by immunohistochemistry (IHC) on archival tissue samples. Decreasing nuclear expression of VWA5A was significantly associated with advanced T stage and lymphatic invasion in consecutive BCs of all subtypes. We discovered lower expression of VWA5A as the potential biomarker for metastasis-prone BCs, and our results support the clinical utility of VWA5A IHC, as an adjunctive tools for prognostication of BCs. - Source: PubMed
Publication date: 2024/01/30
Koh JiwonJeong DabinPark Soo YoungHan DohyunKim Da SolKim Ha YeonKim HyeyoonYang SohyeonKim SunRyu Han Suk