Rpia antibody - middle region (ARP55439_P050)
- Known as:
- Rpia (anti-) - middle region (ARP55439_P050)
- Catalog number:
- arp55439_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- Rpia antibody - middle region (ARP55439_P050)
Related genes to: Rpia antibody - middle region (ARP55439_P050)
- Gene:
- RPIA NIH gene
- Name:
- ribose 5-phosphate isomerase A
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 2p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-09-30
- Date modifiied:
- 2015-08-25
Related products to: Rpia antibody - middle region (ARP55439_P050)
Related articles to: Rpia antibody - middle region (ARP55439_P050)
- Triptolide, a diterpenoid epoxide derived from Tripterygium wilfordii Hook F, exhibits potent therapeutic activities but is clinically limited by severe multiorgan toxicity, the mechanisms of which remain poorly understood. This study aimed to elucidate these mechanisms using an integrative computational framework. - Source: PubMed
Publication date: 2026/04/24
Wang YingLin Bing - Elevated phosphate (PO) levels in livestock wastewater (LSWW) significantly inhibit microalgal bioremediation, though the underlying mechanisms remain poorly characterized. This study investigated the physiological and molecular responses of microalgae to high PO stress at concentrations relevant to LSWW systems. Results demonstrated significant physiological stress and impaired nutrient uptake, with a 96.83% reduction in PO removal efficiency and a 62.28% reduction in removal rate. These responses resulted from a coordinated feedback inhibition, with enhanced intracellular phosphorus (P)-related metabolism but suppressed active PO transport. Specifically, stress adaptation was mediated through the coordinated upregulation of pentose PO pathway (e.g., genes encoding RBKS, rpiA, G6PD), glycolysis/gluconeogenesis (e.g., genes encoding talA, PFK, PFP) and phosphatidylinositol metabolism (e.g., genes encoding PIP5K, SAC1, IMPA, mmsA), collectively promoting NADPH regeneration, energy homeostasis, membrane transport, and cell signaling. ATP production was elevated through oxidative phosphorylation (e.g., genes encoding ppa and PMA1) whereas ATP conservation was achieved by downregulation genes encoding ABC transporters, despite concomitant oxidative stress and membrane destabilization. High PO suppressed key PO transporters genes (PHT1, PHT4, PHT5) and inhibited PO-dependent enzymatic activities, including acid phosphatase and ADP-glucose pyrophosphorylase. Photosynthetic integrity was maintained via carotenoid-mediated photoprotection, which mitigated oxidative damage through singlet oxygen quenching and radical scavenging. Metabolic profiling revealed a shift from protein and polysaccharide synthesis towards lipid accumulation, with notable production of odd-chain fatty acids exhibiting favorable biodiesel properties. These findings decipher the molecular regulatory networks underlying P stress in microalgae, providing practical strategies to enhance nutrient recovery and biomass valorization in microalgae-based LSWW treatment. - Source: PubMed
Publication date: 2026/04/25
Shan WenjuQiu ShuangBai JiaweiLi XiaofanWu ZhengshuaiBi QianGe Shijian - Type 2 diabetes mellitus (T2DM) is currently one of the most prominent and global chronic conditions. Cognitive decline is one of the major complications of T2DM, but its precise molecular mechanism remains unclear. Metabolomics and proteomics were combined in this study to investigate alterations in metabolites and proteins in the hippocampus of T2DM rats. KEGG Markup Language (KGML) network analysis was conducted to integrate underlying relationships among differentially expressed metabolites and proteins. 58 significantly differentially expressed metabolites and 61 differentially expressed proteins were identified between T2DM and CON rats. In proteomic analysis, GO analysis showed that DEPs involved in biological process were mainly related to neurofilament cytoskeleton organization, postsynaptic actin cytoskeleton organization and actin filament severing. KEGG pathway analysis showed the major enriched pathways were thiamine metabolism, cholesterol metabolism, pentose phosphate pathway (PPP), ABC transporters and regulation of actin cytoskeleton. In metabolomics analysis, KEGG pathway analysis showed the major enriched pathways were autophagy, lysosome, glycolysis/gluconeogenesis, PPP and ABC transporters. KGML network analysis revealed that PPP and ABC transporters were activated in the hippocampus of T2DM rats, accompanied by the up-regulation of metabolites in two pathways. Rpia was up-regulated, which is the indicator of increased PPP flux. Tap1, the unique immune-function ABC transporter, was up-regulated. Excessive PPP activation disrupts cognition-related synaptic transmission, while up-regulated immune-function ABC transporters drive aberrant synaptic remodeling and chronic neuroinflammation. These results provide a better understanding of biological mechanisms underlying T2DM-related cognitive dysfunction and may help identify potential targets for neuroprotective drugs against cognitive dysfunction in T2DM. - Source: PubMed
Zhang YingYu JialeSu DongmeiHou LiHe BinWang HuipingShi Cuige - Xylitol is industrially synthesized by d-xylose, which is more expensive than glucose. However, de novo xylitol biosynthesis from glucose remains challenging in . To address these challenges, this study first developed a biosynthetic pathway from glucose via an arabitol intermediate with arabitol phosphate dehydrogenase (APDH). Chassis optimization through knocking out key genes (, , , , ) enhanced flux and increased arabitol yield to 1.61 g/L. Subsequently, the arabitol-to-xylitol route was established using arabitol dehydrogenase (ArDH2) and xylitol dehydrogenase (XDH1) enzymes, showing a 52.1% conversion rate in vitro. The heterologous AXA module (APDH, ArDH2, XDH1) was multicopy integrated into the optimized chassis W3P-5ΔB. The strain W3P4A produced 5.04 g/L arabitol and 1.89 g/L xylitol in shake-flasks. Fed-batch fermentation with a plasmid-carrying strain achieved 5.11 g/L xylitol and 18.5% (g/g) yield from glucose. This work establishes a new platform for xylitol bioproduction from glucose and provides a foundational framework for industrial-scale implementation. - Source: PubMed
Publication date: 2026/02/20
Liu BingbingJiang LinLin LvyangYang WulongLi MianLin JianpingJiang YiqiBao ZehuaWu Mianbin - Age-related osteoporosis is increasingly recognized as a disease of stem cell dysfunction, hallmarked by the skewed lineage commitment of mesenchymal stem cells (MSCs) away from osteogenesis towards adipogenesis. This functional decline is closely linked to mitochondrial dysregulation. However, the upstream metabolic regulators that orchestrate this detrimental cell-fate switch, particularly the key nodes at the interface of mitochondrial function and cellular senescence, remain largely unknown, hindering the development of targeted therapies. To address this challenge, we implemented a bioinformatic pipeline analyzing transcriptomes of rat MSCs from GEO. Methodology included transcriptomic profiling and MitoCarta3.0 intersection to identify mitochondria-associated aging genes. A multi-algorithm strategy screened hub genes. Sixteen mitochondria-associated genes were dysregulated in MSC aging. Machine learning consistently identified ribose-5-phosphate isomerase A (RPIA), a key enzyme of the pentose phosphate pathway, as the critical hub. RPIA was up-regulated in aged MSCs, predicting senescence (area under the curve [AUROC] = 0.909). Validation in an independent dataset confirmed RPIA's up-regulation with comparable accuracy (AUROC = 0.917). To further validate the reliability of the results, we selected discarded bone tissue samples from patients aged 20 to 80 years who had undergone osteotomy, which we then examined histologically. The importance and effectiveness of RPIA have been confirmed through analysis of clinical tissue samples. At the same time, under high RPIA levels, the osteogenic potential of bone marrow mesenchymal stromal cells was significantly suppressed (P < 0.01). This study is the first to implicate RPIA in the pathobiology of skeletal aging. We propose RPIA as a metabolic switch driving mitochondrial dysfunction in senescent MSCs. Consequently, RPIA emerges as a novel, high-priority potential therapeutic target for osteoporosis, providing a compelling data-driven foundation for subsequent experimental investigation. - Source: PubMed
Publication date: 2026/02/12
Xu ZiyiGuo JianZhang JunWang XiLiao JunruZhao MengLuo XuefengMa JunchaoLiu YiboJia QiyuMa Chuang