Rpia antibody - middle region (ARP55439_P050)
- Known as:
- Rpia (anti-) - middle region (ARP55439_P050)
- Catalog number:
- arp55439_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- Rpia antibody - middle region (ARP55439_P050)
Related genes to: Rpia antibody - middle region (ARP55439_P050)
- Gene:
- RPIA NIH gene
- Name:
- ribose 5-phosphate isomerase A
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 2p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-09-30
- Date modifiied:
- 2015-08-25
Related products to: Rpia antibody - middle region (ARP55439_P050)
Related articles to: Rpia antibody - middle region (ARP55439_P050)
- Tuber dormancy length is a key agronomic trait determining potato storage quality and planting suitability, yet the underlying molecular regulatory mechanisms remain poorly understood. In this study, we perform a time-course physiological, transcriptomic, and metabolomic analysis of a long-dormancy cultivar 'Chuanliangshu 10' and a short-dormancy cultivar 'Favorita' at harvest and storage. The short-dormancy cultivar 'Favorita' exhibits a significantly faster conversion rate of starch to soluble sugars during storage compared to 'Chuanliangshu 10'. Integrated transcriptomic and metabolomic analyses revealed that 'Favorita' likely accelerates dormancy release and sprouting through the coordinated regulation of multiple metabolic pathways. First, the expression of S-adenosyl-L-homocysteine hydrolase genes (SAHH1/2) in the cysteine and methionine metabolism pathway is significantly up-regulated, accompanied by a marked accumulation of the key metabolite glutathione (GSH). Second, the phenylalanine ammonia-lyase gene (PAL) is continuously and strongly up-regulated during dormancy release, coinciding with the accumulation of the intermediate metabolites fumarate and succinate of the phenylalanine metabolism pathway. Third, expression of the zeatin-deactivating gene CKX is strongly suppressed, resulting in zeatin accumulation and thereby promoting tuber sprouting. Last but not least, the pentose phosphate pathway genes including PGD, RPIA and TKTA are significantly upregulated during storage, indicating activation of this pathway. Collectively, this study systematically elucidates the physiological, transcriptomic, and metabolomic differences between potato cultivars with contrasting dormancy characteristics, providing a theoretical foundation and candidate gene resources for precisely regulating tuber dormancy and improving potato storage traits. - Source: PubMed
Tian ShuaibingWei GuochengZhu XiaWang XiyaoFu JingyeWang Qiang - Triptolide, a diterpenoid epoxide derived from Tripterygium wilfordii Hook F, exhibits potent therapeutic activities but is clinically limited by severe multiorgan toxicity, the mechanisms of which remain poorly understood. This study aimed to elucidate these mechanisms using an integrative computational framework. - Source: PubMed
Publication date: 2026/04/24
Wang YingLin Bing - Elevated phosphate (PO) levels in livestock wastewater (LSWW) significantly inhibit microalgal bioremediation, though the underlying mechanisms remain poorly characterized. This study investigated the physiological and molecular responses of microalgae to high PO stress at concentrations relevant to LSWW systems. Results demonstrated significant physiological stress and impaired nutrient uptake, with a 96.83% reduction in PO removal efficiency and a 62.28% reduction in removal rate. These responses resulted from a coordinated feedback inhibition, with enhanced intracellular phosphorus (P)-related metabolism but suppressed active PO transport. Specifically, stress adaptation was mediated through the coordinated upregulation of pentose PO pathway (e.g., genes encoding RBKS, rpiA, G6PD), glycolysis/gluconeogenesis (e.g., genes encoding talA, PFK, PFP) and phosphatidylinositol metabolism (e.g., genes encoding PIP5K, SAC1, IMPA, mmsA), collectively promoting NADPH regeneration, energy homeostasis, membrane transport, and cell signaling. ATP production was elevated through oxidative phosphorylation (e.g., genes encoding ppa and PMA1) whereas ATP conservation was achieved by downregulation genes encoding ABC transporters, despite concomitant oxidative stress and membrane destabilization. High PO suppressed key PO transporters genes (PHT1, PHT4, PHT5) and inhibited PO-dependent enzymatic activities, including acid phosphatase and ADP-glucose pyrophosphorylase. Photosynthetic integrity was maintained via carotenoid-mediated photoprotection, which mitigated oxidative damage through singlet oxygen quenching and radical scavenging. Metabolic profiling revealed a shift from protein and polysaccharide synthesis towards lipid accumulation, with notable production of odd-chain fatty acids exhibiting favorable biodiesel properties. These findings decipher the molecular regulatory networks underlying P stress in microalgae, providing practical strategies to enhance nutrient recovery and biomass valorization in microalgae-based LSWW treatment. - Source: PubMed
Publication date: 2026/04/25
Shan WenjuQiu ShuangBai JiaweiLi XiaofanWu ZhengshuaiBi QianGe Shijian - Type 2 diabetes mellitus (T2DM) is currently one of the most prominent and global chronic conditions. Cognitive decline is one of the major complications of T2DM, but its precise molecular mechanism remains unclear. Metabolomics and proteomics were combined in this study to investigate alterations in metabolites and proteins in the hippocampus of T2DM rats. KEGG Markup Language (KGML) network analysis was conducted to integrate underlying relationships among differentially expressed metabolites and proteins. 58 significantly differentially expressed metabolites and 61 differentially expressed proteins were identified between T2DM and CON rats. In proteomic analysis, GO analysis showed that DEPs involved in biological process were mainly related to neurofilament cytoskeleton organization, postsynaptic actin cytoskeleton organization and actin filament severing. KEGG pathway analysis showed the major enriched pathways were thiamine metabolism, cholesterol metabolism, pentose phosphate pathway (PPP), ABC transporters and regulation of actin cytoskeleton. In metabolomics analysis, KEGG pathway analysis showed the major enriched pathways were autophagy, lysosome, glycolysis/gluconeogenesis, PPP and ABC transporters. KGML network analysis revealed that PPP and ABC transporters were activated in the hippocampus of T2DM rats, accompanied by the up-regulation of metabolites in two pathways. Rpia was up-regulated, which is the indicator of increased PPP flux. Tap1, the unique immune-function ABC transporter, was up-regulated. Excessive PPP activation disrupts cognition-related synaptic transmission, while up-regulated immune-function ABC transporters drive aberrant synaptic remodeling and chronic neuroinflammation. These results provide a better understanding of biological mechanisms underlying T2DM-related cognitive dysfunction and may help identify potential targets for neuroprotective drugs against cognitive dysfunction in T2DM. - Source: PubMed
Zhang YingYu JialeSu DongmeiHou LiHe BinWang HuipingShi Cuige - Xylitol is industrially synthesized by d-xylose, which is more expensive than glucose. However, de novo xylitol biosynthesis from glucose remains challenging in . To address these challenges, this study first developed a biosynthetic pathway from glucose via an arabitol intermediate with arabitol phosphate dehydrogenase (APDH). Chassis optimization through knocking out key genes (, , , , ) enhanced flux and increased arabitol yield to 1.61 g/L. Subsequently, the arabitol-to-xylitol route was established using arabitol dehydrogenase (ArDH2) and xylitol dehydrogenase (XDH1) enzymes, showing a 52.1% conversion rate in vitro. The heterologous AXA module (APDH, ArDH2, XDH1) was multicopy integrated into the optimized chassis W3P-5ΔB. The strain W3P4A produced 5.04 g/L arabitol and 1.89 g/L xylitol in shake-flasks. Fed-batch fermentation with a plasmid-carrying strain achieved 5.11 g/L xylitol and 18.5% (g/g) yield from glucose. This work establishes a new platform for xylitol bioproduction from glucose and provides a foundational framework for industrial-scale implementation. - Source: PubMed
Publication date: 2026/02/20
Liu BingbingJiang LinLin LvyangYang WulongLi MianLin JianpingJiang YiqiBao ZehuaWu Mianbin