AMN1 antibody - N-terminal region (ARP54560_P050)
- Known as:
- AMN1 (anti-) - N-terminal region (ARP54560_P050)
- Catalog number:
- arp54560_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- AMN1 antibody - N-terminal region (ARP54560_P050)
Related genes to: AMN1 antibody - N-terminal region (ARP54560_P050)
- Gene:
- AMN1 NIH gene
- Name:
- antagonist of mitotic exit network 1 homolog
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 12p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 2006-11-06
- Date modifiied:
- 2015-09-11
Related products to: AMN1 antibody - N-terminal region (ARP54560_P050)
Related articles to: AMN1 antibody - N-terminal region (ARP54560_P050)
- Multicellularity has emerged in the three branches of the tree of life. The formation of simple multicellular entities can either result from cells aggregating or staying together after mitosis. However, it is not yet fully understood how, once formed, these simple multicellular entities could be maintained or even selected for. Here, using the yeast snowflake model of simple multicellularity, we aimed at identifying genetic conditions favoring its maintenance. Growth-competition experiments revealed that, while the mutation by itself does not provide any fitness advantage or disadvantage, the snowflakes were strongly selected when combined with conditions affecting regulators of the G1/S transition of the cell cycle, such as Cln3 or Whi5. We show that this selection results from a faster exit from quiescence of the snowflake cells. Importantly, this advantage is not dependent on the multicellular phenotype, but rather on the genotype itself. We found that the selective advantage in the background fully depends on the gene, a target of the Ace2 transcription factor. Finally, we show that phenotypes observed for mutants are phenocopied by the allelic form found in 'non-laboratory' yeast strains, hence adding physiological relevance to these observations. Altogether, our results support the hypothesis that simple multicellularity could, in some cases, persist, not because it provides a direct selective advantage due to multicellularity itself, but rather as a 'passenger' phenotype that is maintained alongside other selected traits. - Source: PubMed
Publication date: 2026/06/18
Ducrocq Tom LouisLaporte DamienDaignan-Fornier Bertrand - Strains of S. cerevisiae expressing GLP-1-GIP peptide precursors with an N-terminal extension demonstrate up to 29% higher molar productivity during continuous cultivation compared to non-extended counterparts. Additionally, inactivation of the S. cerevisiae AMN1 gene, through deletion, effectively reduces broth viscosity by more than 45%, thereby enhancing oxygen transfer without adversely affecting cell metabolism or product titer. These results highlight the advantages of molecular modifications and genetic alterations for improving the production efficiency of GLP-1 receptor agonists, addressing critical challenges related to recombinant expression, viscosity and oxygen transfer in bioprocessing. - Source: PubMed
Publication date: 2026/02/05
Voulgaris IoannisNielsen Anders NygaardPetersen TineBrandt JakobJensen Sanne - Natural isolates of the yeast Saccharomyces cerevisiae were evolved under a transfer protocol that selected for cell separation and against clumpy growth. Whole-genome sequencing of haploid populations revealed strong selection to deactivate AMN1, a known regulator of postmitotic cell separation, as well as multiple instances of loss-of-function mutations to genes of the Rim101 pathway, pointing to a previously unknown role of the Rim101 pathway in regulating cell separation. In diploid populations, we observed repeated large partial deletions of chromosome III caused by fusions of the mating type loci MAT and HMR (Hawthorne's deletion) or MAT and HML (Strathern's circle). We measured the spontaneous rate of Hawthorne's deletion and found that it is within an order of magnitude of previously measured rates of whole-chromosome aneuploidy. A diploid population in which neither large deletion was detected instead fixed a heterozygous nonsynonymous mutation to the calcium channel CCH1, also pointing to a novel role for this gene in relation to cell separation. - Source: PubMed
Galeota-Sprung BenjaminPritchard ErikHuang CrystalFernandez AmySniegowski Paul - The emergence of GLP-1s for the treatment of diabetes, obesity and other diseases has led to increased focus on finding efficient ways to produce the peptides in sufficient amounts to satisfy the ever-increasing demand. Although the use of microbial hosts constitutes the cheapest, easiest and safest way to produce these peptides in high volumes, process challenges still exist that reduce the production capacity. One of the main production challenges is the high viscosity of cultivation broths, which reduces the mass and oxygen transfer, thereby creating substrate and oxygen gradients that potentially lead to unwanted secondary metabolism and eventually compromises capacity. - Source: PubMed
Publication date: 2025/06/05
Voulgaris IoannisNielsen Anders NygaardPetersen TineJensen Sanne - Environmental stressors (such as ammonia) in aquaculture could increase the risk of pathogenicity, posing a more severe threat to farmed fish. The aim of this study was to investigate the effects of ammonia stress on the pathogenicity of Shewanella spp. in Oreochromis niloticus. First, a 96-hour static test was used to determine the median lethal concentration (LC) of unionized ammonia to Nile tilapia. After 96 h of exposure, the Un-ionized ammonia (UIA) LC was estimated to be 4.26 mg/L. Second, an experiment was conducted to test the effect of unionized ammonia stress on the pathogenicity of Shewanella spp. in O. niloticus for 30 days. A study involved 180 fish divided into six groups, with the first group serving as a control. The second group (AMN1/10) and the third group (AMN1/20) were not challenged and were exposed to 1/10 (0.42 mg/L) and 1/20 (0.21 mg/L) of the 96-hour LC of UIA, respectively. Then 0.2 mL (0.14 × 10) of Shewanella spp. was intraperitoneally injected into the fourth (SH), fifth (SH + AMN1/10), and sixth (SH + AMN1/20) groups, which were subjected to 0, 1/10 (0.42 mg/L), and 1/20 (0.21 mg/L) of the 96-hour LC of UIA, respectively. The survival rate, hematological indices, immunological parameters, and antioxidant activity of the fish significantly decreased when they were exposed to ammonia and Shewanella infection separately or together. Histopathological changes were also observed in the kidney and liver. Furthermore, both individual and combined exposures significantly altered renal and hepatic function, with notable increases in glucose and cortisol levels, as well as in the expression of proinflammatory cytokine genes (TNF-α and IL-1ß). However, the detrimental effects of co-exposure to ammonia stress and Shewanella infection were greater than those of separate exposures. As a result, we may say that increased ammonia concentrations enhance the infection of Shewanella spp. These findings could contribute to a better understanding of Shewanella infection in Nile tilapia. - Source: PubMed
Publication date: 2024/07/18
Reda Rasha MEl-Murr AbdelhakeemAbdel-Basset Nehal AMetwally Mohamed M MIbrahim Rowida E