DHX58 antibody - N-terminal region (ARP36500_P050)
- Known as:
- DHX58 (anti-) - N-terminal region (ARP36500_P050)
- Catalog number:
- arp36500_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- DHX58 antibody - N-terminal region (ARP36500_P050)
Related genes to: DHX58 antibody - N-terminal region (ARP36500_P050)
- Gene:
- DHX58 NIH gene
- Name:
- DExH-box helicase 58
- Previous symbol:
- -
- Synonyms:
- LGP2, D11LGP2
- Chromosome:
- 17q21.2
- Locus Type:
- gene with protein product
- Date approved:
- 2007-06-20
- Date modifiied:
- 2016-09-27
Related products to: DHX58 antibody - N-terminal region (ARP36500_P050)
Related articles to: DHX58 antibody - N-terminal region (ARP36500_P050)
- Interleukin-27 (IL-27) is an anti-HIV cytokine that induces 14 novel microRNAs (miRNAs) in primary CD4(+) T cells. We previously reported that transfection of two of these miRNA mimics, miRTC10 and miRTC14 into human primary macrophages, differentially induced interferon (IFN)-α2, -α8, -α13, and -λ1 expression. However, the mechanism underlying this activation remains unclear. Here, we demonstrate that miRTC14 does not directly target canonical IFN-regulatory genes but instead engages with pyruvate carboxylase (PC) and laboratory of genetics and physiology-2 (LGP2/DHX58) as direct binding partner proteins. Functional analysis revealed that miRTC14 transfection induces IFN expression by more than 100-fold (p<0.001), whereas PC or LGP2 depletion by siRNAs markedly attenuated this response (50-100-fold reduction, p<0.01). Reconstitution of PC and LGP2 in deficient HEK293 cells restored miRTC14-driven IFN induction. Notably, IFN activation depended on sequence features at the duplex termini. However, PC-miRTC14-LGP2 axis activates TBK1-dependent phosphorylation of IRF3/7, similarly to canonical RNA sensors (RIG-I/MDA5), but this process induced differential IFN subtype. These findings establish PC as a miRNA-binding protein and define a previously unrecognized RNA-sensing mechanism, linking metabolic enzymes to RNA sequence-dependent innate immunity. - Source: PubMed
Publication date: 2026/08/05
Kariyawasam UdeshikaGoswami SuranjanaHao MingWiscovitch-Russo RosanaChen QianYang JunQu JuMarquez MayraSui HongyanChang WeizhongImamichi Tomozumi - Immune and stress responses are closely interconnected, with glucocorticoids modulating neutrophil number and activity, and cytokines influencing stress response. Neutrophils, due to their role as primary responders to infection, high sensitivity to glucocorticoid fluctuations, and critical involvement in gut microbiota homeostasis, were selected as the central focus of this study. We examined how antibiotic-induced dysbiosis affects the hypothalamus-pituitary-interrenal (HPI) axis and stress-related neutrophil dynamics in common carp (Cyprinus carpio L.). We analyzed the expression of stress-related genes and characterized neutrophil maturation and function within the hematopoietic niche. Dysbiotic fish exposed to acute stress exhibited significantly elevated cortisol levels compared to stressed fish with intact microbiota. Notably, even non-stressed dysbiotic fish showed increased cortisol level, indicating that microbiota disruption alone impairs HPI axis regulation. Antibiotic-treated and stressed fish displayed upregulation of il1β, gcsfr, cxcl8_l2, and cxcr1, suggesting enhanced granulopoiesis and neutrophil mobilization. However, systemic neutrophilia was attenuated in dysbiotic fish regardless of stress exposure. Transcriptomic profiling of neutrophils from dysbiotic, stressed fish revealed downregulation of mpx and cxcr4, and upregulation of mmp9, mhc1, trb, nlrp12, dhx58, irf3, irf7 and stat1, indicating altered maturation, increased migratory potential, and possible neutrophil-T cell interactions. In contrast, stressed fish with intact microbiota exhibited anti-apoptotic signatures and suppression of antiviral pathways. Across all stressed and dysbiotic groups, neutrophil phagocytic activity was significantly reduced. These findings underscore the pivotal role of gut microbiota in modulating stress responses, neutrophil development and trafficking, and immune function in vertebrates. - Source: PubMed
Publication date: 2026/07/26
Klak KatarzynaMaciuszek MagdalenaKralka JakubPecio AnnaNowak Barbara FKonstantinidis IoannisGalindo-Villegas JorgeChadzinska Magdalena - Nervous necrosis virus (NNV) is a major viral pathogen that causes viral nervous necrosis in a wide range of fish species and poses a serious threat to global aquaculture. However, the antiviral responses of mandarin fish (Siniperca chuatsi) to NNV infection remain poorly understood. In this study, we investigated host antiviral responses using Chinese perch brain (CPB) cell line and in vivo infection models. Transcriptome sequencing of red-spotted grouper nervous necrosis virus (RGNNV)-infected CPB cells revealed extensive transcriptional reprogramming, with numerous interferon-stimulated genes (ISGs) significantly upregulated. Comparative transcriptomic analysis across multiple teleost species identified a conserved set of ISGs induced during RGNNV infection, including CMPK2, RSAD2, DHX58, IRF3, and EPSTI1. Among these genes, CMPK2 showed strong induction in both infected CPB cells and mandarin fish brain tissues. Functional assays further demonstrated that CMPK2 overexpression significantly inhibited RGNNV replication in CPB cells and was accompanied by increased expression of several interferon-related genes. These findings provide the first functional evidence that CMPK2 restricts RGNNV infection in S. chuatsi. Our study highlights a conserved interferon-responsive antiviral module in teleost fish and identifies CMPK2 as a potential antiviral effector involved in host defense against RGNNV infection. - Source: PubMed
Publication date: 2026/07/17
Qin ChanxiaHuang JinlongLi LinmiaoLiang YingFeng HaiqingJiang HaiyingChen JiarongChen JinpingOu WeihaoYu Yepin - DExH-box helicase 58 (DHX58/LGP2) is an innate immune regulator with a previously uncharacterized role in oncology. This study evaluates the multifaceted role of the DHX58 gene, examining its pan-cancer expression, prognostic value, and therapeutic potential across various tumor types through an extensive pan-cancer bioinformatics analysis. - Source: PubMed
Publication date: 2026/06/01
Aziz Hussam SAl-Zaalan Ayoob Radhi - Influenza viruses continue to pose a global health threat, and available antiviral therapies are limited by resistance and reduced efficacy. Host-directed drugs such as MEK inhibitors have emerged as promising alternatives. Zapnometinib, a clinical-stage MEK inhibitor, has shown both antiviral and immunomodulatory activity. However, its impact on antigen presentation at the level of the HLA-I ligandome has not been investigated. - Source: PubMed
Publication date: 2026/05/26
Hamza HazemGhosh MichaelRammensee Hans-GeorgPlanz Oliver