KCTD4 antibody - N-terminal region (ARP35573_P050)
- Known as:
- KCTD4 (anti-) - N-terminal region (ARP35573_P050)
- Catalog number:
- arp35573_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- KCTD4 antibody - N-terminal region (ARP35573_P050)
Related genes to: KCTD4 antibody - N-terminal region (ARP35573_P050)
- Gene:
- KCTD4 NIH gene
- Name:
- potassium channel tetramerization domain containing 4
- Previous symbol:
- -
- Synonyms:
- bA321C24.3
- Chromosome:
- 13q14.12-q14.13
- Locus Type:
- gene with protein product
- Date approved:
- 2003-10-28
- Date modifiied:
- 2014-11-19
Related products to: KCTD4 antibody - N-terminal region (ARP35573_P050)
Related articles to: KCTD4 antibody - N-terminal region (ARP35573_P050)
- Heat stress represents a major limitation for livestock production systems, negatively affecting feed efficiency, animal health and welfare, and overall performance. In this context, the objective of this study was to identify genomic regions, candidate genes, biological pathways, and functional networks associated with dry matter intake (DMI) and residual feed intake (RFI) in Nellore cattle exposed to varying levels of thermal stress. The dataset comprised records from 22,838 animals, with genotypes available for 18,567 individuals. The data were collected during 296 feed efficiency trials between 2011 and 2023 across 21 Brazilian farms. Genome-wide association studies (GWAS) were performed using the single-step GBLUP (ssGBLUP) approach to account for genotype-by-environment (G×E) interactions in Nellore cattle. Environmental variation was modeled using the temperature-humidity index (THI) as the environmental gradient, with analyses stratified across three environmental gradients (EG): low (THI = 66), medium (THI = 74), and high (THI = 81). Fifty-one SNPs were significantly associated with RFI, including 27 shared across all three EGs, 10 exclusive to the low EG, one to the high EG, and 13 shared between the moderate and high EGs. These associations were mapped to 44 candidate genes, with 19 genes commonly identified across all EGs, including key candidates such as PIPOX, GTF2F2, KCTD4, MYO18A, and NFIA. For DMI, 136 significant SNPs were identified: 12 and 39 exclusive to the low and moderate EGs, respectively; 28 shared across all EGs; and 57 shared between the moderate and high EGs. These variants were linked to 58 candidate genes, of which 19 were common to all EGs, including NCAPG, LCORL, FAM13A, HERC3, CCND1, and FGF19. Gene network analyses revealed a clear reconfiguration of interaction structures across thermal gradients, particularly for RFI, where gene connectivity declined with increasing THI levels. For DMI, gene networks remained highly integrated, especially in the lowest THI level. Functional annotation highlighted both conserved and environment-specific regulatory architectures, involving key biological processes such as growth regulation, lipid and protein metabolism, intracellular signaling, stress response, and neuroendocrine control. These findings uncover the environmental sensitivity of RFI and DMI, highlight the complex and dynamic genomic basis of these traits under varying climatic conditions, and support the identification of candidate genes for genomic selection programs aiming to enhance climatic resilience in tropical beef cattle. - Source: PubMed
Publication date: 2026/01/05
Neto João B SilvaBrito Luiz FMota Lucio F MRodrigues Gustavo R DBaldi Fernando - Previously, we demonstrated that mitochondrial transplantation has beneficial effects in a polymicrobial sepsis model. However, the mechanism has not been fully investigated. Mitochondria have their own genes, and genomic changes in sepsis are an important issue in terms of pathophysiology, biomarkers, and therapeutic targets. To investigate the changes in transcriptomic features after mitochondrial transplantation in a polymicrobial sepsis model, we used a rat model of fecal slurry polymicrobial sepsis. Total RNA from splenocytes of sham-operated (SHAM, = 10), sepsis-induced (SEPSIS, = 7), and sepsis receiving mitochondrial transplantation (SEPSIS + MT, = 8) samples was extracted and we conducted a comparative transcriptome-wide analysis between three groups. We also confirmed these results with qPCR. In terms of percentage of mitochondrial mapped reads, the SEPSIS + MT group had a significantly higher mapping ratio than the others. and were identified as highly expressed in SEPSIS + MT compared with SEPSIS. Using SHAM expression levels as another control variable, we further identified six genes (, , , 7SK, SNORD94, and SNORA53) that were highly expressed after sepsis induction and observed that their expression levels were attenuated by mitochondrial transplantation. Changes in transcriptomic features were identified after mitochondrial transplantation in sepsis. This might provide a hint for exploring the mechanism of mitochondrial transplantation in sepsis. - Source: PubMed
Publication date: 2023/10/18
Kim SeongminNoh Ji HeonLee Min JiPark Ye JinKim Bo MiKim Yun-SeokHwang SangikPark ChungooKim Kyuseok - Increasing evidences suggest the important role of calcium homeostasis in hallmarks of cancer, but its function and regulatory network in metastasis remain unclear. A comprehensive investigation of key regulators in cancer metastasis is urgently needed. Transcriptome sequencing (RNA-seq) of primary esophageal squamous cell carcinoma (ESCC) and matched metastatic tissues and a series of gain/loss-of-function experiments identified potassium channel tetramerization domain containing 4 (KCTD4) as a driver of cancer metastasis. KCTD4 expression was found upregulated in metastatic ESCC. High KCTD4 expression is associated with poor prognosis in patients with ESCC and contributes to cancer metastasis and . Mechanistically, KCTD4 binds to CLIC1 and disrupts its dimerization, thus increasing intracellular Ca level to enhance NFATc1-dependent fibronectin transcription. KCTD4-induced fibronectin secretion activates fibroblasts in a paracrine manner, which in turn promotes cancer cell invasion MMP24 signaling as positive feedback. Furthermore, a lead compound K279-0738 significantly suppresses cancer metastasis by targeting the KCTD4‒CLIC1 interaction, providing a potential therapeutic strategy. Taken together, our study not only uncovers KCTD4 as a regulator of calcium homeostasis, but also reveals KCTD4/CLIC1-Ca-NFATc1-fibronectin signaling as a novel mechanism of cancer metastasis. These findings validate KCTD4 as a potential prognostic biomarker and therapeutic target for ESCC. - Source: PubMed
Publication date: 2023/07/17
Zheng CancanYu XiaomeiXu TaoyangLiu ZhichaoJiang ZhiliXu JiaojiaoYang JingZhang GuogengHe YanYang HanShi XingyuanLi ZhigangLiu JinbaoXu Wen Wen - Oligomerization endows proteins with some key properties such as extra-stabilization, long-range allosteric regulation(s), and partnerships not accessible to their monomeric counterparts. How oligomerization is achieved and preserved during evolution is a subject of remarkable scientific relevance. By exploiting the abilities of the machine-learning algorithms implemented in AlphaFold (AF) in predicting protein structures, herein, we report a comprehensive analysis of the structural states of functional oligomers of all members of the KCTD protein family. Interestingly, our approach led to the identification of reliable three-dimensional models for the pentameric states of KCNRG, KCTD6, KCTD4, KCTD7, KCTD9, and KCTD14 and possibly for KCTD11 and KCTD21 that are involved in key biological processes and that were previously uncharacterized from a structural point of view. Although for most of these proteins, the CTD domains lack any sequence similarity, they share some important structural features, such as a propeller-like structure with a central cavity delimited by five exposed and regular β-strands. Moreover, the structure of the related proteins KCTD7 and KCTD14, although pentameric, appears to be characterized by a different organization of the CTD region, with the five chains forming a circle-like structure with a large cavity. Our predictions also suggest that other members of the family, such as KCTD10, KCTD13, and TNFAIP1, present a strong propensity to assume dimeric states. Although the structures of the functional oligomers reported herein represent models that require additional validations, they provide a consistent and global view of KCTD protein oligomerization. - Source: PubMed
Publication date: 2022/11/01
Esposito LucianaBalasco NicoleVitagliano Luigi - A cell-free DNA (cfDNA) assay would be a promising approach to early cancer diagnosis, especially for patients with dense tissues. Consistent cfDNA signatures have been observed for many carcinogens. Recently, investigations of cfDNA as a reliable early detection bioassay have presented a powerful opportunity for detecting dense tissue screening complications early. We performed a prospective study to evaluate the potential of characterizing cfDNA as a central element in the early detection of dense tissue breast cancer (BC). Plasma samples were collected from 32 consenting subjects with dense tissue and positive mammograms, 20 with positive biopsies and 12 with negative biopsies. After screening and before biopsy, cfDNA was extracted, and whole-genome next-generation sequencing (NGS) was performed on all samples. Copy number alteration (CNA) and single nucleotide polymorphism (SNP)/insertion/deletion (Indel) analyses were performed to characterize cfDNA. In the positive-positive subjects (cases), a total of 5 CNAs overlapped with 5 previously reported BC-related oncogenes (KSR2, MAP2K4, MSI2, CANT1 and MSI2). In addition, 1 SNP was detected in KMT2C, a BC oncogene, and 9 others were detected in or near 10 genes (SERAC1, DAGLB, MACF1, NVL, FBXW4, FANK1, KCTD4, CAVIN1; ATP6V0A1 and ZBTB20-AS1) previously associated with non-BC cancers. For the positive-negative subjects (screening), 3 CNAs were detected in BC genes (ACVR2A, CUL3 and PIK3R1), and 5 SNPs were identified in 6 non-BC cancer genes (SNIP1, TBC1D10B, PANK1, PRKCA and RUNX2; SUPT3H). This study presents evidence of the potential of using cfDNA somatic variants as dense tissue BC biomarkers from a noninvasive liquid bioassay for early cancer detection. - Source: PubMed
Publication date: 2022/05/19
Barbirou MouadhMiller Amanda AGafni ErikMezlini AmelZidi AsmaBoley NathanTonellato Peter J