YBX1 antibody - middle region (ARP34396_P050)
- Known as:
- YBX1 (anti-) - middle region (ARP34396_P050)
- Catalog number:
- arp34396_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- YBX1 antibody - middle region (ARP34396_P050)
Related genes to: YBX1 antibody - middle region (ARP34396_P050)
- Gene:
- YBX1 NIH gene
- Name:
- Y-box binding protein 1
- Previous symbol:
- NSEP1
- Synonyms:
- YB-1, YB1, DBPB, NSEP-1, MDR-NF1, BP-8, CSDB, CSDA2
- Chromosome:
- 1p34.2
- Locus Type:
- gene with protein product
- Date approved:
- 1993-10-20
- Date modifiied:
- 2016-10-05
Related products to: YBX1 antibody - middle region (ARP34396_P050)
Related articles to: YBX1 antibody - middle region (ARP34396_P050)
- RNA modification is a widespread post-transcriptional regulatory process, of which 5-methylcytosine (m5C) is an important part of the RNA modification regulatory network. Ten-eleven translocation 2 (TET2), a demethylase of m5C, has been regarded as an important factor in a variety of diseases. However, the role of TET2 in diabetic nephropathy (DN) and its mechanism remain elusive. Here, we found that m5C modification was significantly increased in the kidney of diabetic mice, which was caused by decreased levels of TET2. Renal biopsy samples from patients diagnosed with type 2 DN, together with normal peritumoural tissues from renal carcinoma patients, were obtained and accompanied by corresponding clinical profiles. In the glomeruli of patients with DN, a pronounced downregulation of TET2 expression was observed. TET2 levels were significantly associated with podocyte markers and indicators of renal function, suggesting its potential relevance to DN progression. Functional studies revealed that overexpression of TET2 alleviated high glucose (HG)-induced podocyte damage by enhancing autophagy in both db/db mice models and cultured podocytes. Furthermore, the downstream molecular mechanisms were investigated by identifying m5C-methylated target transcripts Ras related GTP binding C (RRAGC) through m5C methylated RNA immunoprecipitation sequencing (MeRIP-seq). Mechanistically, TET2 attenuated HG-induced autophagy defects by decreasing the m5C level and mRNA stability of RRAGC, potentially involving the m5C reader Y-box binding protein 1 (YBX1). This work may open new avenues for exploring preventive and therapeutic strategies for DN. - Source: PubMed
Publication date: 2026/09/28
Ma YuHu ZiyunWang YukaiShi YuZhu WentingLiu XueqiWu Yonggui - Ferroptosis, a regulated form of cell death driven by lipid peroxide accumulation, is crucial in tumor development and controlled by Glutathione Peroxidase 4 (GPX4). Targeting ferroptosis offers a promising cancer therapy approach but remains challenging. Here we show that Protein Arginine Methyltransferase 1 (PRMT1) is highly expressed in breast cancer and forms a transcriptional activation complex with Y-box binding protein 1 (YBX1). This complex binds the GPX4 promoter, suppressing ferroptosis and promoting breast cancer growth and metastasis. YBX1 recruits PRMT1 independently of its enzymatic activity, while PRMT1's methyltransferase function activates transcription via H4R3me2a modification. Combined treatment with MS023 (a type I PRMT inhibitor targeting PRMT1) and SU056 (a YBX1 inhibitor) synergistically decreases GPX4 expression, enhances ferroptosis, and suppresses tumor growth. These findings establish the PRMT1-YBX1-GPX4 axis as a previously unrecognized transcriptional regulatory module and a promising therapeutic target for breast cancer. - Source: PubMed
Publication date: 2026/09/22
Li YuYuan BaowenLiu JiaxiangWang XinZhang JingyaoHu TingMa TianyuZhang DieWang YinuoZhang JingjingGao JiayiGao TianyangHao XinhuiGuo ChangYan DongHuang WeiWang Yan - Certain circular RNAs (circRNAs) are selectively enriched in extracellular vesicles (EVs), in which they contribute to intercellular communication and represent promising biomarkers, yet the sequence determinants of their sorting remain unclear. Existing computational predictors are optimized mainly for linear RNAs and rarely address circRNA localization into EVs. Here we introduce circExor, the first framework specifically designed for circRNA EV localization. We curate a dedicated benchmark data set of 2102 circRNAs and implement a variable-length end-to-end concatenation strategy together with -mer frequency encoding to accommodate circular topology, long sequence length, and length heterogeneity. Using a tree-based classifier, circExor achieves superior performance compared with RNAlocate-v3 and ExoGRU, reaching an AUROC of 0.743 on the internal test set and an average AUROC of 0.680 on the held-out test set. SHAP-based analysis, sequence perturbation analysis, motif mapping, and cell-based experimental validation support the predicted EV tendency and identify YBX1, HNRNPK, HNRNPL, and NOVA2 as candidate RBPs potentially associated with circRNA sorting. CircExor therefore provides a predictive and interpretable framework that links in silico modeling to mechanistic hypotheses, and supports biomarker discovery and candidate prioritization for downstream studies of EV-associated circRNAs. - Source: PubMed
Publication date: 2026/10/01
Zhang YusaLu HanboBao PengfeiWang AnhaoLyu XiaohongZhou YidongShen SongjieLu Zhi John - Spermatogonial stem cells (SSCs) are required for initiating and maintaining normal spermatogenesis, and notably, they have significant applications in both reproductive and regenerative medicine owing to their great plasticity with de-differentiation and trans-differentiation potentials. Nevertheless, molecular mechanisms regulating human SSC fate determinations and male fertility remain elusive. Here we report for the first time that structural maintenance of chromosomes 1A (SMC1A) interacts with RNA-binding protein YBX1 to control fate decisions of human SSCs and an association exists between SMC1A dysfunction with male infertility. SMC1A/YBX1 complex stabilizes high mobility group AT-hook 2 (HMGA2) mRNA in an mC modification-dependent manner to activate the G1/S phase transition, which ultimately mediates human SSC self-renewal and apoptosis. Transplantation of SSCs into the seminiferous tubules of recipient mice without male germ cells reveals that Smc1a silencing impairs SSC colonization and causes differentiation defect. Notably, Smc1a-conditional knockout (Smc1a-cKO) mice assume spermatogenesis disorder and obviously compromise male fertility. Single-cell transcriptomics demonstrates that Smc1a-cKO mice exhibit the downregulation of genes associated with mitosis, meiosis, and mC regulation. Significantly, whole-exome sequencing (WES) of 2028 non-obstructive azoospermia (NOA) patients identify two deleterious SMC1A variants and one deleterious HMGA2 variant. SMC1A and HMGA2 expression levels were remarkably decreased in testicular tissues from NOA patients with spermatogenic failure compared to obstructive azoospermia (OA) patients with normal spermatogenesis. Collectively, our findings highlight the critical roles and a novel mechanism by the SMC1A/YBX1/HMGA2 axis in regulating human SSC fate decisions and male fertility via an mC modification. This study is thus of the utmost significance because it would provide novel molecular mechanisms underlying fate determinations of adult stem cells and human spermatogenesis and it could offer network targets for gene targeting male infertility and other diseases. - Source: PubMed
Publication date: 2026/09/15
Liu WeiDu LiZhu YannaXu YuxuanZhou DaiLiu BangGao JunCui YinghongChen WeiLi ChunyunChen YongzheJiang FenHe Zuping - Skin aging is characterized by epidermal atrophy, reduced keratinocyte proliferation, and the accumulation of senescent cells that sustain a chronic, low-grade inflammatory secretome known as the senescence-associated secretory phenotype (SASP). We previously showed that Y-box binding protein 1 (YBX1) limits keratinocyte senescence in human epidermis by acting as a translational repressor of SASP chemokines, including CXCL1 and IL8. How this brake is regulated during skin aging, however, remains undefined. Here we demonstrate that, although total YBX1 protein is reduced in keratinocytes from aged human epidermis, the fraction of phosphorylated YBX1 (pYBX1) is increased relative to young donors, resulting in an elevated pYBX1/total YBX1 ratio that correlates with chronological age. Because pYBX1 is predominantly nuclear, whereas unphosphorylated YBX1 is cytoplasmic, this shift is predicted to deplete the cytoplasmic pool available for translational repression of CXCL1 and IL8. Consistent with this model, treatment of immortalized (Ker-CT) and primary human keratinocytes (HK) with the PI3K inhibitors PI-103 and GDC-0941 suppressed YBX1 phosphorylation without altering total YBX1 abundance, thus retaining YBX1 in the cytoplasm. This allows cytoplasmic YBX1 to re-engage and repress the translation of and mRNAs, lower chemokine secretion, and reduce the fraction of senescent keratinocytes. Moreover, conditioned medium from PI-103- or GDC-0941-treated cells sufficiently decreased senescence in recipient cells, consistent with a paracrine, secretome-mediated effect. Together, these findings support an association between YBX1 phosphorylation and SASP chemokine output in aging human keratinocytes and nominate YBX1-directed modulation as a candidate strategy for selectively attenuating senescence-associated secretory phenotypes in intrinsic skin aging. - Source: PubMed
Publication date: 2026/09/01
Japranata Valdi VenLiu MichelleBoncimino FabianaDi Cicco EmeryPalumbo SaraTodorova KristinaGantumur EnkhtuulSol StefanoMandinova Anna