KIF2B antibody - middle region (ARP34082_P050)
- Known as:
- KIF2B (anti-) - middle region (ARP34082_P050)
- Catalog number:
- arp34082_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- KIF2B antibody - middle region (ARP34082_P050)
Related genes to: KIF2B antibody - middle region (ARP34082_P050)
- Gene:
- KIF2B NIH gene
- Name:
- kinesin family member 2B
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 17q22
- Locus Type:
- gene with protein product
- Date approved:
- 2005-02-07
- Date modifiied:
- 2014-08-12
Related products to: KIF2B antibody - middle region (ARP34082_P050)
Related articles to: KIF2B antibody - middle region (ARP34082_P050)
- Rapid chromosome movements during meiotic prophase are critical for homologous chromosome pairing and proper meiotic progression. These movements are generated by the cytoskeleton and are transmitted to the telomeres via the LINC complex, yet the cytoplasmic components that generate these forces remain poorly defined. In a proteomic screen, we identified candidates of microtubule-associated motor proteins in mouse spermatocytes. Among these, KIF5B and KIF2B emerged as specific interactors of the LINC complex through co-immunoprecipitation and yeast two-hybrid assays using KASH5 as bait. Total internal reflection fluorescence microscopy and microtubule sedimentation assays performed with recombinant proteins suggest direct interaction between purified KASH5 and KIF5B on microtubules. Mapping KIF5B-binding surface of KASH5, revealed that KASH5 N-terminal EF-hand domains mediate the interaction. Further, in vivo KIF5B-KASH5 interaction and KIF5B role in RPMs is evidenced as (1) KIF5B is recruited by KASH5-SUN1 to the nuclear envelope in a cultured somatic cell model, (2) KIF5B is telomere associated and colocalizes with KASH5 and microtubules associated to the nuclear envelope in mouse spermatocytes, (3) inhibition of kinesins reduces telomere-led chromosome motions. Altogether, our findings identify kinesins, particularly KIF5B, as a previously unrecognized component of the force-generating machinery that drives chromosome movement during meiotic prophase I, acting through KASH5 as a specific nuclear membrane adaptor. - Source: PubMed
Publication date: 2025/05/29
Ditamo YWanjing SPreviato LGillies J PCarbajal AKinter MDeSantis M EBisig C GPezza R J - In gametogenesis, microtubules undergo dramatic changes known as microtubule dynamics, and which is important for fertility both male and female. In spermatogenesis, spindle microtubule dynamics occur during meiosis and manchette microtubule dynamics occur in elongated spermatids. In oogenesis, spindle microtubule dynamics occur during meiosis. The microtubule depolymerization protein kinesin-13 family (KIF2A, KIF2B, and KIF2C) plays an important role in microtubule dynamics, and KIF2C is a well-known microtubule depolymerization factor in mitosis. Although the function of KIF2C in mitosis has been extensively studied, its role in meiosis remains unclear. Additionally, the role of microtubule dynamics in manchette formation remains unclear. We generated germ cell-specific conditional knockout ( cKO) mice to elucidate KIF2C function in germ cells. cKO male mice showed chromosomal misalignment at meiotic metaphase, abnormal manchette morphology and delayed manchette disassembly, which led to a significant increase in apoptosis. Furthermore, cKO male mice were completely infertile. Therefore, KIF2C plays an important role in chromosomal alignment in male meiosis and in manchette dynamics in elongated spermatids. In contrast, cKO female mice were sufficiently fertile, and only minor defects were observed in chromosome alignment in meiosis. This study demonstrates, for the first time, that KIF2C is important for microtubule dynamics of spermatogenesis to achieve male fertility, but not for female fertility. - Source: PubMed
Publication date: 2025/03/27
Harima RyuaKishinami MayuHara KenshiroTanemura Kentaro - Medulloblastoma is one of the most common malignant tumors of the central nervous system in children. Although KIF2B was reported as an oncogene in several malignant tumor types, its role in medulloblastoma has not been studied so far. The PCR results of our study showed that KIF26B is highly expressed in medulloblastoma, and its high expression is associated with a high clinical stage. Knockdown the expression of KIF26B could significantly impair the proliferation and migration of medulloblastoma cells. KIF26B promotes the malignant progression of medulloblastoma by affecting the expression of phosphorylation of key proteins in the PI3K/AKT signaling pathway. With the help of 740 Y-P, activating the pi3k signaling pathway can partially rescue the phenotype. Therefore, our experimental results suggest that KIF26B is a potential target for medulloblastoma. - Source: PubMed
Publication date: 2022/07/12
Liu YajunZhang XiPan RuihanLiang XiaolongLiu QichangYang ChaoLi Xu - Male infertility is a multifaceted disorder affecting approximately 50% of male partners in infertile couples. Over the years, male infertility has been diagnosed mainly through semen analysis, hormone evaluations, medical records and physical examinations, which of course are fundamental, but yet inefficient, because 30% of male infertility cases remain idiopathic. This dilemmatic status of the unknown needs to be addressed with more sophisticated and result-driven technologies and/or techniques. Genetic alterations have been linked with male infertility, thereby unveiling the practicality of investigating this disorder from the "omics" perspective. Omics aims at analyzing the structure and functions of a whole constituent of a given biological function at different levels, including the molecular gene level (genomics), transcript level (transcriptomics), protein level (proteomics) and metabolites level (metabolomics). In the current study, an overview of the four branches of omics and their roles in male infertility are briefly discussed; the potential usefulness of assessing transcriptomic data to understand this pathology is also elucidated. After assessing the publicly obtainable transcriptomic data for datasets on male infertility, a total of 1385 datasets were retrieved, of which 10 datasets met the inclusion criteria and were used for further analysis. These datasets were classified into groups according to the disease or cause of male infertility. The groups include non-obstructive azoospermia (NOA), obstructive azoospermia (OA), non-obstructive and obstructive azoospermia (NOA and OA), spermatogenic dysfunction, sperm dysfunction, and Y chromosome microdeletion. Findings revealed that 8 genes () were commonly differentially expressed between all disease groups. Likewise, 56 genes were common between NOA versus NOA and OA (). These genes, particularly the above-mentioned 8 genes, are involved in diverse biological processes such as germ cell development, spermatid development, spermatid differentiation, regulation of proteolysis, spermatogenesis and metabolic processes. Owing to the stage-specific expression of these genes, any mal-expression can ultimately lead to male infertility. Therefore, currently available data on all branches of omics relating to male fertility can be used to identify biomarkers for diagnosing male infertility, which can potentially help in unravelling some idiopathic cases. - Source: PubMed
Publication date: 2022/02/14
Omolaoye Temidayo SOmolaoye Victor AKandasamy Richard KHachim Mahmood YaseenDu Plessis Stefan S - Microtubule depolymerases of the kinesin-13 family play important roles in various cellular processes and are frequently overexpressed in different cancer types. Despite the importance of their correct abundance, remarkably little is known about how their levels are regulated in cells. Using comprehensive screening on protein microarrays, we identified 161 candidate substrates of the multi-subunit ubiquitin E3 ligase SCF , including the kinesin-13 member Kif2c/MCAK. In vitro reconstitution assays demonstrate that MCAK and its closely related orthologs Kif2a and Kif2b become efficiently polyubiquitylated by neddylated SCF and Cdc34, without requiring preceding modifications. In cells, SCF targets MCAK for proteasomal degradation predominantly during G . While this seems largely dispensable for mitotic progression, loss of Fbxw5 leads to increased MCAK levels at basal bodies and impairs ciliogenesis in the following G /G , which can be rescued by concomitant knockdown of MCAK, Kif2a or Kif2b. We thus propose a novel regulatory event of ciliogenesis that begins already within the G phase of the preceding cell cycle. - Source: PubMed
Publication date: 2021/08/09
Schweiggert JörgHabeck GregorHess SandraMikus FelixBeloshistov RomanMeese KlausHata ShojiKnobeloch Klaus-PeterMelchior Frauke