PARP8 antibody - C-terminal region (ARP33771_P050)
- Known as:
- PARP8 (anti-) - C-terminal region (ARP33771_P050)
- Catalog number:
- arp33771_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- PARP8 antibody - C-terminal region (ARP33771_P050)
Related genes to: PARP8 antibody - C-terminal region (ARP33771_P050)
- Gene:
- PARP8 NIH gene
- Name:
- poly(ADP-ribose) polymerase family member 8
- Previous symbol:
- -
- Synonyms:
- FLJ21308, pART16
- Chromosome:
- 5q11.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-08-25
- Date modifiied:
- 2016-10-05
Related products to: PARP8 antibody - C-terminal region (ARP33771_P050)
Related articles to: PARP8 antibody - C-terminal region (ARP33771_P050)
- Renal fibrosis (RF) represents a major pathological outcome of chronic kidney disease, currently accompanied by extremely limited therapeutic strategies. To decipher key cellular and molecular drivers, we integrated single-cell and bulk transcriptomic profiles for comprehensive analysis. Based on the RF-related single-cell and bulk transcriptomic data, key cell subtypes were identified through Scissor analysis, custom signature matrix construction via CIBERSORTx, and Weighted Gene Co-Expression Network Analysis (WGCNA). Subsequently, key subtype-related biomarkers were identified through the expression analysis, and functional enrichment analysis for biomarkers was conducted to elucidate the potential mechanisms by which biomarkers regulate RF. Through comprehensive profiling, thick ascending limb (TAL) cells were predominant and displayed marked heterogeneity in renal fibrosis (RF), with cortical TAL (CTAL) and adaptive TAL (aTAL) identified as principal subtypes. A set of candidate biomarkers was identified. Quantitative polymerase chain reaction (qPCR) validation in mouse models confirmed aberrant expression of these biomarkers, with STAT1 and PARP8 upregulated and HS6ST2, PTGER3, and TMEM207 downregulated in RF. Furthermore, functional enrichment analyses indicated that these biomarkers were associated with pathways underlying metabolic reprogramming and immune perturbation. Our study implicates CTAL and aTAL as central cellular players in RF and identifies their associated biomarkers. These experimentally validated biomarkers provide novel targets and repurposing opportunities for RF therapeutic intervention. - Source: PubMed
Publication date: 2026/02/16
Wang HengpingZhang YuanLi JialeFu YingWang Huiyan - This study investigated the genetic and functional association between PARP8 gene and high/extreme myopia (HM/EM) in a Han Chinese population. Genotyping of five SNPs (rs11954386, rs2404958, rs282544, rs32396, and rs27243) in PARP8 across 1768 participants revealed a significant association with HM/EM. Notably, rs27243 (situated in the 3'-UTR region of PARP8) exhibited a robust linkage. Experimental validations confirmed that the rs27243 A allele created a binding site for hsa-miR-410-3p, which led to the downregulation of PARP8 expression. Furthermore, diminished PARP8 and elevated hsa-miR-410-3p levels were detected in patients, along with reduced PARP8 expression in form-deprivation myopia mouse models, suggesting that PARP8 and hsa-miR-410-3p are potential risk factors or biomarkers for HM/EM. Collectively, these findings establish PARP8 as a novel susceptibility gene for HM/EM and reveal hsa-miR-410-3p-mediated post-transcriptional regulation of PARP8 as a pivotal mechanism in myopia pathogenesis. - Source: PubMed
Publication date: 2025/11/06
Wan YiweiShui BingyueYang YuheGong YukaiYang YinZou LiangZhou LinJiang Lingxi - Ulcerative colitis (UC) is a chronic nonspecific inflammatory intestinal disease affecting the mucosa and submucosa, characterized by continuous and diffuse active inflammation. However, its underlying pathogenesis remains unclear. - Source: PubMed
Publication date: 2025/09/30
Chen ZepengWang XingchenXiao ChangfangCao Yongqing - How transcriptional programs coordinate the transition from neural progenitors to lineage-committed neurons in the spinal cord remains poorly understood. While much is known about transcription factors acting in the proliferative and differentiated zones, the role of intermediate zone (IZ) factors during lineage specification is less clear. Here, we investigate the function of SCRATCH2 (SCRT2), expressed in the postmitotic cells of the IZ, during dorsal interneuron differentiation. Overexpression of SCRT2 in vivo reduced the number of ISLET1+ dorsal interneurons. Chromatin profiling revealed that SCRT2 primarily binds to intergenic, transcriptionally inactive regions near neurogenic genes. Among these, we identified a conserved regulatory element, ECR4, located between ISLET1 and PARP8. Functional assays showed that ECR4 drives neural transcription and is composed of two subregions: ECR4B, an enhancer activated by ISLET1 and POU4F1, and ECR4A, which contains SCRT2 binding motifs and mediates transcriptional repression. Mutation of the vCES-box, a predicted SCRT2-binding motif within ECR4A, abolished repression, confirming a repressive regulatory interaction. Together, these data support a model in which SCRT2 represses ISLET1 through ECR4 to modulate dI3 lineage specification. These findings identify a novel regulatory mechanism linking intermediate zone transcriptional repression to dorsal interneuron development in the spinal cord. - Source: PubMed
Publication date: 2025/08/14
Botezelli Vitória SKanno Tatiane YLiau Ee ShanGoes Carolina Pde La Cruz Anticona ShirleyAzambuja Ana PaulaSimoes-Costa MarcosYan C Y Irene - Uveal melanoma (UM) is a primary intraocular malignancy with a high-risk of metastasis. Currently, there are no studies that construct prognostic models based on immune-related molecular subtypes. We performed unsupervised clustering of immune cell infiltration matrices based on the the cancer genome atlas-uveal melanoma (TCGA-UVM) dataset, identifying 2 clusters with distinct expression patterns of immune checkpoint and immune activation related genes. gene ontology/Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that genes in the immune-related gene modules identified by WGCNA were associated with immune activity and cell proliferation. Using Cox and LASSO regression analysis based on the immune-related gene modules to construct a prognostic model. The prognostic model was validated in external datasets of Gene Expression Omnibus (GEO) database. We constructed a prognostic model comprising genes S100A4, KCNIP3, PARP8, ORAI2, MMP12, ISG20, MMP9, and CEBPB. The model stratified patients into high and low-risk groups, with the high-risk group showing poorer prognosis. The model's predictive accuracy was validated with the AUC values exceeding 0.8 for 1-year, 3-year, and 5-year survival rates and confirmed in external datasets GSE22138 and GSE84976. Differential gene analysis between risk groups highlighted the association with immune response and cell proliferation functions. The CEBPB gene in the model played crucial roles in tumor progression. In vivo and in vitro experiments validated the impact of CEBPB on the biological functions of UM. Experiments in UM cells revealed that CEBPB promoted cell proliferation, migration and invasion, as well as suppressing apoptosis, indicating its potential as a therapeutic target. The prognostic model based on 8 immune-related genes effectively predicted the survival outcomes of UM. Knockdown of CEBPB significantly reduced the progression of UM, suggesting that it could be a potential therapeutic target for UM. - Source: PubMed
Tao YulinPeng YiruiZhu HaiboXiong MinqiZhou QiongOuyang Jun