KNTC1 antibody - N-terminal region (ARP33708_P050)
- Known as:
- KNTC1 (anti-) - N-terminal region (ARP33708_P050)
- Catalog number:
- arp33708_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- KNTC1 antibody - N-terminal region (ARP33708_P050)
Related genes to: KNTC1 antibody - N-terminal region (ARP33708_P050)
- Gene:
- KNTC1 NIH gene
- Name:
- kinetochore associated 1
- Previous symbol:
- -
- Synonyms:
- KIAA0166, ROD
- Chromosome:
- 12q24.31
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-19
- Date modifiied:
- 2015-08-28
- Gene:
- ZW10 NIH gene
- Name:
- zw10 kinetochore protein
- Previous symbol:
- -
- Synonyms:
- KNTC1AP
- Chromosome:
- 11q23.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-05-11
- Date modifiied:
- 2016-10-05
- Gene:
- ZWILCH NIH gene
- Name:
- zwilch kinetochore protein
- Previous symbol:
- -
- Synonyms:
- FLJ10036, KNTC1AP
- Chromosome:
- 15q22.31
- Locus Type:
- gene with protein product
- Date approved:
- 2005-07-25
- Date modifiied:
- 2014-11-19
Related products to: KNTC1 antibody - N-terminal region (ARP33708_P050)
Related articles to: KNTC1 antibody - N-terminal region (ARP33708_P050)
- Kinetochore associated 1 (KNTC1) encodes a kinetochore component in Rod-Zwilch-ZW10 (RZZ) complex which is essential for the segregation of sister chromatids during mitosis and participates in the spindle checkpoint. Recent research demonstrated that kinetochore proteins may be potential biomarkers and may contribute to the development of human malignancies. Our immunohistochemistry experiment showed that KNTC1 was highly expressed in hepatocellular carcinoma (HCC) tissues and correlated with terrible prognosis, indicating that KNTC1 acts a pivotal role in HCC development. Furthermore, lentivirus delivered short hairpin RNA (shRNA) KNTC1 (Lv-shKNTC1) was applied to infect BEL-7404 and SK-HEP-1 to identify roles of KNTC1 on HCC. Lv-shKNTC1 cells showed reduced proliferation ability, increased apoptosis and decreased migration ability. In vivo experiments suggested that xenografts grow significantly slower upon the silencing of KNTC1. Mechanistically, the protein levels of PIK3CA, p-Akt, CCND1, CDK6 are all down-regulated in Lv-KNTC1 cells and the Lv-shKNTC1 tumor tissues of nude mice. Therefore, KNTC1 may affect the biological activity of HCC cells through PI3K/Akt signaling pathway. Further studies revealed that ZW10 is a pivotal protein that participates in KNTC1-induced regulation of PI3K/Akt signaling pathway. In summary, the key finding of this report highlighted the significance of KNTC1 in tumor regression of HCC, demonstrating KNTC1 as an innovative target for adjuvant treatment of HCC. - Source: PubMed
Publication date: 2022/10/21
Tong HuiLiu XiaohuiPeng ChenghongShen BaiyongZhu Zhecheng - Faithful chromosome segregation depends on the ability of sister kinetochores to attach to spindle microtubules. The outer layer of kinetochores transiently expands in early mitosis to form a fibrous corona, and compacts following microtubule capture. Here we show that the dynein adaptor Spindly and the RZZ (ROD-Zwilch-ZW10) complex drive kinetochore expansion in a dynein-independent manner. C-terminal farnesylation and MPS1 kinase activity cause conformational changes of Spindly that promote oligomerization of RZZ-Spindly complexes into a filamentous meshwork in cells and in vitro. Concurrent with kinetochore expansion, Spindly potentiates kinetochore compaction by recruiting dynein via three conserved short linear motifs. Expanded kinetochores unable to compact engage in extensive, long-lived lateral microtubule interactions that persist to metaphase, and result in merotelic attachments and chromosome segregation errors in anaphase. Thus, dynamic kinetochore size regulation in mitosis is coordinated by a single, Spindly-based mechanism that promotes initial microtubule capture and subsequent correct maturation of attachments. - Source: PubMed
Publication date: 2018/06/18
Sacristan CarlosAhmad Misbha Ud DinKeller JennyFermie JobGroenewold VincentTromer EelcoFish AlexanderMelero RobertoCarazo José MaríaKlumperman JudithMusacchio AndreaPerrakis AnastassisKops Geert Jpl - The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by delaying anaphase onset in response to unattached kinetochores. Checkpoint signalling requires the kinetochore localization of the Mad1-Mad2 complex that in more complex eukaryotes depends on the Rod-Zwilch-ZW10 (RZZ) complex. The kinetochore protein Zwint has been proposed to be the kinetochore receptor for RZZ, but here we show that Bub1 and not Zwint is required for RZZ recruitment. We find that the middle region of Bub1 encompassing a domain essential for SAC signalling contributes to RZZ localization. In addition, we show that a distinct region in Bub1 mediates kinetochore localization of BubR1 through direct binding, but surprisingly removal of this region increases checkpoint strength. Our work thus uncovers how Bub1 coordinates checkpoint signalling by distinct domains for RZZ and BubR1 recruitment and suggests that Bub1 localizes antagonistic checkpoint activities. - Source: PubMed
Publication date: 2015/06/02
Zhang GangLischetti TizianaHayward Daniel GNilsson Jakob - The Zeste-White 10 (ZW10) and Rough Deal (ROD) proteins are part of a complex necessary for accurate chromosome segregation. This complex recruits cytoplasmic dynein to the kinetochore and participates in the spindle checkpoint. We used immunoaffinity chromatography and mass spectroscopy to identify the Drosophila proteins in this complex. We found that the complex contains an additional protein we name Zwilch. Zwilch localizes to kinetochores and kinetochore microtubules in a manner identical to ZW10 and ROD. We have also isolated a zwilch mutant, which exhibits the same mitotic phenotypes associated with zw10 and rod mutations: lagging chromosomes at anaphase and precocious sister chromatid separation upon activation of the spindle checkpoint. Zwilch's role within the context of this complex is evolutionarily conserved. The human Zwilch protein (hZwilch) coimmunoprecipitates with hZW10 and hROD from HeLa cell extracts and localizes to the kinetochores at prometaphase. Finally, we discuss immunoaffinity chromatography results that suggest the existence of a weak interaction between the ZW10/ROD/Zwilch complex and the kinesin-like kinetochore component CENP-meta. - Source: PubMed
Williams Byron CLi ZeXiaoLiu SongtaoWilliams Erika VLeung GarmayYen Tim JGoldberg Michael L