TSC22D2 antibody - C-terminal region (ARP33697_T100)
- Known as:
- TSC22D2 (anti-) - C-terminal region (ARP33697_T100)
- Catalog number:
- arp33697_t100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- TSC22D2 antibody - C-terminal region (ARP33697_T100)
Related genes to: TSC22D2 antibody - C-terminal region (ARP33697_T100)
- Gene:
- TSC22D2 NIH gene
- Name:
- TSC22 domain family member 2
- Previous symbol:
- -
- Synonyms:
- KIAA0669, TILZ4a, TILZ4b, TILZ4c
- Chromosome:
- 3q25.1
- Locus Type:
- gene with protein product
- Date approved:
- 2005-03-01
- Date modifiied:
- 2015-11-18
Related products to: TSC22D2 antibody - C-terminal region (ARP33697_T100)
Related articles to: TSC22D2 antibody - C-terminal region (ARP33697_T100)
- WNK family kinases are regulated by osmotic stress and control ion homeostasis by activating SPAK and OXSR1 kinases. Using a proximity labeling approach, we found that osmotic stress promotes the association of WNK1 with the NRBP1 pseudokinase and TSC22D2/4 adaptor proteins, results that are confirmed by immunoprecipitation, mass spectrometry, and immunoblotting studies. NRBP1 pseudokinase is closely related to WNK isoforms and contains a RΦ-motif-binding conserved C-terminal (CCT) domain, like the CCT domains in WNKs, SPAK, and OXSR1. Knockdown or knockout of NRBP1 markedly inhibited basal as well as sorbitol-induced activation of WNK1 and downstream components. We demonstrate that recombinant NRBP1 can directly induce the activation of WNK4 in vitro. AlphaFold-3 modeling predicts that WNK1, SPAK, NRBP1, and TSC22D4 form a complex, in which two TSC22D4 RΦ-motifs interact with the CCTL1 domain of WNK1 and the CCT domain of NRBP1. Our data indicate that NRBP1 and likely its close homolog NRBP2 function as an upstream activator of the WNK pathway. - Source: PubMed
Publication date: 2025/07/16
Amnekar Ramchandra VDite TobyLis PawelBell SebastianBrown FionaJohnson ClareWilkinson StuartRaggett SamanthaDorward MarkWightman MelMacartney ThomasSoares Renata FLamoliatte FredericAlessi Dario R - The with-no-lysine (K) (WNK) kinases regulate processes such as cell volume and epithelial ion transport through the modulation of cation chloride cotransporters such as the NaCl cotransporter (NCC) present in the distal convoluted tubule (DCT) of the kidney. Recently, the interaction of WNKs with nuclear receptor binding protein 1 (NRBP1) and transforming growth factor-β-stimulated clone 22 domain (TSC22D) proteins was reported. Here, we explored the effect of NRBP1 and TSC22Ds on WNK signaling in vitro and in the DCT. TSC22D1.1, TSC22D2, and NRBP1 are localized in DCT WNK bodies, which are cytoplasmic biomolecular condensates associated with WNK activation. In HEK293 cells, long TSC22D isoforms and NRBP1 increase WNK4 activity. DCT-specific NRBP1-knockout mice have reduced NCC phosphorylation and activate a compensatory response. Thus, NRBP1 and long TSC22D proteins are positive modulators of WNK signaling and modulate Na reabsorption in the kidney. NRBP1 and TSC22Ds likely influence WNK signaling in other tissues, affecting various physiological processes. - Source: PubMed
Publication date: 2025/07/16
Magaña-Ávila GermánCarbajal-Contreras HéctorAmnekar Ramchandra VDite TobyTéllez-Sutterlin MichelleGarcía-Ávila KevinMarquina-Castillo BrendaLopez-Saavedra AlejandroVazquez NormaRojas-Ortega EréndiraDelpire EricEllison David HAlessi Dario RGamba GerardoCastañeda-Bueno María - The With No lysine (WNK) kinases regulate processes such as cell volume and epithelial ion transport through the modulation of Cation Chloride Cotransporters such as the NaCl cotransporter, NCC, present in the distal convoluted tubule (DCT) of the kidney. Recently, the interaction of WNKs with Nuclear Receptor Binding Protein 1 (NRBP1) and Transforming Growth Factor β-Stimulated Clone 22 Domain (TSC22D) proteins was reported. Here we explored the effect of NRBP1 and TSC22Ds on WNK signaling in vitro and in the DCT. TSC22D1.1, TSC22D2, and NRBP1 are localized in DCT WNK bodies, which are cytoplasmic biomolecular condensates associated with WNK activation. In HEK293 cells, long TSC22D isoforms and NRBP1 increase WNK4 activity. DCT-specific NRBP1 knockout mice have reduced NCC phosphorylation and activate a compensatory response. Thus, NRBP1 and long TSC22D proteins are positive modulators of WNK signaling and modulate Na reabsorption in the kidney. NRBP1 and TSC22Ds likely influence WNK signaling in other tissues, impacting various physiological processes. - Source: PubMed
Publication date: 2024/12/19
Magaña-Ávila GermánCarbajal-Contreras HéctorAmnekar RamchandraDite TobyTéllez-Sutterlin MichelleGarcía-Ávila KevinMarquina-Castillo BrendaLopez-Saavedra AlejandroVazquez NormaRojas-Ortega EréndiraDelpire EricEllison David HAlessi Dario RGamba GerardoCastañeda-Bueno María - To investigate the anti-liver cancer effect of 2-hydroxy-3-methyl anthraquinone (HMA) and the specific mechanism based on nicotinamide adenine dinucleotide-dependent protein deacetylase sirtuin-1 (SIRT1)/cellular tumor antigen p53 (p53) pathway. - Source: PubMed
Shuang W UQiao L IXieying ZhuTaoyuan Zhang - Tracking the localization and proximal interaction partners of endogenous proteins provides valuable functional insight. Here, we present a protocol for CRISPR-based endogenous protein tagging in mammalian cells. We describe steps for endogenously tagging human TSC22D2 and MAP4, including designing Cas9 and Cas12a guides for knockin, modularized repair template design and cloning, and procedures for lipid transfection and electroporation. This protocol accommodates Cas nucleases in plasmid expression or ribonucleoprotein complex (RNP) formats. This "endo-tagging" approach offers flexibility and broad applicability. For complete details on the use and execution of this protocol, please refer to Xiao et al.. - Source: PubMed
Publication date: 2024/10/19
Xiao Yu-XiWei JiarunMoffat Jason