Ap3b1 antibody - N-terminal region (ARP33647_P050)
- Known as:
- Ap3b1 (anti-) - N-terminal region (ARP33647_P050)
- Catalog number:
- arp33647_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- Ap3b1 antibody - N-terminal region (ARP33647_P050)
Related genes to: Ap3b1 antibody - N-terminal region (ARP33647_P050)
- Gene:
- AP3B1 NIH gene
- Name:
- adaptor related protein complex 3 subunit beta 1
- Previous symbol:
- -
- Synonyms:
- ADTB3A, HPS2
- Chromosome:
- 5q14.1
- Locus Type:
- gene with protein product
- Date approved:
- 2000-09-01
- Date modifiied:
- 2019-04-23
Related products to: Ap3b1 antibody - N-terminal region (ARP33647_P050)
Related articles to: Ap3b1 antibody - N-terminal region (ARP33647_P050)
- RNA-Sequencing (RNA-Seq) represents a powerful approach for discovering SNPs in coding regions (cSNPs) which can alter the amino acid sequence of the encoded proteins and have predicted deleterious effects in proteins, underlying disease susceptibility or resistance. RNA-Seq data from peripheral blood (PB) and ileocecal valve (ICV) samples collected from fourteen Holstein cattle with focal ( = 5) and diffuse ( = 5) paratuberculosis (PTB)-associated lesions and without lesions ( = 4) in gut tissues was used to identify deleterious cSNPs that were unique to each group of animals. PB and ICV samples from each animal were subjected to RNA extraction, library preparation, and paired-end RNA-Sequencing (RNA-Seq). The RNA-Seq reads were aligned against the bovine ARS-UCD1.2.109 reference genome using the aligner generating an average of 21,331,835 and 19,506,829 uniquely mapped reads in the PB and ICV samples, respectively. SNP calling was performed on the RNA-Seq data of each group of animals using . To ensure high-confidence cSNP calls, highly stringent SNP filtering criteria were applied: minimum read depth (≥ 10), supporting reads for alternative allele (≥ 4), Phred score of the alternative allele (≥ 30), minor allele frequency ( 20%), maximum proportion of missing data per site ( 80%), and distance from indels (SNPs within 5 bp of insertions/deletions were excluded). From the 856, 625, and 603 identified cSNPs that were uniquely present in the transcriptome of the control cows and cows with focal and diffuse lesions, 31, 15, and 31 variants had predicted deleterious effects, respectively. The gene () was the only candidate gene affected by different predicted deleterious cSNPs in the three groups of animals. Using the candidate genes, gene set enrichment analysis (GSEA) revealed distinct biological processes and metabolic pathways associated with each group of cows. Cows without lesions showed enrichment in 11 GO terms and 6 metabolic pathways, particularly involving , , and genes. These leading-edge genes are linked to antigen processing and presentation, phagosome maturation, lysosome function, and intestinal immune homeostasis. Cows with focal lesions had enrichment in the negative regulation of apoptosis and cellular metabolism with two leading-edge genes, and . Predicted deleterious cSNPs in these leading-edge genes may help the host modulate immune responses and maintain low bacterial load during the subclinical stage of MAP infection. Finally, cows with diffuse lesions showed enrichment in 27 metabolic pathways, including Th1/Th2 cell differentiation, antigen presentation, bile secretion, and antifolate resistance. Further validation of the cSNPs and candidate genes in additional independent populations may lead to their use in SNP-based selection strategies for increasing resistance to MAP infection. - Source: PubMed
Publication date: 2026/01/26
Badia-Bringué GerardLam StephanieCánovas ÁngelaAlonso-Hearn Marta - Carcass traits are among the most critical production characteristics in broiler chickens. Therefore, the precise identification of genetic variants and candidate genes affecting these traits is highly valuable for enhancing breeding efficiency and overall productivity in poultry. In this study, a genome-wide association study (GWAS) was conducted to analyse seven carcass traits in 1 498 cocks from a Chinese indigenous yellow-feathered meat chicken population, which had been subjected to three generations of selective breeding for improved BW and feed conversion ratio. The assessing traits included breast muscle weight (BMW), BWs at 42 and 56 days (BW42, BW56), carcass weight (CW), eviscerated weight (EW), eviscerated weight with giblets (EWG), and leg weight (LW). Six significant single-nucleotide polymorphism (SNP) sites were identified by using the "Jingxin No. 1″ 55 K SNP microarray and bioinformatic analysis. These SNPs were annotated to six candidate genes on chromosomes 1, 2, 3, 4, 9, and Z, including SPHKAP, ALCAM, CMTM7, ADAT2, LIMCH1 and AP3B1, with the variant in the SPHKAP gene harboured a frameshift deletion in the coding region. Our study identified novel variants related to chicken carcass traits, which provide a new insight into the genetic mechanism of chicken carcass traits. These trait-associated genetic variants could facilitate the selection of more efficient broilers. - Source: PubMed
Publication date: 2025/12/23
Pan ZGeng HLi DCheng XLiu Y - During evolution, organisms evolve mainly through natural and artificial selection, leaving distinctive signatures on genomic coordinates. Such genomic regions offer valuable insights into the molecular mechanisms that influence quantitative traits. India harbours a diverse buffalo population with Murrah breed exhibiting exceptional milk production and quality, notably a high fat and solids-not-fat content. Therefore, the present investigation focused on exploring selection signatures within the genome of the Murrah buffalo through whole-genome resequencing. A total of 17 472 799 SNPs were identified, which were further utilized for identification of selection signatures using site frequency spectrum-based Tajima's D and Nucleotide Diversity; and linkage disequilibrium-based iHS approaches. A total of 248 regions under selection overlapped with 64 QTLs across various traits (milk, production, reproduction, meat and carcass, health, and exterior) on chromosomes 5, 9, and 17. A majority of the identified QTLs (39) were associated with milk-related traits, with 27 QTLs specifically linked to milk fat content. Notably, genes such as , and mapped within the QTLs under selection are implicated in milk traits, while is associated with growth. Hub genes included 3 (milk); (reproduction); (body confirmation), and (heat tolerance). This study lays the groundwork for targeted breeding efforts aimed at enhancing milk production in buffalo. - Source: PubMed
Surati UtsavNiranjan Saket KPundir Rakesh KumarKoul YmberzalVohra VikasGandham Ravi KumarKumar Amod - A Petit Bleu de Gascogne (PBDG) dog presented with a progressive neurological disorder characterized by hind-limb weakness, anxiety and hallucinatory episodes, lip smacking, progressive vision loss, muscle atrophy, and ataxia. Magnetic resonance imaging revealed diffuse brain atrophy. The dog was euthanized at approximately 23 months of age due to the progression of neurological signs. A study was undertaken to identify the molecular genetic basis of the disorder in this dog. - Source: PubMed
Publication date: 2025/11/11
Then AlexanderWelly RebeccaBullock GarrettChevallier LucieKatz Martin L - NPM1 is a disease-defining gene in the diagnosis of acute myeloid leukemia (AML) and is important for measurable residual disease (MRD) assessment. Over 50 different NPM1 mutations have been described, but only the 3 most common are routinely monitored during follow-up. - Source: PubMed
Wierenga Albertus T JHesp Lucy BSimpelaar ArjanMorsink Linde MWoolthuis Carolien MSchuringa Jan JacobMinovic IsidorHuls GerwinMulder André B