LHX3 antibody - middle region (ARP33644_P050)
- Known as:
- LHX3 (anti-) - middle region (ARP33644_P050)
- Catalog number:
- arp33644_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- LHX3 antibody - middle region (ARP33644_P050)
Related genes to: LHX3 antibody - middle region (ARP33644_P050)
- Gene:
- LHX3 NIH gene
- Name:
- LIM homeobox 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 9q34.3
- Locus Type:
- gene with protein product
- Date approved:
- 2000-03-22
- Date modifiied:
- 2015-09-03
Related products to: LHX3 antibody - middle region (ARP33644_P050)
Related articles to: LHX3 antibody - middle region (ARP33644_P050)
- Post-mitotic neurons undergo a protracted maturation period as they extend axons to their targets, form synapses, and refine electrophysiological properties. We use temporal single-nucleus multiome sequencing to map molecular underpinnings of maturation in mouse skeletal motor neurons (SMNs). Our data show that SMNs undergo transcriptional changes as they mature, but more strikingly, we find that diversity within SMNs changes dramatically as they transition from axon targeting to forming functional circuits. Embryonic SMNs comprise dozens of transcriptional subclusters representing known motor columns and pools, which utilize specific transcriptional programs to innervate unique muscle targets. However, this transcriptional diversity is largely lost by early postnatal life, and SMNs re-diversify into adult alpha, gamma, and type3 identities, which innervate different fibers within each muscle to regulate movement. This work shows that transcriptional subtype diversity within a neuron type, and the underlying regulatory programs, can transform completely during maturation to accommodate changing functional needs. - Source: PubMed
Publication date: 2026/09/30
Chen YijiaChi Hsuan-MingTian AileenStains CourtneyMiller AlisonPatel Tulsi - The pituitary gland is a master regulator of endocrine and reproductive physiology; however, the lack of physiologically relevant in vitro models in livestock species has limited studies of pituitary development and endocrine function. Although pituitary organoids have been generated from human and mouse pluripotent stem cells, comparable models remain unavailable in large animal species. Here, we established functional pituitary-like organoids from ovine embryonic stem cells (oESCs) using a three-dimensional differentiation strategy. The resulting organoids self-organized into Rathke's pouch-like structures and acquired pituitary progenitor characteristics, as demonstrated by the robust expression of LHX3, PITX1, ISLET1 and PROP1. Compared with conventional two-dimensional differentiation, the organoids displayed enhanced structural organization and improved lineage specification. Notably, the organoids exhibited functional gonadotroph maturation, with GnRH treatment significantly upregulating FSHB and LHB expression, as confirmed by immunohistochemistry, qPCR and Western blot. SMART-Seq transcriptome analysis revealed progressive loss of pluripotency-associated signatures and activation of pituitary developmental programs, including sustained expression of lineage-associated regulators such as PAX6. Collectively, this study establishes the first ovine pituitary-like organoid model and demonstrates its capacity to generate functional GnRH-responsive gonadotroph cells. This platform provides a valuable resource for investigating pituitary development, reproductive endocrinology, livestock fertility, and endocrine disease mechanisms in a large-animal context. - Source: PubMed
Publication date: 2026/07/28
Yang HuaXu HuiLv WenliCai YuLi ShanglaiWan YongjieJitjumnong JakreeLuo XiaojuanWang FengZhang Yanli - Inflammatory bowel disease (IBD) and osteoporosis (OP) often co-occur, with IBD accelerating OP onset, though the underlying mechanisms remain unclear. - Source: PubMed
Publication date: 2026/08/12
Su YueLuo XiaohuiXu Haitao - Variants in LHX3, encoding a LIM-homeodomain transcription factor essential for pituitary and neuronal development, are a rare cause of combined pituitary hormone deficiency (CPHD). Affected patients typically exhibit deficiencies of growth hormone (GH), thyrotropin (TSH), prolactin (PRL), and gonadotropins, often accompanied by cervical spine rigidity or sensorineural hearing loss. - Source: PubMed
Publication date: 2026/08/05
Çetin Sirmen KızılcanDikmen İbrahimÖzsu ElifAycan ZehraBerberoğlu MerihŞıklar Zeynep - LHX3, a LIM-homeodomain transcription factor, is crucial in pituitary development. Mutations of LHX3 are infrequently described in the Indian subcontinent. We report two siblings born of consanguineous marriage with combined pituitary hormone deficiency (CPHD) who presented with short stature and extra-pituitary skeletal abnormalities. Whole-exome sequencing (WES) of both siblings revealed an identical novel in-frame deletion variant in the LHX3 gene c.634_636del (p.Glu212del). Both parents of the patients were heterozygous carriers of the same LHX3 variant. The genetic sequencing data did not reveal any other potentially causal variants of other candidate genes that could be associated with CPHD. Furthermore, the conservation of the reference region across species and the expression of LHX3 in pituitary tissue provide additional evidence supporting its pathogenic role. This in-frame deletion, according to theoretical models, could potentially disrupt protein-protein interactions, DNA binding, and protein stability, ultimately disrupting the specification of pituitary cell types. Both patients exhibited a remarkable response to recombinant growth hormone therapy. This novel variant provides a foundation for future functional studies and contributes to the expanding genetic spectrum of LHX3 variants in CPHD. - Source: PubMed
Publication date: 2026/06/29
Palaniappan SreenivasanSridhar SubbiahThasarathan SenthilkumarShanmugam DhivyaDutta Pinaki