CLDN15 antibody - C-terminal region (ARP33635_T100)
- Known as:
- CLDN15 (anti-) - C-terminal region (ARP33635_T100)
- Catalog number:
- arp33635_t100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- CLDN15 antibody - C-terminal region (ARP33635_T100)
Related genes to: CLDN15 antibody - C-terminal region (ARP33635_T100)
- Gene:
- CLDN15 NIH gene
- Name:
- claudin 15
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 7q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-11-26
- Date modifiied:
- 2016-10-05
Related products to: CLDN15 antibody - C-terminal region (ARP33635_T100)
Related articles to: CLDN15 antibody - C-terminal region (ARP33635_T100)
- Tight junctions (TJs) are composed of anastomosing strands between epithelial cells. Members of the claudin family of proteins reside within TJ strands and either seal the paracellular space or assemble into charge and size-selective pathways. Functional studies suggest that claudin-mediated conductance pathways resemble traditional ion channels. However, such postulated pores have not been directly visualized. Using a model claudin deficient epithelium where exogenously introduced EGFP-CLDN15 is the only claudin family member expressed, our investigation sheds light on the arrangement and structure of the postulated claudin pores. Following correlative light and electron microscopical identification of TJs and cryo-electron tomography, we identified series of linearly distributed electron lucent features that locate between two closely apposed plasma membranes of adjacent cells. At these sites, the median spacing between adjacent features is 2.25 nm (IQR = 1.83), with a median 1.66 nm (IQR = 0.92) diameter. In contrast, such features were not observed in claudin deficient model epithelium with exogenous mCherry-ZO-1 expression. These findings agree with the postulated and extensively modeled claudin pores formed within the simple columnar epithelium. This provides the first direct evidence of paracellular pore organization and paves way for future biophysical investigation. - Source: PubMed
Publication date: 2026/02/20
Demchenko Evgeniya AMcGuinness SarahWood ShaunKainov JosephPappas VictoriaAustin JothamHyatt DominicKhalili-Araghi FatemehShen LeWeber Christopher R - Claudin-15 (CLDN15) molecules form channels that directly regulate cation and water transport. In the gastrointestinal tract, this transport indirectly impacts nutrient absorption. However, the mechanisms governing ion transport through these channels remain poorly understood. We addressed this question by building on our previous cell culture studies and an all-atom molecular dynamics simulation model of CLDN15. By mutating D55 to a bulkier glutamic acid or neutral amino acid asparagine, our in vitro measurements showed that the D55E mutation decreased charge selectivity and favored small ion permeability, while the D55N mutation led to reduced charge selectivity without markedly altering size selectivity. By establishing a simplified (reduced) CLDN15 molecular dynamics model that excludes nonessential transmembrane regions, we were able to probe how D55 modified cation dehydration, charge interaction, and permeability. These results provide novel insight into organization of the CLDN15 selectivity filter and suggest that D55 plays a dual role in shaping both electrostatic and steric properties of the pore, but its electrostatic role is more prominent in determining CLDN15 cation permeability. This knowledge can be used toward the development of effective strategies to modulate CLDN15 function. The experimental approach established can be further extended to study the function of other claudin channels. Together, these advancements will help us to modulate tight junctions to promote human health. - Source: PubMed
Publication date: 2026/02/12
McGuinness SarahLi PanLi YeFuladi ShadiKonar SukanyaSajjadi SamanehSidahmed MohammedLi YueyingShen LeKhalili-Araghi FatemehWeber Christopher R - Altered DNA methylation (DNAm) patterns have been proven to play a key role in Crohn's disease (CD) pathogenesis. However, DNAm and its association with disease status in Chinese CD remain unclear. This study systematically examines DNAm patterns in Chinese patients with CD and their association with disease status. By elucidating specific DNAm alterations involved in CD pathogenesis, it aims to provide a molecular foundation for early diagnosis, prognosis assessment, and personalized treatment strategies. In this study, 24 adult treatment-naïve patients with CD were enrolled between January 2022 and May 2023. We performed reduced representation bisulfite sequencing (RRBS) on paired inflamed and non-inflamed intestinal mucosa samples from these patients, and inflammation-specific and disease severity-specific differential methylation signatures were identified. A total of 17,097 differentially methylated sites (DMCs) and 2,687 differentially methylated regions (DMRs) were identified in inflamed mucosa. Biological association analysis revealed that inflammation-associated DMRs were enriched in immune function, with 123 DMRs annotating 89 genes involved in immune cell function while 173 DMRs annotating 117 genes participated in cell adhesion function. Analysis of DNAm profiles of inflamed mucosal samples by disease severity revealed that 389 DMRs were associated with the Simple Endoscopic Score for Crohn's Disease (SES-CD) and 327 DMRs with the Crohn Disease Activity Index (CDAI). Of these, six genes, KDM4B, CLDN15, PGGHG, SLC25A10, KIAA2013, and N4BP1, were significantly associated with inflammation, SES-CD and CDAI. Hence, DNAm reflects immunological changes in the gut of CD patients and discriminates patients based on disease severity, highlighting its potential as a predictive marker for disease management. - Source: PubMed
Publication date: 2026/01/22
Zhang TianyuLin QiaowenXu Wang-YangHe QiyeGong ChengxiangWang LeiWang ZhengtingZhong Jie - Breast Cancer (BrCa) remains a devastating disease presenting emerging needs for effective management. Recently, epigenetic biomarkers are assessed in liquid biopsy for diagnostic and prognostic applications. This study applies a 3-step data-driven biomarker discovery pipeline to identify robust methylation biomarkers and generate high-performance biosignatures specific for clinically significant BrCa end-points, followed by laboratory validation in patient cell-free DNA (cfDNA). - Source: PubMed
Publication date: 2026/01/05
Panagopoulou MariaPapadaki Maria AKaraglani MakrinaTheodosiou TheodosisMichaelidou KleitaBaritaki StavroulaTsamardinos IoannisKakolyris StylianosAgelaki SofiaChatzaki Ekaterini - Drought and water scarcity, exacerbated by global warming, are enormous threats to global food sustainability and security. Poultry, in particular, are highly impacted by adverse environmental stressors. As nutrient absorption and intestinal integrity are critical for growth and performance, understanding the impact on the broiler gastrointestinal tract is highly relevant. Here, we examined the effect of chronic cyclic heat stress (HS) on the jejunal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the 4th generation of broiler lines divergently selected for low (LWE)- and high-water efficiency (HWE). Male HWE and LWE broilers (n = 240/line) were allotted to 12 environmental chambers (2 floor pens/chamber, 6 chambers/line, 20 birds/pen) and were exposed to cyclic HS (36°C for 9h/day from 9:00 am to 6:00 pm) or thermoneutral conditions (25°C) from day 29 to 49 of age in a 2 × 2 factorial design. Growth performance and mortality were recorded. At day 49, jejunal tissues were collected for molecular analyses using real-time quantitative PCR and immunoblot. Jejunal gene expression of multiple gut integrity factors were higher (P < 0.05) in the HWE as compared to the LWE lines, including claudin 22 (CLDN22), -34, occluding (OCDN), zona-occludin-2 (ZO-2), gap junction alpha1 (GJA1), GJA3, GJC1, and cadherin 1 (CDH1). CLDN8, -20, -25, -4, GJC2, and GJD2 were also greater (P < 0.05) in HWE, but were additionally downregulated (P < 0.05) during HS. Conversely PALS1-associated tight junction protein (PATJ) and desmocollin 1 (DSC1) mRNAs were significantly downregulated in the HWE as compared to the LWE broilers. Significant interactions between the line and environment were seen in CLDN1, where the expression was decreased in the LWE but increased in the HWE in HS. Additionally, CLDN15 and -16 genes were greatest in the HWE under TN conditions, while catenin alpha 2 (CTNNA2) was highest in the HWE during HS. Overall, the jejunal expression profile of key genes associated with intestinal barrier integrity likely contributes to the water efficiency phenotype and the response of these birds to HS. - Source: PubMed
Publication date: 2025/12/16
Greene Elizabeth SOrlowski SaraDridi Sami