ZNF235 Antibody - N-terminal region (ARP33537_P050)
- Known as:
- ZNF235 Antibody - N-terminal region (ARP33537_P050)
- Catalog number:
- arp33537_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- ZNF235 Antibody - N-terminal region (ARP33537_P050)
Related genes to: ZNF235 Antibody - N-terminal region (ARP33537_P050)
- Gene:
- ZNF235 NIH gene
- Name:
- zinc finger protein 235
- Previous symbol:
- ZNF270, ZFP93
- Synonyms:
- HZF6, ANF270
- Chromosome:
- 19q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-07-19
- Date modifiied:
- 2015-08-27
Related products to: ZNF235 Antibody - N-terminal region (ARP33537_P050)
Related articles to: ZNF235 Antibody - N-terminal region (ARP33537_P050)
- The tumor budding (TB) and fibroblast activation protein (FAP)-positive cancer-associated fibroblasts (CAFs) are associated with patient prognosis in triple-negative breast cancer (TNBC). However, the impact between TB and FAP-positive CAFs on TNBC progression remains poorly understood. This study seeks to investigate the transcriptomic profile of FAP-positive CAFs in high TB TNBC to identify CAFs-derived factors contributing to disease aggressiveness. The 170 formalin-fixed paraffin-embedded TNBC tissues were assessed for TB by pan-cytokeratin immunohistochemistry (IHC). Its clinicopathological correlations were examined through univariate and multivariate analyses, with overall survival (OS) evaluated using Kaplan-Meier analysis. CAFs whole transcriptomic profiles of 13 TNBCs was conducted using Templated Oligo-Sequencing and the differential expression was analyzed in R studio with the DESeq2 package. Pathways associated with differentially expressed genes were identified using the Enrichr package. The level of leukemia inhibitory factor (LIF) was explored by IHC in 141 TNBCs, and its prognostic significance was determined. The induction of TNBC cell migration and expression of programmed death-ligand 1 (PD-L1) by a recombinant LIF, along with attenuation by EC359, a LIF inhibitor, were investigated using Transwell assay and flow cytometry. High TB was observed in 46.5% of TNBC patients and significantly associated with shorter OS, in which the upregulated MAPK cascade, ERK1/ERK2 signaling, and protein kinase B regulation signaling pathways were involved. In FAP-positive CAFs, pathways related to tyrosine phosphorylation, STAT protein tyrosine phosphorylation, and G-protein-coupled receptor regulation were also elevated. The overexpressed genes in FAP-positive CAFs from high TB-TNBCs included ZNF235, ANKRD30B, SLC26A2, SPDYC, CDKN1C, LIF, and FAM83A. The secreted LIF was found in 41.1% of TNBC cases with high levels detected in CAFs, while 26.2% of cases showed high LIF production in cancer cells. Recombinant LIF significantly promoted breast cancer cell migration and enhanced PD-L1 expression. These effects were attenuated by EC359. This study highlights the correlation of high TB in TNBC with poor prognosis and upregulation of key signaling pathways in CAFs. FAP-positive CAFs overexpress genes like LIF, which promotes cell migration and PD-L1 expression. LIF inhibition reduces these effects, suggesting LIF as a potential therapeutic target in TB-associated TNBC progression and immunotherapy. - Source: PubMed
Publication date: 2025/11/25
Phankeaw PimchanokKhanaruksombat SuparadaNumprasit WarapanJamjuntra PranisaAugsornworawat PunnWarnnissorn MaleeThuwajit PetiThuwajit Chanitra - Amyotrophic lateral sclerosis (ALS) is an age-associated, fatal neurodegenerative disorder causing progressive paralysis and respiratory failure. The genetic architecture of ALS is still largely unknown. - Source: PubMed
Publication date: 2024/09/06
Li SitingGui JiangPassarelli Michael NAndrew Angeline SSullivan Kathleen MCornell Kevin ATraynor Bryan JStark AliChia RuthKuenzler Rebecca MPioro Erik PBradley Walter GStommel Elijah W - Genetic and physical mapping studies indicate that hundreds of zinc-finger (ZNF)-containing genes populate the human genome and that many of these genes are arranged in familial clusters. However, the extent to which these tandemly arrayed families are conserved among mammalian species is largely unknown. In a previous study, we identified a conserved cluster of Kruppel-associated box (KRAB)-containing ZNF genes located near the XRCC1 gene in human chromosome 19q13.2 and mouse chromosome 7 and analyzed two members of the murine gene family, Zfp93 and Zfp94, in detail. Here we report the identification and characterization of putative human orthologs of these murine genes. The human genes ZFP93 and ZNF45 are substantially similar to their murine counterparts in overall structure, but two notable differences exist between the sets of genes. First, the human genes encode more ZNF repeats than their murine counterparts. Second, the ZNF repeats that are common to orthologs exhibit varying degrees of conservation. Expression studies indicate that the human genes, like their mouse equivalents, are expressed widely and are coexpressed at similar levels in most adult tissues. These comparative gene sequence and expression studies therefore suggest that at least two members of the mammalian XRCC1-linked KRAB-ZNF gene family were elaborated prior to the divergence of primate and rodent lineages and were well conserved in human and mouse. - Source: PubMed
Shannon MStubbs L