Foxl2 antibody - middle region (ARP33358_P050)
- Known as:
- Foxl2 (anti-) - middle region (ARP33358_P050)
- Catalog number:
- arp33358_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- Foxl2 antibody - middle region (ARP33358_P050)
Related genes to: Foxl2 antibody - middle region (ARP33358_P050)
- Gene:
- FOXL2 NIH gene
- Name:
- forkhead box L2
- Previous symbol:
- BPES
- Synonyms:
- BPES1
- Chromosome:
- 3q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-02-28
- Date modifiied:
- 2019-04-23
Related products to: Foxl2 antibody - middle region (ARP33358_P050)
Related articles to: Foxl2 antibody - middle region (ARP33358_P050)
- Testicular adult granulosa cell tumors (AGCTs) are rare and show several clinical, pathological, and molecular differences with their ovarian counterparts. FOXL2 p.Cys134Trp, the ubiquitous molecular driver of ovarian AGCTs, is infrequent (~ 7%) in testicular AGCTs. Recently, FGFR1 hotspot mutations were reported as a potentially "alternative molecular driver" in FOXL2-wild type (WT) ovarian AGCTs. A systematic assessment of FGFR1 status has not been performed in testicular AGCTs. Recently, our group analyzed a series of twenty testicular AGCTs using two NGS panels that lacked coverage of FGFR1. Among twelve cases analyzed successfully, none harbored pathogenic FOXL2 variants. In this study, we reassessed the tumors from our prior series with an NGS panel that covers FGFR1. Among the 14 tumors (70%) that were sequenced successfully, none harbored pathogenic FGFR1 variants. Considering the AGCTs assessed in this study and those previously reported in the literature, none of the 24 tumors analyzed to date have shown pathogenic FGFR1 variants. The present study reinforces the concept that testicular sex cord-stromal tumors classified as AGCTs are different from ovarian counterparts. - Source: PubMed
Publication date: 2026/04/18
Ricci Costantinode Biase DarioMaloberti ThaisOrsatti AgneseUlbright Thomas MIdrees Muhammad TOliva EstherCornejo KristineLobo JoãoMichalova KvetoslavaRaspollini Maria RosariaWilliamson Sean Rvan Leenders Geert J L HKao Chia-SuiMaclean FionaSangoi Ankur ROsunkoya Adeboye OFiorentino MichelangeloDe Leo AntonioTallini GiovanniAcosta Andres Martin - The ovarian granulosa cells are responsible for producing hormones and supporting oocytes through maturation and meiotic resumption. There is a need to generate granulosa-like cells (GLCs) from human induced pluripotent stem cells (hiPSCs) to better model human gonadal development and to test the effects of exogenous or pharmaceutical compounds on the ovary. Here we report a rapid ligand-based protocol for differentiating hiPSCs into cells that express markers of the transient developmental lineages and steroidogenic pathway genes. Single-cell RNA-sequencing (scRNA-seq) analysis identified canonical granulosa cell genes were expressed in a subset of cells and identified new genes of interest that were significantly associated with computationally modeled pseudotime. HSD17B1 was expressed in resulting GLCs but at low levels, suggesting an immature granulosa cell phenotype. The GLCs were produced using a simple culture method that could be augmented for granulosa cell functions such as sustaining oocyte growth. Producing GLCs through protocols such as this one is a first step toward designing large-scale ovarian endocrinology assays and developing personalized cell-based fertility and hormone restoration technologies in the future. This rapid protocol produced cells that express steroidogenic enzyme genes etoc blurb. Kubo and colleagues present a 5-day rapid protocol to generate immature granulosa-like cells from hiPSCs. Cells differentiated with inhibition of DKK1, a WNT signaling target gene, expressed gonadal ridge markers and FOXL2 transcripts and protein. Additionally, steroidogenic enzyme genes were expressed. A small population of differentiated cells were identified as expressing early-stage granulosa cell genes by single-cell RNA-seq. - Source: PubMed
Publication date: 2026/04/01
Kubo HanaLu LinaVanZanten MayaThyagraj JanaviSaunders Diane CGao RuliLaronda Monica M - Pacific oysters (Crassostrea gigas) are a major aquaculture species among molluscs worldwide, possessing significant ecological and economic value. The mechanism governing sex determination in oysters remains largely unknown. Fox transcription factors are key regulators of vital biological processes, controlling important functions such as tissue development and reproduction. This study presents a genome-wide identification and characterization of the Fox gene family in C. gigas. Through systematic identification, 16 Fox genes were discovered and classified into 13 subfamilies based on phylogenetic analysis and conservation domain analysis. Transcriptomic profiling revealed that FoxK2 and FoxG1 were significantly upregulated during the resting stage, whereas FoxG1 expression was higher in females and males throughout the active gametogenesis stage (P < 0.05). FoxE1 and FoxL2 exhibited elevated expression profiles during female maturation, whereas FoxO, FoxN4, and FoxK2 showed higher expression profiles during male maturation (P < 0.05). At the spawning stage, FoxE1 and FoxK2 were significantly expressed in females, whereas FoxJ1B and FoxK2 exhibited elevated expression in males (P < 0.05). Our findings indicate sexually dimorphic expression patterns of FoxB1, FoxE1, FoxL2, FoxP1, and FoxC1 and their gonad-specific functions. Our research has significant ecological implications for comprehending the sex determination process and offers fresh insights into the Fox gene regulation mechanisms in bivalves. - Source: PubMed
Publication date: 2026/03/22
Chen SitongLi QiHu BiyangDu Shaojun - The freshwater snail is both ecologically and economically significant, exhibiting notable sexual dimorphism in growth and nutritional traits that underscore the importance of breeding of monosex stocks. However, the genetic basis of sex determination remains unclear. Herein, genome-wide association studies (GWASs) combined with transcriptomic analysis were conducted to identify sex-linked markers and candidate genes for this species. GWAS generated 571 significantly sex-associated SNPs and 1853 InDels, corresponding to 44 candidate genes. Multiple significant SNP peaks were detected on chromosomes 1 and 2, with and as key candidate genes. A sex-linked InDel marker located within can distinguish males and females cost-effectively. Genotype analysis of the sex-associated loci revealed that most females were homozygous while males were heterozygous, suggesting that has a primarily XX/XY sex determination system. Comparative gonadal transcriptome analyses identified 2996 female-biased and 4281 male-biased genes. Among them, , , and may be critical in male sex differentiation, while , , and may be important in female sex differentiation. Integration of GWAS and transcriptomic data highlighted four pronounced sex-associated candidate genes, including , , and . These results provide a valuable foundation for elucidating the genetic mechanisms underlying sex determination and for the development of monosex stocks in . - Source: PubMed
Publication date: 2026/03/14
Gao YajunWen YanhongYi ShaokuiLin YongPeng JinxiaPan XianhuiZhou Xiaoyun - Sex determination (SD) in zebra and quagga mussels, two globally invasive freshwater bivalves responsible for major ecological and economic impacts, has remained unexplored. Using chromosome-scale genomes and whole-genome resequencing of 80 individuals, we uncover strikingly different SD architectures: zebra mussels exhibit a polygenic ZZ ZW system, with FoxL2 among the prominent female-associated candidates. In contrast, quagga mussels carry a highly localized ~800 kb XX XY SD region containing FoxL2-Y, a novel candidate male-determining locus that arose through duplication and divergence of FoxL2. K-mer analyses and staged embryonic transcriptomes reveal rare but specific FoxL2-Y expression consistent with an early regulatory role in male determination. The quagga mussel SD region is also enriched for C-lectins, a gene family implicated in gamete-interaction processes, supporting a model in which haploid selection facilitated SD turnover. These findings reveal unexpected SD divergence between closely related species and illuminate mechanisms enabling rapid evolution of reproductive systems in invasive taxa. - Source: PubMed
Publication date: 2026/03/20
Weber Alexandra A-TUthanumallian KavithaKocot Kevin MGiulio MarcoSignorini Silvia GSenut Marie-ClaudeChen ZeyuanSigwart Julia DPassamaneck Yale