SHOX2 antibody - middle region (ARP33286_T100)
- Known as:
- SHOX2 (anti-) - middle region (ARP33286_T100)
- Catalog number:
- arp33286_t100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- SHOX2 antibody - middle region (ARP33286_T100)
Related genes to: SHOX2 antibody - middle region (ARP33286_T100)
- Gene:
- SHOX2 NIH gene
- Name:
- short stature homeobox 2
- Previous symbol:
- -
- Synonyms:
- SHOT, OG12X, OG12
- Chromosome:
- 3q25.32
- Locus Type:
- gene with protein product
- Date approved:
- 1998-06-05
- Date modifiied:
- 2016-04-25
Related products to: SHOX2 antibody - middle region (ARP33286_T100)
Related articles to: SHOX2 antibody - middle region (ARP33286_T100)
- Lung adenocarcinoma (LUAD) presents a paradoxical association between SHOX2 DNA hypermethylation-a well-validated diagnostic biomarker-and its transcriptional overexpression, challenging conventional methylation-cancer biology paradigms. Through validation in our clinical cohort (n = 142) and functional experiments, we demonstrate that SHOX2 overexpression is linked to oncogenic activation, correlates with advanced TNM stage (P = 0.0161), poor tumor differentiation (P = 0.0418), and reduced overall survival (HR = 1.583, 95% CI:1.027-2.442), establishing its role as an independent prognostic factor (multivariate Cox HR = 1.704, P = 0.021). Mechanistically, systematic literature/patent analyses revealed that commercially utilized SHOX2 hypermethylation biomarkers (e.g. Epi proLung BL, LungMe® kits) target CpG sites within the first intron-a gene body region whose methylation enhances oncogenic transcription-rather than promoter regions. Integrative TCGA-LUAD methylation profiling and demethylation experiments (5-aza-dC treatment in H1650/SK-LU-1/H1975/A549/H1299/H322/HCC827/H358 cells) further establish a dual regulatory mechanism: promoter hypomethylation (cg25694447/cg26129769 sites, P < 0.0001) and gene body hypermethylation (cg09220088/cg04521004 sites, P < 0.0001) cooperatively drive SHOX2 overexpression. This spatial resolution explains the high specificity of SHOX2 methylation-based diagnostics despite tumor overexpression, resolving longstanding contradictions. Our findings redefine epigenetic regulation in LUAD, demonstrating that regional methylation patterns-not global promoter status-orchestrate oncogene activation. We propose a novel framework for spatially resolved methylomics, advocating 1) dual-target assays monitoring both promoter and gene body methylation to improve diagnostic precision, and 2) therapeutic exploitation of SHOX2's intronic methylome as a druggable epigenetic switch. - Source: PubMed
Publication date: 2026/04/02
Zhou YangZang RuochuanLi ZhaoZhang ZhenWang JuhongZhou XiaoxiangXie FucunChen GuangXue QiHe Jie - DNA methylation serves as a crucial biomarker for early cancer detection, holding great promise in liquid biopsy. However, its low abundance in plasma and interference from complex matrices pose significant challenges to clinical detection sensitivity. To this end, this study developed a novel direct enrichment platform based on methyl-binding domain (MBD) protein-functionalized magnetic nanoparticles (MBD@MNPs). Through systematic optimization, the methylated DNA (mDNA) capture efficiency of MBD@MNPs reached 86.6% with an elution efficiency of 44.9% in simulated systems, while bypassing the cumbersome step of total cell-free DNA (cfDNA) extraction and overcoming plasma matrix interference. In comparison, this strategy enhanced the sensitivity of methylation-specific PCR by 25-fold, with a detection limit as low as 0.04 ng/mL. Applied to plasma samples from patients with various tumors (including colorectal, lung, breast, cervical, liver, and gastric cancers), the technology significantly improved the detection rates up to 100% of tumor-specific methylation biomarkers, such as , SHOX2, RASSF1A, ZNF671, Septin9, and BMP3. Therefore, this study provides an efficient, universal and user-friendly enrichment and accurate detection of mDNA, laying a methodological foundation for early cancer screening and prognosis assessment. - Source: PubMed
Publication date: 2026/04/01
Li YongChen XiangyuWang YeZhao XiaowenDu JiansenShi ChaoMa Cuiping - Lung cancer is a common malignant tumor with high incidence and mortality. Bronchoscopy is key for its diagnosis, but traditional morphology-based pathology has limitations like missed diagnoses. This study explored the auxiliary diagnostic value of gene methylation detection combined with morphology-based pathology in bronchoscopic samples. - Source: PubMed
Publication date: 2026/03/16
Niu XiaojuanHu DixiaLi XiaWang LishaWang Xunbo - DNA methylation profiling has emerged as a powerful adjunct for refining pathological accuracy. This study investigates the correlation between the SHOX2 and RASSF1A methylation status in intraoperative pleural lavage fluid (IPLF) and indices of tumor aggressiveness in stage I lung adenocarcinoma. - Source: PubMed
Publication date: 2026/03/03
Zhang YufeiChen LiangDai WenjunZhou YahuiShi JianxinXu YeZhou Wenyong - PurposeThis study aimed to investigate KRAB-associated protein 1 () expression in pleural mesothelioma (PM) and its impact on the biological behavior of the human pleural mesothelioma cell line MSTO-211H, providing a specific biomarker for the early clinical diagnosis of PM.Patients and methods expression levels in PM tissues were detected using immunohistochemistry. Lentivirus infection was used to construct MSTO-211H mesothelioma cell lines with stable overexpression or knockdown, and the efficiency of overexpression or knockdown was detected using qRT-PCR(quantitative Reverse Transcription PCR) and Western blotting. The effects of overexpression and knockdown on MSTO-211H mesothelioma cell proliferation, migration, and invasion were detected using cell counting kit-8, plate colony formation, cell scratch, and transwell invasion assays, respectively. The effects of overexpression and knockdown on the cell cycle, related cyclins, and apoptosis were detected using flow cytometry. Gene enrichment and correlation analysis of mesothelioma were performed using bioinformatics analysis.Results was significantly overexpressed in PM tissues compared with normal pleural tissues ( < 0.05). Compared to the control group, overexpression in mesothelioma MSTO-211H cells significantly enhanced proliferation, migration, and invasion ( < 0.05), without causing cell cycle arrest, and significantly increased the mRNA and protein levels of cyclin D1 and cyclin E ( < 0.05), whereas the apoptosis rate did not significantly change ( > 0.05). Conversely, knockdown in mesothelioma MSTO-211H cells significantly inhibited their proliferation, migration, and invasion abilities ( < 0.05), induced G0/G1 phase arrest in the cell cycle, and significantly increased the apoptosis rate ( < 0.05). Spearman correlation analysis revealed significant positive associations between mRNA expression and and . Conversely, expression was significantly negatively correlated with and . GSEA reveals -associated enrichment of DNA repair, cell cycle, and proteostasis pathways in TCGA-MESO.Conclusion is highly expressed in PM and functions as an oncogene-like regulator, enhancing tumor cell growth and aggressiveness.Clinical trial registration2023-28. - Source: PubMed
Publication date: 2026/02/19
Mei WenWang YiqiYang ShengjieFu QunshanXiong WeiZhang Yepin