HDAC2 antibody - C-terminal region (ARP32618_P050)
- Known as:
- HDAC2 (anti-) - C-terminal region (ARP32618_P050)
- Catalog number:
- arp32618_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- HDAC2 antibody - C-terminal region (ARP32618_P050)
Related genes to: HDAC2 antibody - C-terminal region (ARP32618_P050)
- Gene:
- HDAC2 NIH gene
- Name:
- histone deacetylase 2
- Previous symbol:
- -
- Synonyms:
- RPD3, YAF1, KDAC2
- Chromosome:
- 6q21
- Locus Type:
- gene with protein product
- Date approved:
- 1996-11-15
- Date modifiied:
- 2019-02-19
Related products to: HDAC2 antibody - C-terminal region (ARP32618_P050)
Related articles to: HDAC2 antibody - C-terminal region (ARP32618_P050)
- The absence of the protein dystrophin in Duchenne muscular dystrophy (DMD) leads to progressive muscle weakness, failing regeneration and deregulation of nitric oxide (NO) signalling. We focused on L-citrulline, a precursor of L-arginine, required for NO production in muscle, which is reduced in dystrophic mdx muscle. - Source: PubMed
Publication date: 2026/04/22
Tulimiero LisamauraBoccanegra BrigidaMantuano PaolaMele AntoniettaDe Bellis MichelaLenti RobertaSanarica FrancescaCirmi SantaConte ElenaCappellari OrnellaSherrard Amber EGreen ArdawnaSrinivasa MiraFiorotto Marta LDe Luca Annamaria - Loss of Dot1l in Aquaporin 2 progenitor cell (AP)-derived distal renal segments in Dot1lAqp2cre (Dot1l) promotes kidney fibrosis by upregulating endothelin 1 (ET1) through histone deacetylase 2 (HDAC2), defining a Dot1l-HDAC2-ET1 profibrotic pathway. Additionally, Lcn2 was also upregulated in Dot1l mice. Here, we investigated whether and how Lipocalin 2 (Lcn2) contributes to this pathway. We performed in vivo studies using multiple mouse models with targeted deletion of Dot1l, Lcn2, or Edn1 in distal nephron segments or globally. Human CKD RNA-seq data analysis and in vitro studies in IMCD3 cells were conducted to validate the in vivo results. Because Lcn2 mice are available and Lcn2 is barely detectable in IMCD3 cells, Lcn2 down- and upregulation experiments were performed in mice and IMCD3 cells, respectively. Several approaches, including immunofluorescence staining and in situ hybridization, were employed to identify and confirm the Lcn2 profibrotic role. Both Dot1l deletion in AP in Dot1l mice and Dot1l silencing in IMCD3 cells upregulated Lcn2. Global deletion of Lcn2 in Dot1l mice attenuated HDAC2 and ET1 levels and reduced kidney fibrosis, establishing Lcn2 as a downstream effector of Dot1l-deletion-induced kidney fibrosis. Although conditional knockout of Edn1 in Dot1l mice showed Lcn2 expression comparable to that in Dot1l mice, they exhibited reduced kidney fibrosis, suggesting that Lcn2 lies upstream of ET1-mediated injury. Reanalysis of a human chronic kidney disease (CKD) dataset revealed that downregulation of DOT1L was coupled with upregulation of LCN2, HDAC2, and ET1, recapitulating our findings in Dot1l mice. These results were further validated in IMCD3 cells, in which Lcn2 was upregulated by Dot1l silencing, and addition of 293 T cells-secreted or recombinant Lcn2 led to increased HDAC2 and ET1 expression. Loss of Dot1l in AP upregulates Lcn2, which promotes kidney fibrosis by increasing HDAC2 and ET1. Our findings add Lcn2 as a new component to define a more complicated Dot1l-Lcn2-HDAC2-ET1 pro-fibrotic pathway that links AP-derived distal renal segments to kidney fibrosis. - Source: PubMed
Tsilosani AkakiGao ChaoMulroy EvelynPirzada NoorShehzad SanaDas ShreyaSun WeiChen EnuoGuo SunnyKnowles NicoleSajjad FizzaZhang Wenzheng - Kratom (Mitragyna speciosa) is a traditional Southeast Asian botanical long used for alleviating pain and boosting energy. Its chief bioactive compound, mitragynine (MG), exhibits both opioid-like and stimulant properties and has prompted interest in its potential role in pain management and opioid withdrawal support. However, its safety profile and underlying mechanisms remain incompletely understood. This systematic review critically synthesizes preclinical evidence on kratom's molecular, pharmacological, and epigenetic effects. Guided by PRISMA 2020 criteria, studies indexed in Scopus and Web of Science (2000-2024) were analyzed, focusing on receptor activity, intracellular signaling, and gene regulation in in vitro and in vivo models. Among 20 eligible studies, key findings indicate that kratom alkaloids engage μ-opioid, adrenergic, and serotonergic receptors; modulate dopaminergic and glutamatergic systems; and exert anti-inflammatory and analgesic effects. Under chronic exposure followed by withdrawal, MG was associated with reduced histone acetylation and increased HDAC2 expression, while Rab35 emerged as a potential withdrawal-associated biomarker. MG also inhibited cardiac ion channels and altered CYP450 enzyme expression, highlighting safety concerns related to cardiotoxicity and drug-drug interactions. Despite these mechanistic insights, limitations in pharmacokinetic data, standardized dosing, and long-term safety preclude clinical application. Future research should prioritize controlled human studies, omics-driven biomarker discovery, and evidence-based regulatory evaluation to clarify kratom's therapeutic potential and risk profile. - Source: PubMed
Publication date: 2026/04/16
Misnan EdyhamHasbullah Nur Zahidah AqilahAbd Rashid RusdiMohd Shah AishahSim Maw Shin - Human cerebral organoids derived from induced pluripotent stem cells can recapture early developmental processes and reveal changes involving neurodevelopmental disorders. Mutations in the X-linked methyl-CpG binding protein 2 (MECP2) gene are associated with Rett syndrome, and disease severity varies depending on the location and type of mutation. Here, we focused on neuronal activity in Rett syndrome patient-derived organoids, analyzing two types of MECP2 mutations-a missense mutation (R306C) and a truncating mutation (V247X)-using calcium imaging with three-photon microscopy. Compared to isogenic controls, we found abnormal neuronal activity in Rett organoids and altered network function based on graph theoretic analyses, with V247X mutations impacting functional responses and connectivity more severely than R306C mutations. These changes paralleled EEG data obtained from patients with comparable mutations. Labeling >/>DLX promoter-driven inhibitory neurons demonstrated differences in activity and functional connectivity of inhibitory and excitatory neurons in the two types of mutations. Transcriptomic analyses revealed HDAC2-associated impairment in R306C organoids and decreased GABA receptor expression in excitatory neurons in V247X organoids. These findings demonstrate mutation-specific mechanisms of vulnerability in Rett syndrome and suggest targeted strategies for their treatment. - Source: PubMed
Publication date: 2026/04/14
Osaki TatsuyaDelepine ChloeOsako YumaKranz DevorahLevin AprilNelson CharlesFagiolini MichelaSur Mriganka - Seasonal breeders rely on light as a key environmental cue to trigger reproductive activities. While studies have been focused on male white-rumped munia (), the effects on females are largely unknown. This study examined how light compositions affect female reproductive physiology. In the first experiment, female birds were exposed to short day (SD; 8 L:16D) and long day (LD; 15 L:9D) for a single day to study various transcripts involved in seasonal reproduction. The second experiment included SD and LD along with an added photoperiod of 12 L:12D, and the treatment extended for 30 d. In the third experiment, female birds were exposed to the above three photoperiod conditions, including natural day length (NDL), to assess morphological changes across 18 months. The fourth experiment evaluated seasonal variations in reproductive parameters across four months: March, June, September, and December. The fifth focused on illuminance levels (10, 50, and 100 lux) under a photoperiod of 12 L:12D, and the sixth assessed responses to red (650 nm) and blue light (450 nm) at a constant irradiance of 0.5 W/m. In all six experiments, an assessment of body mass and follicular diameter was done, while Experiment 3 specifically examined full-body moult and primary flight feather moult as well. Hypothalamic tissue was analysed for reproductive and epigenetic markers. The first experiment revealed enhanced expression of and in the long-day group, while no significant changes in other reproductive ( and ) and epigenetic ( and ) transcripts were observed. The second experiment observed increased expression of exclusively under 15 L:9D, while and transcripts were increased under 12 L:12D. Experiment 3 demonstrated distinct morphological responses to different photoperiods, with significant increases in follicular diameter under 15 L:9D and NDL conditions at the 7 month, but this effect was delayed until the 10 month under 12 L:12D. In contrast, no change was observed under 8 L:16D. Seasonal variations in transcripts were noted, with the highest expression of , and in September compared to other months. The fifth experiment showed that exposure to 100 lux increased key reproductive markers (, and ) and epigenetic regulators ( and ), while the sixth experiment found no significant differences between red and blue light treatment. Overall, the findings highlight the importance of light duration and illuminance in reproductive responses. While no robust change in epigenetic transcript was observed under laboratory conditions in female birds, the epigenetic changes observed in the seasonal experiment suggest that seasonal reproductive cycles are under regulation of epigenetic mechanisms at the molecular level. - Source: PubMed
Publication date: 2026/04/14
Lalpekhlui RuthHnamler LalsiamkimaTrivedi Amit Kumar