NR5A2 antibody - middle region (ARP32524_P050)
- Known as:
- NR5A2 (anti-) - middle region (ARP32524_P050)
- Catalog number:
- arp32524_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- NR5A2 antibody - middle region (ARP32524_P050)
Related genes to: NR5A2 antibody - middle region (ARP32524_P050)
- Gene:
- NR5A2 NIH gene
- Name:
- nuclear receptor subfamily 5 group A member 2
- Previous symbol:
- FTF
- Synonyms:
- FTZ-F1beta, hB1F, LRH-1, FTZ-F1, hB1F-2, B1F2, LRH1
- Chromosome:
- 1q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 1998-05-13
- Date modifiied:
- 2018-02-14
Related products to: NR5A2 antibody - middle region (ARP32524_P050)
Related articles to: NR5A2 antibody - middle region (ARP32524_P050)
- The androgen receptor (AR) signaling axis is regarded as the key driver of prostate cancer (PCa). Besides acting as a well-characterized transactivator of diverse targets, accumulating evidence suggests that AR can also function as a transrepressor. However, AR-repressed targets and their significance in PCa and castration-resistant PCa (CRPC) remain poorly understood. Among multiple mechanisms, intratumoral androgen biosynthesis is regarded as an important factor responsible for persistent AR signaling in CRPC. Previously, we characterized that the nuclear receptor LRH-1 () plays a key role in the promotion of intratumoral androgen biosynthesis in CRPC via its direct transcriptional control of multiple key steroidogenic enzymes. However, the transcriptional control of LRH-1 in PCa is still undefined. In this study, we show that androgen-activated AR could suppress, whereas antiandrogen-suppressed AR could up-regulate the LRH-1 expression in PCa cells. Furthermore, our genomics analysis showed that the transcriptional repression of by ligand-activated AR was mediated through the induction of a distinct androgen-dependent chromatin looping formed within the topologically associated domain of via direct binding of AR to the regulatory elements of . Our present study demonstrates the significance of decreased androgen levels in androgen-deprivation therapy, resulting in the relief or up-regulation of LRH-1 toward intratumoral androgen biosynthesis in CRPC. - Source: PubMed
Publication date: 2025/10/24
You WenxingGao TiantianLi HaolongLam Daniel Hau TakXie WenjuanXiao LijiaGao WeijieWu DinglanWang JunjianWang YuliangChan Franky Leung - Development of the vertebrate lower jaw depends on spatially precise cell fate decisions by cranial neural crest-derived cells (CNCCs) of the mandibular arch. From closer to the mouth (oral) to farther away (aboral), CNCCs adopt bone, cartilage, tendon, and stromal fates to shape the jaw skeleton and ensure proper connectivity to the musculature. How signaling pathways impact downstream transcriptional programs to generate distinct cell fates along the oral-aboral axis remains incompletely understood. Using photoconversion-based lineage tracing of CNCCs in zebrafish, we show that oral cells contribute to the lower jaw skeleton and aboral cells primarily to tendon, ligament, and stromal tissues. During embryogenesis, the oral domain is partitioned into lateral + and medial + subdomains distinct from an aboral +, + domain. Using pharmacological inhibition and transgenic misexpression, we find that Bmp signaling establishes aboral and expression, Fgf signaling oral-lateral expression, and Hedgehog signaling oral-medial expression. Analysis of mutants for , , and reveal that their oral-aboral expression domains are established independently of each other. We also identify enhancers regulating oral-aboral expression of and , with mutagenesis showing roles for Fgf-dependent ETS motifs in oral and Bmp-dependent E-box motifs in aboral enhancer activity, consistent with dependence of expression on the Bmp-dependent E-box factor Hand2. These findings reveal how triangulation of three major signaling pathways converge on distinct gene regulatory modules to establish distinct oral-aboral cell fate decisions in the developing jaw. - Source: PubMed
Publication date: 2026/04/08
Paulissen EricJunaid MunizaBrugger LylaChen Hung-JhenCrump J Gage - The generation of hepatocyte-like cells (HLCs) from human pluripotent stem cells (hPSCs) holds great promise for drug discovery and cell-based therapy for liver disease. However, current differentiation protocols are complicated and unstable, and the underlying gene regulatory mechanisms of hepatic differentiation remain incompletely defined. Here, we developed a machine learning-based artificial intelligence (AI) tool using phase-contrast images of hepatic progenitor cells (HPCs), which are essential for generating HLCs. The AI tool significantly improves the success rate of hepatic differentiation without the need for immunostaining or lineage tracing. By optimizing the methodology, we achieved an impressive purity of 90-95% for HLCs derived from hPSCs, aided by the AI algorithm. Through further investigating transcriptomes and epigenomic changes, we discovered the pivotal roles of NR5A2 and AP-1 transcription factors in regulating the maturation of hepatocytes. Single-cell RNA sequencing (scRNA-seq) demonstrated the upregulation of NR5A2 and AP-1 during hepatic differentiation. Importantly, mutagenesis and tumorigenesis assays confirmed the safety of this modified hepatic differentiation protocol. This work highlights the potential of combining AI algorithm and computational genomics to facilitate development of lineage differentiation and molecular mechanism study. - Source: PubMed
Publication date: 2026/04/08
Huo ZijunTu JianYang Wei-LeiHuang Mo-FanWang RuoyuChu Chih-WeiXu AnYu YaoTavakol Tara NKizilbash MikalFisher Megan EHuang Yu-WenZhu DandanPhan Trinh T TShoemaker RachelChen Ya-WenZhang YangHuff Chad DChen Shih-YuLiu Tien-JenXiao HaipengLee Dung-FangZhao Ruiying - The freshwater snail is both ecologically and economically significant, exhibiting notable sexual dimorphism in growth and nutritional traits that underscore the importance of breeding of monosex stocks. However, the genetic basis of sex determination remains unclear. Herein, genome-wide association studies (GWASs) combined with transcriptomic analysis were conducted to identify sex-linked markers and candidate genes for this species. GWAS generated 571 significantly sex-associated SNPs and 1853 InDels, corresponding to 44 candidate genes. Multiple significant SNP peaks were detected on chromosomes 1 and 2, with and as key candidate genes. A sex-linked InDel marker located within can distinguish males and females cost-effectively. Genotype analysis of the sex-associated loci revealed that most females were homozygous while males were heterozygous, suggesting that has a primarily XX/XY sex determination system. Comparative gonadal transcriptome analyses identified 2996 female-biased and 4281 male-biased genes. Among them, , , and may be critical in male sex differentiation, while , , and may be important in female sex differentiation. Integration of GWAS and transcriptomic data highlighted four pronounced sex-associated candidate genes, including , , and . These results provide a valuable foundation for elucidating the genetic mechanisms underlying sex determination and for the development of monosex stocks in . - Source: PubMed
Publication date: 2026/03/14
Gao YajunWen YanhongYi ShaokuiLin YongPeng JinxiaPan XianhuiZhou Xiaoyun - Intramuscular fat (IMF) content critically determines chicken meat quality. Extracellular vesicles (EVs) are essential mediators of intercellular communication through bioactive molecule transfer. Although co-culture systems have demonstrated that intramuscular preadipocytes (IMPs) promote lipogenic differentiation of muscle satellite cells (MSCs), the involvement of EVs remained unclear. Here, we isolated IMP-derived exosomes and confirmed their ability to enhance lipid accumulation in MSCs. Through small RNA sequencing and functional validation, we identified miR-125b-5p as a key regulator that directly targets and suppresses NR5A2, a newly identified negative regulator of avian adipogenesis. Inhibition experiments confirmed the essential role of this axis, as miR-125b-5p inhibition attenuated exosomal pro-adipogenic effects. Collectively, we demonstrated that IMP-derived exosomes facilitate MSC lipogenic differentiation through miR-125b-5p delivery. Our findings provide the first evidence of exosome-mediated muscle-fat crosstalk in poultry, establishing the miR-125b-5p/NR5A2 axis as a potential target for IMF modulation. - Source: PubMed
Publication date: 2026/03/10
Du JinmingKong LingzheCui JinyanJiang WenyueMa TianyiYang QingzhuHuang XinLiu DianhuiWang WeiyuSun Yingning