POLR2B antibody - N-terminal region (ARP32457_T100)
- Known as:
- POLR2B (anti-) - N-terminal region (ARP32457_T100)
- Catalog number:
- arp32457_t100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- POLR2B antibody - N-terminal region (ARP32457_T100)
Related genes to: POLR2B antibody - N-terminal region (ARP32457_T100)
- Gene:
- POLR2B NIH gene
- Name:
- RNA polymerase II subunit B
- Previous symbol:
- -
- Synonyms:
- RPB2
- Chromosome:
- 4q12
- Locus Type:
- gene with protein product
- Date approved:
- 1992-10-21
- Date modifiied:
- 2016-07-11
Related products to: POLR2B antibody - N-terminal region (ARP32457_T100)
Related articles to: POLR2B antibody - N-terminal region (ARP32457_T100)
- Chronic cadmium exposure is linked to esophageal squamous cell carcinoma (ESCC) progression, treatment resistance, and poor prognosis, but its molecular mechanisms remain unclear. Based on whole-transcriptome sequencing (RNA-seq) on chronic cadmium-treated (CCT) and untreated human EC109 ESCC cells, we performed lncRNA-mRNA co-expression and protein-protein interaction (PPI) network analyses to screen the hub genes related to cadmium exposure. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses depicted that these genes were mainly enriched in nucleocytoplasmic transport, ferroptosis, Huntington disease, and cellular senescence. Based on the lncRNA-miRNA-mRNA potential interaction, three lncRNAs (AC107068.1, TTN-AS1, MAPKAPK5-AS1) and ten mRNAs (IPO5, NUDCD1, OSTM1, CCNB1, FANCD2, TFRC, POLR2B, HTT, NUP43, NBN) were selected for competitive endogenous RNA (ceRNA) network construction. Connectivity map (CMap) analysis for the target mRNAs showed that JAK3-inhibitor-I was the most promising therapeutic drug for cadmium-induced ESCC progression. The prognostic value and abnormal expression of the candidate mRNAs were then validated in TCGA cohorts, cadmium-treated cancer cells and 41 ESCC specimens. Moreover, migration and invasion assays were performed to assess the effects of the identified gene on cell malignant phenotypes. As a result, IPO5 was identified as the pivotal gene and MAPKAPK5-AS1-hsa-miR-379-5p-IPO5 was deemed as a potential ceRNA regulatory mechanism for cadmium carcinogenesis in ESCC. Using TIMER and EPIC algorithms, IPO5 was correlated with increased infiltration of CD4+ T cells and macrophages, while negatively associated with CD8+ T cells and NK cells in ESCC tissues. Collectively, our study provides valuable information in understanding the molecular mechanisms involved in cadmium-induced ESCC progression and treatment resistance. Furthermore, we predict potential agents for cadmium carcinogenicity prevention and treatment. - Source: PubMed
Publication date: 2025/12/25
Zhu RuiChen JiongyuZhang XiarongChen QianJin YingmingZeng MingqinLuo JiananHuang YitengPeng Lin - 2,3,7,8-Tetrachlorodibenzo--dioxin (TCDD) is a toxic compound affecting organs like the liver, kidney, lung, and reproductive systems in mammals. This study outlines a strategy for choosing appropriate HKGs for tissue-specific gene expression analysis in TCDD toxicity, including four steps: i) identifying candidate HKGs from literature and databases; ii) defining primers from literature or designing new ones; iii) validating primer efficiency and specificity; iv) experimentally assessing candidate HKGs' stability in various tissues of TCDD-exposed mice. Based on this strategy, a total of 40 potential HKGs was selected, further filtered based on their database sources and ranked according to their frequency of use or expression stability. Ultimately, we identified a final set of 15 HKGs (, , , , , , , , , , , , , and ) with typical efficiencies for further evaluation. Then, the stability of the selected HKGs was determined in the liver, kidney, lung, ovary and testis of TCDD-exposed mouse compared to the control group using the [log (2)] and statistically analyzed using Pearson correlation coefficient () by BestKeeper algorithm. Our data analysis revealed that , , and were the most stable HKGs for normalizing gene expression in the liver, while , , and were suitable for kidney tissue. In the lung, , , and showed stability, while , , and were most stable in ovary. Lastly, , , and were accurately stable in the testis of TCDD-exposed mice. Our study identifies stable HKGs, improving TCDD toxicity research accuracy and reliability. - Source: PubMed
Publication date: 2025/04/27
Hammoudeh NourHasan ReemDeeb MohammadRadwan ZuherAyoubi OmarAlendary RoaaYoussef MouayadKazan AbdulfattahAlsahli RasilFaiad WalaaAldeli NourHanano Abdulsamie - Slow transit constipation (STC) is a complication of depression that can negatively impact patient prognosis and quality of life. Nonetheless, the pathogenesis of STC is unclear. In this work, colon tissues from STC and non-STC patients were utilized to determine transcriptome expression patterns (messenger ribonucleic acids [mRNAs], Long noncoding RNAs [lncRNAs], and Circular RNAs [circRNAs]) via high-throughput sequencing. We found that 4430 mRNAs, 984 lncRNAs, and 2152 circRNAs exhibited substantial variations in expression patterns in the colon tissues of STC and non-STC patients. Next, we constructed a protein-protein interaction network and identified three significant elements, namely, POLR2B, SRSF1, and SUMO1, which attracted our interest. Utilizing the data of 6 upregulated circRNAs and 10 downregulated circRNAs, we created a competing endogenous RNA network. Subsequently, we found that hsa_circ_0000994 and hsa_circ_0008699 were significantly enriched in the upregulated and downregulated networks, respectively. The coexpression network analysis suggested that circRNAs and lncRNAs might exert control over mRNAs by influencing the neural functions of STC. According to the results of the integrated circRNA-miRNA-mRNA network, circRNA-regulated mRNAs were linked to both the transforming growth factor-β (TGF-β) and Notch signaling pathways. Our findings could provide new perspectives for identifying potential prognostic markers in STC. Targeting SUMO1 may present a promising approach to address colonic motility disorders in STC therapy. - Source: PubMed
Publication date: 2025/04/15
Sun MingmingWang YahuiYang HuijuWang XiaopengSu LianlinYan Shuai - Asthma, a prevalent chronic inflammatory disorder, is shaped by a multifaceted interplay between genetic susceptibilities and environmental exposures. Despite strides in deciphering its pathophysiological landscape, the intricate molecular underpinnings of asthma remain elusive. The focus has increasingly shifted toward the metabolic aberrations accompanying asthma, particularly within the domain of pyrimidine metabolism (PyM)-a critical pathway in nucleotide synthesis and degradation. While the therapeutic relevance of PyM has been recognized across various diseases, its specific contributions to asthma pathology are yet underexplored. This study employs sophisticated bioinformatics approaches to delineate and confirm the involvement of PyM genes (PyMGs) in asthma, aiming to bridge this significant gap in knowledge. - Source: PubMed
Publication date: 2024/08/31
Zhang DihuiPu XiaoweiZheng ManLi GuanghuiChen Jia - Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disease. However, there is a paucity of studies that reflect the available biomarkers from separate gene expression profiles in PAH. - Source: PubMed
Publication date: 2024/08/03
Yang ChuangLiu Yi-HangZheng Hai-Kuo