LHX3 antibody - middle region (ARP32443_P050)
- Known as:
- LHX3 (anti-) - middle region (ARP32443_P050)
- Catalog number:
- arp32443_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- LHX3 antibody - middle region (ARP32443_P050)
Related genes to: LHX3 antibody - middle region (ARP32443_P050)
- Gene:
- LHX3 NIH gene
- Name:
- LIM homeobox 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 9q34.3
- Locus Type:
- gene with protein product
- Date approved:
- 2000-03-22
- Date modifiied:
- 2015-09-03
Related products to: LHX3 antibody - middle region (ARP32443_P050)
Related articles to: LHX3 antibody - middle region (ARP32443_P050)
- Bipolar cells relay visual signals from photoreceptors to ganglion cells. In the mouse retina, fifteen bipolar cell subtypes have been identified and are classified as ON or OFF bipolar cells based on their responses to light, or as rod or cone bipolar cells based on their photoreceptor connectivity. Despite this diversity, the distinct structural and functional roles of bipolar cell subtypes in visual information processing remain poorly understood, largely due to lack of tools and models for their characterization. In this study, we generated inducible Cre mouse lines driven by the promoters of , , and and crossed them with ChR2EYFP reporter mice to trace lineage and characterize bipolar cell subtypes in postnatal and adult mouse retinas. Following tamoxifen induction in adult male and female mice, ChR2EYFP expression was detected in type 2, 6, and 7 bipolar cells in the CreER line, type 1b, 2, and 6 bipolar cells in the CreER line, and type 2, 3, 4, and 5 bipolar cells in the CreER line. In addition, CreER activity was observed in cone photoreceptor cells. ChR2EYFP expression was also detected in other ON and OFF cone bipolar cells, as well as rod bipolar cells, when tamoxifen induction was performed in the postnatal mice. These inducible Cre lines enable genetic manipulation in retinal bipolar cell subtypes at different developmental time points, and serve as tools for elucidation of the mechanisms that control bipolar cell subtype development and function. Bipolar cells are central connectors of the outer and inner retina and initiate processing of complex visual information. Bipolar cells differentiate into more than fifteen subtypes during development, and their structural diversity has been well studied. However, the unique contribution of these subtypes to visual information processing is poorly understood due to inadequate tools. In this study, we develop and characterize three inducible Cre mouse models for temporally-defined genetic manipulation in bipolar cell subtypes in the developing and adult mouse retina. These models serve as tools to elucidate the mechanisms that regulate development and function of bipolar cell subtypes in the mouse retina. - Source: PubMed
Publication date: 2026/04/01
Quainoo Ebenezer JXie XiaolingKol LotemSamson JosephGan Lin - Efferocytosis can promote tumor immune evasion. This study identified three molecular subtypes and constructed a six-gene prognostic model for colon cancer based on efferocytosis-related genes and data from The Cancer Genome Atlas (TCGA). Patients in the high-risk group defined by this model showed higher levels of immunosuppressive cell infiltration and increased mutation rates, exhibiting poorer responses to immunotherapy. The model gene LHX3 was highly expressed in colon cancer, promoting malignant cellular phenotypes and influencing efferocytosis. Overall, the efferocytosis-based risk model constructed in this study may provide insights for prognostic evaluation and therapeutic strategies in colon cancer. - Source: PubMed
Publication date: 2026/03/21
Sun XiayanChen Fei - Bipolar cells relay visual signals from photoreceptor cells to ganglion cells in the retina. Fifteen bipolar cell subtypes have been identified in the mouse retina. These subtypes are classified as ON and OFF bipolar cells based on their response to light stimulus or rod and cone bipolar cells based on their connection to photoreceptor cells. However, the unique structural and functional role of these subtypes in the processing of visual information is not fully known due to the inadequate tools and models available for their characterization. In this study, to trace the lineage and characterize , , and - expressing bipolar cell subtypes in developing and adult mouse retina, we developed inducible Cre lines under the promoter of , , and and crossed them with ChR2EYFP reporter mice. Lineages of cells expressing , , and after Cre induction in the adult and postnatal mouse retina were then characterized. ChR2EYFP expression driven by -CreER was detected in type 2, 6, and 7 bipolar cells, -CreER in type 1b, 2, and 6 bipolar cells, and -CreER in type 2, 3, 4, and 5 bipolar cells as well as cone photoreceptor cells in the adult mouse retina. These bipolar cell subtype-specific inducible Cre mouse lines serve as efficient tools for elucidation of the mechanisms that control bipolar cell subtype development and function in the retina. - Source: PubMed
Publication date: 2025/12/30
Quainoo Ebenezer JXie XiaolingKol LotemSamson JosephGan Lin - Over 15% of cancers worldwide are caused by viruses. Merkel cell polyomavirus (MCPyV) is the most recently discovered human oncovirus and is the only polyomavirus that drives malignant tumors in humans. Here, we show that MCPyV+ Merkel cell carcinoma is defined by neuroendocrine-lineage core regulatory (CR) transcription factors (TFs) (ATOH1, INSM1, ISL1, LHX3, POU4F3, and SOX2) that were essential for tumor survival and that co-bound chromatin with the viral small T antigen at super enhancers. Moreover, MCPyV integration sites were enriched at these neuroendocrine super enhancers. We further discovered that the MCPyV noncoding control region contained a homeodomain binding motif absent in other polyomaviruses that bound ISL1 and LHX3 and depended on them for T antigen expression. To therapeutically target the CR factors, we used histone deacetylase (HDAC) inhibitors to collapse the chromatin architecture and induce topological blurring of superenhancer loops, abrogating core TF expression and halting tumor growth. To our knowledge, our study presents the first example of oncogenic cross-regulation between viral and human epigenomic circuitry to generate interlocking and essential transcriptional feedback circuits that explain why MCPyV causes neuroendocrine cancer and represent a tumor dependency that can be targeted therapeutically. - Source: PubMed
Publication date: 2025/12/15
Miao LinglingMilewski DavidCoxon AmyGelb TaraGarman Khalid APorch JadonKhanna ArushiCollado LorenHill Natasha TDaily KennethVilasi SerenaReed DanielleAlexander TiffanyStarrett Gabriel JChakraborty MaharshiSong YoungChoi RachelGangalapudi VineelaSeaman JosiahMorton AndrewBusam Klaus JVakoc Christopher RUrban Daniel JShen MinHall Matthew DSallari RichardKhan JavedGryder Berkley EBrownell Isaac - The hypothalamic-pituitary-ovarian (HPO) axis serves as the pivotal regulatory system governing reproduction in chickens. This study performed whole transcriptome sequencing on hypothalamus, pituitary, and ovarian tissues of Bian chickens to identify differentially expressed (DE) lncRNAs, miRNAs, and mRNAs ( < 0.05, FC > 2) between low- and high-laying groups. The hypothalamus exhibited 57 DE lncRNAs, 86 DE miRNAs, and 36 DE mRNAs; the pituitary showed the highest numbers with 206 DE lncRNAs, 234 DE miRNAs, and 528 DE mRNAs; while the ovary contained 111 lncRNAs, 230 miRNAs, and 62 mRNAs. GO functional enrichment analysis indicated that trans-target genes of hypothalamic and pituitary DE lncRNAs were enriched in cell proliferation Biological process (BP) terms (e.g., cell cycle, mitotic cell cycle). Hypothalamic miRNA targets clustered in metabolic regulation (cellular metabolic process), whereas pituitary miRNAs governed transport processes (nitrogen compound transport, intracellular transport). DE mRNAs showed BP terms enrichment in serotonin biosynthesis process, pituitary gland development, and DNA integration. KEGG pathway enrichment analysis revealed that lncRNA targets were significantly enriched in Progesterone-mediated oocyte maturation and Oocyte meiosis pathways in both hypothalamus and pituitary, with additional enrichment in Cell cycle and DNA replication. Notably, miRNA target genes showed conserved enrichment in metabolic regulation-related pathways (Metabolic pathways, Cysteine and methionine metabolism) across all three tissues. Key enriched pathways for DE mRNAs included Steroid biosynthesis, Cortisol synthesis and secretion, and Hippo signaling pathway. Finally, we constructed lncRNA-mRNA and miRNA-mRNA pairwise interaction networks, as well as ceRNA regulatory networks, through which we identified key regulatory networks targeting critical DE mRNAs, including and . These results elucidate the multi-tissue molecular mechanisms underlying egg-laying performance in chickens, providing novel targets for improving poultry reproductive efficiency through marker-assisted breeding. - Source: PubMed
Publication date: 2025/10/22
Li PeifengChu ChengzhuHu LijuanZhang GenxiWu PengfeiZhang Qi