SIRT5 antibody - middle region (ARP32391_T100)
- Known as:
- SIRT5 (anti-) - middle region (ARP32391_T100)
- Catalog number:
- arp32391_t100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- SIRT5 antibody - middle region (ARP32391_T100)
Related genes to: SIRT5 antibody - middle region (ARP32391_T100)
- Gene:
- SIRT5 NIH gene
- Name:
- sirtuin 5
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 6p23
- Locus Type:
- gene with protein product
- Date approved:
- 2001-03-20
- Date modifiied:
- 2014-11-19
Related products to: SIRT5 antibody - middle region (ARP32391_T100)
Related articles to: SIRT5 antibody - middle region (ARP32391_T100)
- Consumption of var. has antioxidant and hypoglycemic effects. Regarding the former, certain signaling pathways that influence these effects have already been proposed; however, the underlying molecular mechanisms of the hypoglycemic effects remain unknown. It has been recognized that the sirtuin-mediated signaling cascade responds to various stressors, such as oxidative stress, and regulates glucose metabolism. Therefore, it would be of great interest to determine whether there is a link between these two properties and whether it is mediated by sirtuins. Hence, the present study aimed to evaluate the effect of on the gene expression of the sirtuin family (SIRT1-SIRT6) in individuals with type 2 diabetes mellitus (T2DM). A quasi-experimental study was conducted with a convenience sample of 26 older adults diagnosed with T2DM, divided into a (i) placebo group (PG; = 14) and (ii) experimental group (EG; = 12). Clinical, biochemical, and anthropometric measurements were performed, and total oxidant/antioxidant capacity (TOS/TAS) and mRNA expression of genes encoding sirtuins were determined. All parameters were measured at baseline, three months, and six months after the intervention. In the EG, the expression levels of SIRT1, SIRT3, SIRT5, and SIRT6 increased by 52%, 69%, 62%, and 69%, respectively, six months after treatment. A 50% decrease in TOS and a 44% increase in TAS were also observed. Our findings suggest that the bioactive components of enhance sirtuin expression and exhibit antioxidant effects in older adults with T2DM. - Source: PubMed
Publication date: 2026/04/02
Gavia-García GracielaHernández-Álvarez DavidArista-Ugalde Taide LauritaAguiñiga-Sánchez ItzenSantiago-Osorio EdelmiroCadena-Iñiguez JorgeRosado-Pérez JuanaMendoza-Núñez Víctor Manuel - To investigate the role of the SIRT5-PRDX6 axis during the pathogenesis of sepsis-associated acute kidney injury (SA-AKI). - Source: PubMed
Publication date: 2026/04/10
Lin WenlongDong CaitaoJiang QinhongLou YuanquanWang LongHe Ziqi - Fat deposition plays a crucial role in regulating the production performance and meat quality of broilers. Although the heterogeneity of mammalian adipocytes has been extensively studied, research on the molecular mechanisms underlying differences in lipid droplet accumulation in avian adipocytes remains limited. This study confirmed a significant positive correlation (R > 0.81, < 0.001) between the SSC signal and lipid droplet content via fluorescence staining of lipid droplets, Oil Red O staining, and triglyceride (TG) quantification. Based on this, a label-free sorting strategy using SSC signals was established to sort differentiated chicken preadipocytes, obtaining high lipid droplet (H) and low lipid droplet (L) subpopulations, which were subsequently subjected to transcriptome sequencing and differential gene expression (DEG) analysis, followed by GO and KEGG enrichment analysis. The results indicated no significant differences in the expression of adipogenesis marker genes (, , , , ) between the high lipid droplet (H) and low lipid droplet (L) groups, suggesting that both groups are at similar stages of differentiation. KEGG analysis revealed that both the H vs. NC and L vs. NC comparisons were enriched in common pathways, including the PPAR signaling pathway, ECM-receptor interaction, focal adhesion, cytokine-receptor interaction, and calcium-Apelin signaling pathway, suggesting that both groups of cells had activated the adipogenesis program. GO analysis showed that, in both H vs. NC and L vs. NC comparisons, differentially expressed genes (DEGs) were enriched in biological processes (BPs) related to cell adhesion, nucleosome assembly, chromatin remodeling, and receptor activity, as well as cellular components (CCs) such as the extracellular matrix, cytoskeleton, and nucleosome organization, indicating extensive gene reprogramming and activation of signaling transduction during differentiation. In the H vs. L comparison, enriched pathways included ABC transporters, ECM-receptor interaction, focal adhesion, gap junctions, microtubule-related processes, and neuroactive ligand-receptor interactions, involving lipid transmembrane transport, cytoskeleton stabilization, and signal transduction regulation, suggesting that high lipid droplet cells are more mature in lipid droplet transport, storage, and homeostasis maintenance. GO enrichment results further supported this conclusion, as H vs. L specifically enriched processes related to microtubule-related processes, cell cycle, and redox reactions (BPs), as well as chromosome organization, cytoskeleton, and motor activity (CC/MF), indicating that high lipid droplet cells maintain lipid droplet fusion and metabolic homeostasis via enhanced microtubule transport and antioxidant regulation. Differential gene analysis revealed that the L group upregulated genes associated with fatty acid synthesis and elongation (, , , , ), cholesterol and isoprenoid biosynthesis (, , , , , , ), and fatty acid oxidation (, , , ), reflecting a metabolic characteristic of concurrent lipid synthesis and mobilization; the H group, conversely, upregulated genes associated with lipid droplet formation and storage (, , , , ), lipid transport (, , , , ), and antioxidant defense (, , ), exhibiting a storage and homeostasis-oriented metabolic state. In the NC, L, and H groups, the expression of five genes-, , , , and -showed a gradual increase, suggesting that these genes were associated with preadipocyte differentiation and lipid droplet deposition. In summary, although the high and low lipid droplet subpopulations of chicken preadipocytes exhibit similar differentiation states, they form distinct metabolic orientations. The L group is characterized by active lipid synthesis, fatty acid oxidation, and membrane lipid remodeling, while the H group predominantly features lipid droplet storage, lipid transport, and antioxidant homeostasis. This study highlights the molecular mechanisms underlying the metabolic heterogeneity of avian adipocytes and provides a theoretical basis for poultry fat deposition regulation and genetic improvement. - Source: PubMed
Publication date: 2026/03/12
Wang BoyuLi YantaoWang YakeChen JiayiWang JialiLi XiaopingLi Zhenhui - Despite adequate glycaemic control, diabetic complications frequently progress, underscoring how persistently protein post‑translational modifications (PTMs) contribute to disease pathology by sustaining 'metabolic memory'. Lysine succinylation, a PTM derived from the tricarboxylic acid cycle intermediate succinyl‑CoA and primarily regulated by the desuccinylase sirtuin 5 (SIRT5), has emerged as a key metabolic modulator. By introducing a marked shift in lysine charge, succinylation can notably influence enzyme activity and protein stability. The present review integrates current evidence associating the disruption of the succinyl‑CoA/SIRT5 regulatory axis with impaired metabolic flexibility in diabetes. The mechanisms by which pathological hypersuccinylation compromises mitochondrial bioenergetics, particularly by inhibiting uncoupling protein 1 in obesity and the pyruvate dehydrogenase complex in diabetic cardiomyopathy, are described, and its implications in neurodegeneration within diabetic retinopathy through modification of optineurin are elucidated. The present review also discusses the mechanistic role of epigenetic dysregulation, highlighting how activation of the lysine acetyltransferase 2A/H3K79 succinylation/spermidine/spermine N1‑acetyltransferase family member 2 pathway promotes ferroptosis and inflammation in diabetic kidney disease. The context‑dependent duality of SIRT5 function is also examined; although key in limiting lipotoxicity in cardiomyocytes and podocytes, SIRT5 can paradoxically aggravate glomerular fibrosis in renal mesangial cells by suppressing p53 signalling. The present findings suggested that re‑establishing succinylation homeostasis represents not simply a metabolic correction but a strategic therapeutic objective. However, given the tissue‑specific and frequently opposing effects of SIRT5, future therapeutic approaches should aim to emphasize organ‑targeted delivery rather than systemic modulation to minimize off‑target toxicity while effectively addressing diabetic complications. - Source: PubMed
Publication date: 2026/03/27
Xiong YetengLuo FeiLi BingnanYang Qinfeng - Cardiovascular diseases (CVDs) are the leading cause of global mortality and disease burden, with pathogenesis involving metabolic remodelling and inflammatory responses. Metabolic remodelling is also increasingly regarded as a key factor in the pathogenesis of CVDs, leading to the abnormal accumulation of metabolic intermediates. Current research has shown that succinate, an intermediate product of the tricarboxylic acid cycle (TCA), significantly accumulates in CVDs, promoting the occurrence of disease. In recent years, the role of epigenetics in CVDs has received increasing attention. Compared with its modification, succinylation modification directly links metabolism and epigenetics. Therefore, in CVDs where metabolic remodelling is a key factor, succinylation modification may play a crucial role. This article describes the role of succinate and succinylation modifications in CVDs. For example, in ischaemia‒reperfusion injury (IRI), the abnormal accumulation of succinate during ischaemia and the oxidation of succinate by succinylation dehydrogenase (SDH) during reperfusion lead to the production of large amounts of reactive oxygen species (ROS), and the succinylation modification of carnitine palmitoyltransferase 2 (CPT2) at lysine 424 increases the lipotoxicity to diabetic hearts. In addition, given the significant role of succinate and succinylation modifications in the cardiovascular system, we focused on therapeutic strategies targeting succinate and succinylation modifications, including inhibitors of SDH, inhibitors of succinate receptor 1 (SUCNR1), and activators of sirtuin 5 (SIRT5), providing new insights for CVD intervention. - Source: PubMed
Publication date: 2026/03/23
Liu WenyanHan YuZhang FengquanLeng ShuaiYu Wenqian