MYF5 antibody - N-terminal region (ARP32134_P050)
- Known as:
- MYF5 (anti-) - N-terminal region (ARP32134_P050)
- Catalog number:
- arp32134_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- MYF5 antibody - N-terminal region (ARP32134_P050)
Related genes to: MYF5 antibody - N-terminal region (ARP32134_P050)
- Gene:
- MYF5 NIH gene
- Name:
- myogenic factor 5
- Previous symbol:
- -
- Synonyms:
- bHLHc2
- Chromosome:
- 12q21.31
- Locus Type:
- gene with protein product
- Date approved:
- 1989-05-25
- Date modifiied:
- 2016-10-05
Related products to: MYF5 antibody - N-terminal region (ARP32134_P050)
Related articles to: MYF5 antibody - N-terminal region (ARP32134_P050)
- This study evaluated how embryonic choline supplementation (ECS) via in ovo injection and gosling choline supplementation (GCS) via diet jointly influence embryonic development, endocrine status, growth performance, and muscle development. A total of 1,250 hatching eggs were allocated to five ECS treatments: non-injected control (E), sham-injected control (E), and 0.5 mL of choline chloride at 0.5 (E), 1.0 (E), and 1.5 (E) mg/mL. Following hatch, 720 female goslings (144 per ECS group) were randomly assigned to three GCS levels: 0 (G), 200 (G), and 400 (G) mg/kg. ECS significantly increased embryo weight and relative embryo weight (embryo weight as a percentage of initial egg weight) at embryonic day 18 (DE18; P < 0.001) and DE27 (P = 0.005), while no significant effects were observed at DE20 (P > 0.05). Circulating growth hormone (GH), adrenocorticotropic hormone (ACT), and corticosterone (CORT) responded to choline in dose-dependent patterns (P < 0.001). Post-hatch body weight was significantly affected by the ECS × GCS interaction throughout the post-hatch period (P ≤ 0.016 at 7, 14, 21, and 28 days of age). GCS at 400 mg/kg significantly improved 28-day body weight (P < 0.001) and feed conversion ratio (P < 0.001). Pectoralis and leg muscle myofiber diameter and cross-sectional area were enhanced by higher choline supply (ECS: P ≤ 0.041; GCS: P < 0.001), accompanied by reduced pectoral fiber density (P < 0.001). Notably, myofiber hypertrophy occurred without changes in myogenin (MyoG), myogenic regulatory factor 4 (MRF4), or myogenic factor 5 (Myf5) expression (P > 0.05). Instead, choline exerted treatment-dependent modulation of IGF-1 and MSTN primarily in the pectoralis (P < 0.001), indicating that choline promotes muscle growth via the IGF-1/MSTN axis. For growth rate and feed efficiency, EG is preferable; for muscle yield, EG offers additional fiber gains; for immune organ development, EG is preferable given the increased bursa weights at lower supplementation. These findings provide a scientific basis for targeted choline nutritional strategies in goose production systems. - Source: PubMed
Publication date: 2026/05/01
Liu WenfengZheng XuchengLi XuanYang HaimingWang Zhiyue - Our previous research indicated that monochromatic light combinations influence muscle fiber types in broilers, potentially through alterations in thyroid function, yet the underlying mechanisms remained elusive. Here, we employed a thyroidectomy (TX) model to investigate the role of thyroid hormone (T3) in mediating these light-induced effects. Broilers were reared under single-monochromatic light (white-white [WW], red-red [RR], green-green [GG], blue-blue [BB]) or different light combinations (green-blue [GB], blue-green [BG], red-blue [RB]). Our results demonstrate that monochromatic light combinations modulate serum thyroid hormone (TH) levels, thereby regulating oxidative stress (OS) and the composition of slow- (MyHC-I/IIa) and fast-twitch (MyHC-IIb) fibers in the gastrocnemius (GAS). Critically, TX-induced hypothyroidism not only reduced malondialdehyde (MDA) levels and the proportion of fast glycolytic fibers (MyHC-IIb) but also promoted a shift toward oxidative fibers (MyHC-I/IIa). Notably, this TX intervention abolished the intergroup differences in fiber-type composition caused by different light colors, confirming the indispensable mediating role of TH. Mechanistically, we found that TX significantly upregulated the inactivating deiodinase DIO3-a direct transcriptional target of the β-Catenin/TCF complex-while downregulating the activating deiodinase DIO2 and thyroid hormone receptor α (TRα). This was accompanied by enhanced phosphorylation of the PI3K/AKT/GSK3β axis, increased β-Catenin protein levels, and corresponding changes in gene expression: upregulation of slow-fiber markers (Pax7, Myf5, MYH7B) and downregulation of fast-fiber markers (MyoD, MyoG, Mstn, MYH1A). In conclusion, our study reveals that deiodinases DIO2 and DIO3 are critical mediators through which monochromatic light regulates intramuscular T3 levels and oxidative stress, driving fiber-type transitions via the PI3K/AKT-Wnt/β-Catenin signaling pathway. - Source: PubMed
Publication date: 2026/05/20
Liu YamengWang ZixuCao JingDong YulanGao TingLi FuweiSong EnliangChen Yaoxing - In the present study, myoblast determination protein (myod) and myogenic factor 5 (myf5), two critical primary myogenic regulatory factors (MRFs) that govern muscle growth were characterized in snow trout (Schizothorax richardsonii). In addition, their mRNA expression was examined in relation to age, sex, nutritional conditions and temperature regimes. The full-length of Srmyod and Srmyf5 mRNA sequences were 1638 and 1397 nucleotides, respectively; with open reading frames of 825 and 723 nucleotides, which encoded proteins of 273 and 240 amino acid residues. In silico protein-protein interaction, nuclear localization signals, ligand-binding residues and tertiary protein structures indicated their putative roles in dimerization, DNA binding, and transcriptional activation. With respect to muscle mRNA expression, Srmyod was upregulated in older fish (2+ age) and conversely Srmyf5 was higher in younger fish (0+ age). There were no sex-dependent differences in MRFs expression. Three weeks of refeeding following three weeks of starvation significantly upregulated the transcriptional levels of both MRFs, corroborating the compensatory growth potential of the fish. The expression of Srmyf5 and Srmyod were concurrently high in fish fed 35% dietary protein. In contrast, dietary lipid levels (3-13%) had no significant effect on MRF expression. With respect to rearing temperature (6-24 °C), Srmyf5 expression showed marked upregulation at 18 °C, consistent with optimal fish growth. Overall, these findings reveal the conserved molecular profile and distinct transcriptional regulation of myod and myf5 in snow trout, under different biological, nutritional and environmental determinants of growth. - Source: PubMed
Publication date: 2026/05/23
Rajesh ManchiKamalam Biju SamBaral PratibhaMahija JettiboinaCiji AlexanderAkhtar Md ShabhazSharma PrakashSingh Atul Kumar - The analytical accuracy of gene expression analysis largely depends on the stability of the reference gene(s) used for normalization of target mRNA expression under different experimental conditions. In this study, we characterized three reference genes: beta-actin (β-act), elongation factor 1 alpha (ef1α), and glyceraldehyde 3-phosphate dehydrogenase (gapdh) in the Trans-Himalayan snow trout, Schizothorax richardsonii. Subsequently, we evaluated their stability, along with 18 S rRNA, under eight experimental conditions using the geNorm, NormFinder, BestKeeper, and delta CT algorithms. The results indicated that 18 S rRNA was the most stable reference gene across sixteen tissue types, whereas ef1α showed the highest stability during early developmental stages (0-21 days post-hatch) and between sexes (male and female muscle tissue). In other instances, β-act was the most stable reference gene in snow trout muscle across different age groups (0 + and 2 + years), nutritional conditions (starvation-refeeding and varying dietary protein and lipid levels), and rearing temperatures (6, 12, 18, and 24 °C). In contrast, gapdh exhibited poor stability and was regulated by low temperature, making it unsuitable as a reference gene under such conditions. For further validation, we performed relative quantification of primary myogenic regulators (myod and myf5) at different rearing temperatures using the most and least stable reference gene(s) as normalizers. The results reaffirmed the importance of selecting stable reference gene(s) for qRT-PCR assays, as the expression patterns of the myogenic regulators were significantly incorrect when normalized using the least stable reference genes. To our knowledge, this is the first comprehensive evaluation of internal reference genes for gene expression analysis in a coldwater cyprinid across different tissues, developmental stages, age, sex, nutritional conditions and temperature regimes. - Source: PubMed
Publication date: 2026/05/16
Rajesh ManchiKamalam Biju SamJoshi RiniAlexander CijiAkhtar Md ShabazSharma Prakash - Studies examining the paracrine effect of fibroblasts on the myogenesis of bovine muscle satellite cells (MuSCs) have confirmed their stimulating effect on proliferation and early differentiation. However, traditional two-dimensional (2D) cell culture models fail to accurately represent the complexity of in vivo muscle tissue. This study aims to investigate the paracrine effect of fibroblasts on myogenesis in a three-dimensional (3D) cell culture model. Cells were cultured in monoculture and co-culture with fibroblasts in a spinner flask using gelatin microcarriers, Cultishper, which maximizes the growth surface area for adherent cells. Then, muscle cells from the co-culture were sorted by the FACS method based on negative expression of CD90, a fibroblast-associated marker. The progress of myogenesis was assessed based on qPCR analysis for selected muscle markers and at the protein level for skeletal myosin. Fluorescent staining and luminescent metabolic assays were performed to control culture conditions. The obtained results revealed up-regulation of genes involved in cell activation and proliferation (PAX7, MYF5, and MYOD) and differentiation (MYOG, EGR1, and MYH). Metabolism analyses did not show changes between mono- and co-culture conditions. To summarize, fibroblasts through paracrine signaling promote early differentiation of MuSCs and potentially proliferation, which provides valuable insights for the advancement of cultured meat production. - Source: PubMed
Publication date: 2026/05/11
Zygmunt KarolinaPiórkowska KatarzynaAdamiak JuliaWitarski Wojciech