GSX1 antibody - N-terminal region (ARP31662_P050)
- Known as:
- GSX1 (anti-) - N-terminal region (ARP31662_P050)
- Catalog number:
- arp31662_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- GSX1 antibody - N-terminal region (ARP31662_P050)
Related genes to: GSX1 antibody - N-terminal region (ARP31662_P050)
- Gene:
- GSX1 NIH gene
- Name:
- GS homeobox 1
- Previous symbol:
- GSH1
- Synonyms:
- Gsh-1
- Chromosome:
- 13q12.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-02-18
- Date modifiied:
- 2014-11-18
Related products to: GSX1 antibody - N-terminal region (ARP31662_P050)
Related articles to: GSX1 antibody - N-terminal region (ARP31662_P050)
- The specification and maintenance of human forebrain neural progenitor cells (NPCs) depend on both intrinsic gene networks and spatially localized niche signals, but the interplay between these cues remains incompletely understood. Here, we identify a spatially organized, paracrine IGF1 signaling architecture that regulates human FOXG1 NPCs through multilayered transcriptional and translational control. Using a pluripotent stem cell-derived forebrain model, we show that FOXG1 NPCs express IGF1 receptors but lack endogenous IGF1, instead depending on neighboring epithelial-like domains that secrete IGF1. IGF1 promotes progenitor proliferation, clonal expansion, and vertical tissue growth by activating PI3K-AKT-mTOR and MEK-ERK pathways. Ribosome profiling and 5'UTR reporter assays reveal that mTOR signaling selectively enhances translation of neurodevelopmental and biosynthetic transcripts- including , a ventral fate determinant implicated in interneuron specification and autism. These findings uncover a human-specific regulatory mechanism in which spatially restricted IGF1-mTOR signaling integrates niche signals with translational output to support progenitor identity, biosynthetic capacity, and developmental resilience. - Source: PubMed
Publication date: 2025/09/24
Lisst KadiaHuo DaEacker Stephen MZhang SonyaYang AlinaHuang YuejiaDawson Ted MDawson Valina LXu Jin-Chong - While significant progress has been made in understanding the heterogeneity in the NSCs, our understanding of similar heterogeneity among the more abundant transit amplifying progenitors is lagging. Our work on the NPs of the neonatal subventricular zone (SVZ) began over a decade ago, when we used antibodies to the 4 antigens, Lex CD133,LeX,CD140a and NG2 and FACs to classify subsets of the neontal SVZ as either multi-potential (MP1, MP2, MP3, MP4 and PFMPs), glial-restricted (GRP1, GRP2, and GRP3), or neuron-astrocyte restricted (BNAP). Using RNAseq we have characterized the distinctive molecular fingerprint of 4 SVZ neural progenitors and compared their gene expression profiles to those of the NSCs. Overall, we identified 1581 genes that were upregulated in at least one NP compared to the NSCs. Of these genes, 796 genes were upregulated in BNAP/GRP1 compared to NSCs; 653 in GRP2/MP3; 440 in GRP3; 527 in PFMPs. One gene in particular that emerged from our analysis that can be used to distinguish the NPs from the NSCs is Etv1, also known as Er81. Interestingly, PFMPs expressed high levels of the transcription factor GSX1, which has been shown to play important roles in regulating interneuron development in the forebrain. PFMPs are also highly express PDGFRα, CSPG4 (NG2+) Olig2 and transcripts for olfactory receptors. PFMPs also express Sox10, Gjb1, Zfp488 and Myt1 which have been shown to be important for oligodendrocyte development. The top transcription factors with unique upregulation in BNAP/GRP1s were Zfp57, Hoxac6, and Zfp955a. Unlike the other NPs, the GRP1 and GRP2 NPs expressed many proteins involved in immune cell function. In contrast, the top downregulated genes in the NPs are those involved in cilia formation, consistent with the loss of cilia as neural stem cells become multipotential progenitors. We performed bionformatic analyses to provide insights into the transcription factor interactions that are likely regulating their development as well as the functional consequences of these diffferences in gene expression. The present work will serve as an important resource for investigators interested in further defining the transit amplifying progenitors of the mammalian SVZ. - Source: PubMed
Publication date: 2025/08/31
Zaritsky RebeccaKumari EktaVelloso Fernando JanscurLemenze AlexanderHusain SeemaLevison Steven W - : Despite current treatments extending the lifespan of Glioblastoma (GBM) patients, the average survival time is around 15-18 months, underscoring the fatality of GBM. This study aims to investigate the impact of sample heterogeneity on gene expression in GBM, identify key metabolic pathways and gene modules, and explore potential therapeutic targets. : In this study, we analysed GBM transcriptome data derived from The Cancer Genome Atlas (TCGA) using genome-scale metabolic models (GEMs) and co-expression networks. We examine transcriptome data incorporating tumour purity scores (TPSs), allowing us to assess the impact of sample heterogeneity on gene expression profiles. We analysed the metabolic profile of GBM by generating condition-specific GEMs based on the TPS group. : Our findings revealed that over 90% of genes showing brain and glioma specificity in RNA expression demonstrate a high positive correlation, underscoring their expression is dominated by glioma cells. Conversely, negatively correlated genes are strongly associated with immune responses, indicating a complex interaction between glioma and immune pathways and non-tumorigenic cell dominance on gene expression. TPS-based metabolic profile analysis was supported by reporter metabolite analysis, highlighting several metabolic pathways, including arachidonic acid, kynurenine and NAD pathway. Through co-expression network analysis, we identified modules that significantly overlap with TPS-correlated genes. Notably, and are upregulated in High TPS, show a high correlation with TPS, and emerged as promising therapeutic targets. Additionally, exhibits a high centrality score within the co-expression module, which shows a positive correlation with TPS. Moreover, , an immune-related gene expressed in the brain, showed a negative correlation and upregulated in Low TPS, highlighting the importance of modulating immune responses in the GBM mechanism. : Our study uncovers sample heterogeneity's impact on gene expression and the molecular mechanisms driving GBM, and it identifies potential therapeutic targets for developing effective treatments for GBM patients. - Source: PubMed
Publication date: 2024/10/01
Kaynar AliKim WoongheeCeyhan Atakan BurakZhang ChengUhlén MathiasTurkez HasanShoaie SaeedMardinoglu Adil - Cervical cancer (CC) screening in women comprises human papillomavirus (HPV) testing followed by cytology triage of positive cases. Drawbacks, including cytology's low reproducibility and requirement for short screening intervals, raise the need for alternative triage methods. Here we used an innovative triage technique, the WID-qCIN test, to assess the DNA methylation of human genes DPP6, RALYL and GSX1 in a real-life cohort of 28,017 women aged ≥30 years who attended CC screening in Stockholm between January and March 2017. In the analysis of all 2,377 HPV-positive samples, a combination of WID-qCIN (with a predefined threshold) and HPV16 and/or HPV18 (HPV16/18) detected 93.4% of cervical intraepithelial neoplasia grade 3 and 100% of invasive CCs. The WID-qCIN/HPV16/18 combination predicted 69.4% of incident cervical intraepithelial neoplasia grade 2 or worse compared with 18.2% predicted by cytology. Cytology or WID-qCIN/HPV16/18 triage would require 4.1 and 2.4 colposcopy referrals to detect one cervical intraepithelial neoplasia grade 2 or worse, respectively, during the 6 year period. These findings support the use of WID-qCIN/HPV16/18 as an improved triage strategy for HPV-positive women. - Source: PubMed
Publication date: 2024/06/04
Schreiberhuber LenaBarrett James EWang JiangrongRedl ElisaHerzog ChiaraVavourakis Charlotte DSundström KarinDillner JoakimWidschwendter Martin - As essential components of gene expression networks, transcription factors regulate neural circuit assembly. The homeobox transcription factor encoding gene, gs homeobox 1 (gsx1), is expressed in the developing visual system; however, no studies have examined its role in visual system formation. In zebrafish, retinal ganglion cell (RGC) axons that transmit visual information to the brain terminate in ten arborization fields (AFs) in the optic tectum (TeO), pretectum (Pr), and thalamus. Pretectal AFs (AF1-AF9) mediate distinct visual behaviors, yet we understand less about their development compared to AF10 in the TeO. Using gsx1 zebrafish mutants, immunohistochemistry, and transgenic lines, we observed that gsx1 is required for vesicular glutamate transporter, Tg(slc17a6b:DsRed), expression in the Pr, but not overall neuron number. gsx1 mutants have normal eye morphology, yet they exhibit impaired visual ability during prey capture. RGC axon volume in the gsx1 mutant Pr and TeO is reduced, and AF7 that is active during feeding is missing which is consistent with reduced hunting performance. Timed laser ablation of Tg(slc17a6b:DsRed)-positive cells reveals that they are necessary for AF7 formation. This work is the first to implicate gsx1 in establishing cell identity and functional neural circuits in the visual system. - Source: PubMed
Publication date: 2024/04/26
Schmidt Alexandra RPlacer Haiden JMuhammad Ishmael MShephard RebekahPatrick Regina LSaurborn TaylorHorstick Eric JBergeron Sadie A