RGS4 antibody - C-terminal region (ARP30213_P050)
- Known as:
- RGS4 (anti-) - C-terminal region (ARP30213_P050)
- Catalog number:
- arp30213_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- RGS4 antibody - C-terminal region (ARP30213_P050)
Related genes to: RGS4 antibody - C-terminal region (ARP30213_P050)
- Gene:
- RGS4 NIH gene
- Name:
- regulator of G protein signaling 4
- Previous symbol:
- SCZD9
- Synonyms:
- -
- Chromosome:
- 1q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1997-06-09
- Date modifiied:
- 2017-04-13
Related products to: RGS4 antibody - C-terminal region (ARP30213_P050)
Related articles to: RGS4 antibody - C-terminal region (ARP30213_P050)
- The present study aimed to explore the difference and its underlying molecular mechanism of in ovo feeding (IOF) with Codonopsis pilosula polysaccharides (CPPS) on skeletal muscle in ducks. A total of 120 fertile eggs were randomly distributed into two treatment groups, with 6 replicates per group and 10 eggs per replicate. IOF was performed at incubation day 13 (I13). The control group was injected with 0.2 mL 0.75% sterile saline, while the CPPS group received 0.2 mL CPPS solution at a dose of 3 mg per egg. Breast muscle and leg muscle tissue samples were collected at I24 for multi-omics (transcriptomics, metabolomics and proteomics) analyses. In breast muscle, a total of 1301 differentially expressed genes (DEGs) were identified (497 upregulated and 804 downregulated). Genes involved in muscle development, such as creatine kinase, mitochondrial 2 (CKMT2, ENSAPLG00020008721) and G-protein signaling 4(RGS4, ENSAPLG00020014659), were significantly upregulated. For proteomics, 89 different expressed proteins (DEPs) were identified (44 upregulated and 45 downregulated), among which, Eukaryotic translation initiation factor 4E type 2 (eIF4E2, A0A8B9SQK7), myomesin 3 (A0A8B9ZBZ5), myogenic factor 6 (A0A8B9SGC1), myosin heavy chain and other myogenic-related proteins (MyHC, A0A8B9ZL15) related to muscle synthesis were markedly increased. Metabolomic analysis identified 1647 differential metabolites (DEMs), including 43 upregulated and 22 downregulated, with leucine being significantly decreased. Multi-omics integration revealed that these differential molecules were collectively enriched in the mTOR signaling pathway and glycerophospholipid metabolism pathway. These coordinated regulatory effects synergistically promoted breast muscle fiber development, enhanced energy metabolism, alleviated stress induced injury and ultimately improved skeletal muscle growth potential and meat quality. While in the leg muscles, 1195 DEGs were identified (487 upregulated and 708 downregulated), among which the expression of transcription factor 15 (TCF15, ENSAPLG00020006012), a gene associated with muscle development, was significantly upregulated. Proteomic analysis revealed 47 DEPs (15 upregulated and 32 downregulated), including phosphorus receptor protein (A0A8B9TCV3, PLN), mitofusin 1(A0A8B9TRP9, MFN1), titin(A0A8B9ZFJ8), which are closely related to muscle development and energy metabolism. Metabolomic analysis identified 1647 DEMs were identified (66 upregulated and 40 downregulated), with 5-Hydroxy-L-tryptophan (5-HTP), the precursor of serotonin (5-HT), significantly upregulated, providing substrates and energy for muscle development. Although no shared enriched pathway existed across the three omics in leg muscle, the differential molecules collectively suggest that IOF with CPPS supports energy supply for cell proliferation and creates a favorable environment for myogenesis. These findings help to elucidate the molecular mechanisms by which CPPS regulates embryonic skeletal muscle development. Moreover, the candidate genes, proteins and metabolites identified in this study may serve as valuable biomarkers and provide a potential nutritional strategy for improving meat quality traits in duck breeding. - Source: PubMed
Publication date: 2026/08/09
Liu AiRao YifuYue YongZhu JinjinWen JiyingYao BiqiongZhu YongcaiBoonanuntan SurintornYang Shenglin - Angiogenesis is critical for post-ischemic tissue repair. Although miR-485-5p is highly expressed in vascular tissues and RGS4 regulates angiogenesis, their specific roles under hypoxia remain largely unknown. This study aimed to elucidate these functions and underlying mechanisms. Using a CoClâ‚‚-induced hypoxic model in HUVECs, we observed that hypoxia significantly downregulated miR-485-5p expression while concurrently upregulating RGS4 mRNA levels. Notably, either inhibition of RGS4 or overexpression of miR-485-5p markedly enhanced HUVEC proliferation, migration, and tube formation. A luciferase reporter assay further confirmed that miR-485-5p directly targets RGS4. In addition, miR-485-5p overexpression or RGS4 inhibition increased the phosphorylation levels of VEGFR-2, AKT, and ERK1/2. Through co-immunoprecipitation assays, we revealed for the first time a direct interaction between RGS4 and VEGFR-2, which suppresses VEGF signaling and consequently impairs angiogenesis. Conversely, overexpression of RGS4 reversed the pro-angiogenic effects of miR-485-5p, as evidenced by reduced VEGFR-2/AKT/ERK1/2 phosphorylation and decreased proliferation, migration, and tube formation in HUVECs. These findings indicate that miR-485-5p promotes angiogenesis by downregulating RGS4. Consistent with these in vitro results, experiments in vivo using a rat hindlimb ischemia model confirmed that inhibition of RGS4 via si-RGS4 or overexpression of miR-485-5p via agomir-miR-485-5p markedly improved blood perfusion recovery, enhanced vascular angiogenesis, and increased VEGFR-2/AKT/ERK1/2 phosphorylation. Collectively, our study demonstrates that the miR-485-5p/RGS4 axis promotes hypoxia-induced angiogenesis by relieving RGS4-mediated inhibition of VEGFR-2/AKT/ERK1/2 signaling, thereby providing a strong rationale for targeting miR-485-5p as a possible treatment approach for lower extremity arterial disease. - Source: PubMed
Publication date: 2026/08/13
Li MengtingLuo YulinLi YufeiYou JingcanWang LiqunWu JianboZheng YoukunLuo Mao - The SNPs in the 5' regulatory region of the human RGS4 gene were reportedly associated with schizophrenia risk. - Source: PubMed
Publication date: 2026/08/01
Xu Feng-LingYang Xin-RuiYang Yan-YanWang Rong-ShuaiLiu Li - Breast cancer (BC) is a heterogeneous malignancy with diverse molecular subtypes and variable clinical outcomes. Despite diagnostic and therapeutic advances, recurrence and metastasis contribute to poor prognosis in subsets of patients. Regulators of G protein signaling (RGS) proteins, negative modulators of G protein-coupled receptor (GPCR) pathways, influence tumor progression, but their expression profiles, genomic alterations, immune associations, and prognostic roles in BC remain incompletely understood. This study systematically investigated the RGS family to identify potential biomarkers and therapeutic targets. - Source: PubMed
Mirzaei ZohrehMoghaddam Madiheh MazaheriBarati TaherehEbrahimi AmirKhaniani Mahmoud Shekari - Alzheimer's disease (AD) is characterized by progressive neurodegeneration in regionally vulnerable brain areas, yet molecular insights into early pathogenic mechanisms remain limited. - Source: PubMed
Publication date: 2026/06/01
Jaberi Khojaste RahimiAlashti Shayan KhaliliHooshmandi SedigheVatankhah PooyaHaghighi Maryam RohaniSavardashtaki AmirAligholi HadiJaberi Abbas Rahimi