MIXL1 antibody - N-terminal region (ARP30077_P050)
- Known as:
- MIXL1 (anti-) - N-terminal region (ARP30077_P050)
- Catalog number:
- arp30077_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- MIXL1 antibody - N-terminal region (ARP30077_P050)
Related genes to: MIXL1 antibody - N-terminal region (ARP30077_P050)
- Gene:
- MIXL1 NIH gene
- Name:
- Mix paired-like homeobox
- Previous symbol:
- -
- Synonyms:
- MILD1, MIXL
- Chromosome:
- 1q42.12
- Locus Type:
- gene with protein product
- Date approved:
- 2002-11-11
- Date modifiied:
- 2014-11-19
Related products to: MIXL1 antibody - N-terminal region (ARP30077_P050)
Related articles to: MIXL1 antibody - N-terminal region (ARP30077_P050)
- Perturbation studies using gene knockouts have become a key tool for understanding the roles of regulatory genes in development. However, large-scale studies dissecting the molecular role of development master regulators in every cell type throughout the embryo are technically challenging and scarce. Here, we systematically characterize the knockout effects of the key developmental regulators rachyury and in gastrulation and early organogenesis using single-cell profiling of chimeric mouse embryos. For the analysis of these experimental data, we present COSICC, an effective suite of statistical tools to characterize perturbation effects in complex developing cell populations. We gain insights into 's role in lateral plate mesoderm, limb development, and posterior intermediate mesoderm specification. Furthermore, we generate embryonic chimeras and reveal the role of this key transcription factor in discrete mesoderm lineages, in particular concerning developmental dysregulation of the recently identified juxta-cardiac field. - Source: PubMed
Publication date: 2025/04/10
Strauss Magdalena ETon Mai-Linh NuMason SamanthaBagri JaanaHarland Luke T GImaz-Rosshandler IvanWilson Nicola KNichols JenniferTyser Richard C VGöttgens BertholdMarioni John CGuibentif Carolina - Human induced pluripotent stem cells (hiPSCs) possess the ability to differentiate into a multitude of cell and tissue types but display heterogeneous propensity of differentiation into specific lineage. Characterization of the transcriptome of 11 hiPSC lines showed that activation of MIXL1 at the early stage of stem cell differentiation correlated with higher efficacy in generating definitive endoderm and advancing differentiation and maturation of endoderm derivatives. Enforced expression of MIXL1 in the endoderm-inefficient hiPSCs enhanced the propensity of endoderm differentiation, suggesting that modulation of key drivers of lineage differentiation can re-wire hiPSC to the desired lineage propensity to generate the requisite stem cell products. - Source: PubMed
Publication date: 2025/04/24
Osteil PierreWithey SarahSantucci NicoleAryamanesh NaderPang IgnatiusSalehin NazmusSun JaneQin AnnieSu JiayiKnowles HilaryLi Xiucheng BellaCai SimonWolvetang ErnstTam Patrick P L - A population of putative mesendoderm progenitors that can contribute cellular descendants to both mesoderm and endoderm lineages is identified in the gastrulating mouse embryo. These progenitor cells are localized to the posterior epiblast, primitive streak, and nascent mesoderm of mid-streak- (E7.0) to late-streak-stage (E7.5) embryos. Lineage tracing in vivo identified that putative mesendoderm progenitors contribute descendants to the definitive endoderm and the axial mesendoderm of E7.75 embryos and to the endoderm of the foregut and hindgut of the E8.5-8.75 embryos. Differentiation of mouse epiblast stem cells identified that the choice between endoderm and mesoderm cell fates depends on the timing of Mixl1 activation upon exit from pluripotency. The knowledge gained on the spatiotemporal distribution of mesendoderm progenitors and the molecular drivers underpinning the divergence of cell lineages in these progenitors enriches our mechanistic understanding of the allocation of the tissue progenitors to germ layer derivatives in early development. - Source: PubMed
Publication date: 2025/03/24
Masamsetti V PragathiSalehin NazmusKim Hani JieunSantucci NicoleWeatherstone MeganMcMahon RileyMarshall Lee LKnowles HilarySun JaneStuddert Josh BAryamanesh NaderWang RanJing NaiheYang PengyiOsteil PierreTam Patrick P L - Several differentiation protocols have enabled the generation of intermediate mesoderm (IM)-derived cells from human pluripotent stem cells (hPSC). However, the substantial variability between existing protocols for generating IM cells compromises their efficiency, reproducibility, and overall success, potentially hindering the utility of urogenital system organoids. Here, we examined the role of high levels of Nodal signaling and BMP activity, as well as WNT signaling in the specification of IM cells derived from a UCSD167i-99-1 human induced pluripotent stem cells (hiPSC) line. We demonstrate that precise modulation of WNT and BMP signaling significantly enhances IM differentiation efficiency. Treatment of hPSC with 3 μM CHIR99021 induced TBXT+/MIXL1+ mesoderm progenitor (MP) cells after 48 h of differentiation. Further treatment with a combination of 3 μM CHIR99021 and 4 ng/mL BMP4 resulted in the generation of OSR1+/GATA3+/PAX2+ IM cells within a subsequent 48 h period. Molecular characterization of differentiated cells was confirmed through immunofluorescence staining and RTqPCR. Hence, this study establishes a consistent and reproducible protocol for differentiating hiPSC into IM cells that faithfully recapitulates the molecular signatures of IM development. This protocol holds promise for improving the success of protocols designed to generate urogenital system organoids , with potential applications in regenerative medicine, drug discovery, and disease modeling. - Source: PubMed
Publication date: 2024/06/03
Magro-Lopez EsmeraldaVazquez-Alejo ElenaEspinar-Buitrago María de la SierraMuñoz-Fernández María Ángeles - Hepatoblastoma (HB) is the most common liver cancer in children, posing a serious threat to children's health. Chemoresistance is the leading cause of mortality in patients with HB. A more explicit definition of the features of chemotherapy resistance in HB represents a fundamental urgent need. - Source: PubMed
Publication date: 2023/07/17
Xiang XiaoHao YijieCheng ChengHu HuanjingChen HuadongTan JiehuiWang YuanqiLiu XiaofeiPeng BoLiao JunbinWang JiXie YubinLiu JunchengChen ShulingXu LixiaXie WenxuanXue RuidongKuang MingXu ZheJiang HongPeng Sui