EEA1 antibody - N-terminal region (ARP30074_P050)
- Known as:
- EEA1 (anti-) - N-terminal region (ARP30074_P050)
- Catalog number:
- arp30074_p050
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- EEA1 antibody - N-terminal region (ARP30074_P050)
Related genes to: EEA1 antibody - N-terminal region (ARP30074_P050)
- Gene:
- EEA1 NIH gene
- Name:
- early endosome antigen 1
- Previous symbol:
- -
- Synonyms:
- ZFYVE2
- Chromosome:
- 12q22
- Locus Type:
- gene with protein product
- Date approved:
- 1998-12-23
- Date modifiied:
- 2016-11-09
Related products to: EEA1 antibody - N-terminal region (ARP30074_P050)
Related articles to: EEA1 antibody - N-terminal region (ARP30074_P050)
- Chen's Jinshui Pills (CJSP), a traditional Chinese medicinal formula, is commonly used to treat ophthalmic disorders. However, its therapeutic effects and mechanisms in diabetic retinopathy (DR) require further validation and exploration. - Source: PubMed
Publication date: 2026/04/07
Jiang YangDing JiazhenZhou ManyuYang XiangzhuLiang YaoZhao XinweiWang JingxuanLiao Yan - The irreversible inflammation associated with cariogenic pulp injury presents a major challenge in clinical dentistry, as current pulp-capping materials are unable to precisely regulate the early activation of the complement system. To address this limitation, the present study successfully constructed a bisphenol A glycerolate dimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA), and 10-(methacryloyloxy)decyl dihydrogen phosphate (10-MDP)-based adhesive mixture (BTHM) loaded with the endocytosis inhibitor Dynasore (Dynasore@BTHM). Material characterization (FT-IR, XPS, EDS, DMA, and SEM) confirmed that Dynasore is dispersed within the hydrophobic Bis-GMA/TEGDMA network. Based on these observations, we propose that hydrogen bonding with HEMA enables stable drug loading and may contribute to directional enrichment at the dentin-pulp interface, leading to controlled sustained release. Performance evaluation demonstrated that 2 wt % Dynasore@BTHM exhibits excellent sustained-release characteristics while maintaining favorable mechanical strength, marginal sealing, and biocompatibility. In vitro studies verified that this system inhibits dynamin-mediated endocytosis of C5a/C5aR, effectively down-regulating the expression of EEA-1, C5aR, and the pro-inflammatory cytokine IL-6. Alizarin red staining further indicated its ability to promote pulp cell mineralization. Moreover, in vivo experiments showed that 2 wt % Dynasore@BTHM effectively alleviated inflammatory cell infiltration in pulp tissue and downregulated the expression of key inflammatory factors. Collectively, this study provides a strategy for developing pulp repair materials integrating adhesive function and immunomodulatory capability. By targeting the endocytic pathway, it enables precise and coordinated regulation of the inflammation-regeneration process, thereby exhibiting broad application prospects in the early treatment of cariogenic pulp injury. - Source: PubMed
Publication date: 2026/04/01
Chen YanHan ZhongqiangZhang YueZhai DongxueYang WenZheng ShuaiChen ZhuangyingWang TongfangLiu ChangweiLiu Mingyue - Glutamate transporters are essential for maintaining CNS homeostasis by clearing extracellular glutamate after synaptic transmission. Dysregulation of these transporters contributes to excitotoxicity in numerous neurological disorders, including ischemic stroke, making them a promising therapeutic target. However, the regulatory response of these transporters following ischemic insult remains poorly defined. In this work, using a model of oxygen-glucose deprivation in primary glia cultures, we report aberrant trafficking of the astrocytic glutamate transporter GLT-1 following ischemic insult. This response is characterized by increased transporter internalization and degradation, accompanied by reduced glutamate uptake capacity. Focusing on post-translational modifications (PTMs), we found that GLT-1 ubiquitination is markedly increased after ischemic insult and contributes to transporter internalization. Importantly, disrupting this ubiquitination interaction through mutation of C-terminal GLT-1 lysine residues restores GLT-1 surface expression and rescues glutamate uptake capacity through preventing early endosome 1 (EEA1)-mediated internalization. Additionally, we report that inhibition of C-terminal GLT-1 PTMs confers neuroprotection following ischemic insult in organotypic rat brain slices. Together, these findings demonstrate that ischemia-induced dysregulation of GLT-1 trafficking plays a critical role in impaired glutamate clearance and cellular recovery, highlighting GLT-1 ubiquitination as a potential therapeutic target for ischemic injury. - Source: PubMed
Publication date: 2026/03/17
Gill Simran KaurReeb Katelyn LouiseKroll MaxMortensen Ole VFontana Andréia Cristina Karklin - Cardiovascular diseases are a major global health burden, demanding phenotyping frameworks that can match the scale and complexity of contemporary mouse genetics. Here, we introduce EchoVisuALL, an AI-enabled pipeline for automated high-throughput transthoracic echocardiography (TTE) coupling deep-learning-based left-ventricular segmentation with data reporting. Across 65,000 recordings from over 18,000 mice, including single-gene knockouts from the International Mouse Phenotyping Consortium, the framework quantified cardiac morphology and function with minimal operator dependency and high reliability, validated against an expert-curated gold standard dataset. By extracting quantitative parameters across the cardiac cycle, EchoVisuALL in combination with multi-dimensional clustering uncovered nonlinear phenotypic relationships and revealed 37 of 715 genes associated with significant cardiac abnormalities, encompassing well-known human disease genes as well as 12 previously unrecognized candidates, including , , , , , and . These genotype-phenotype associations are involved in myocardial energetics, membrane biology, and cardiac remodeling. We demonstrate the potential of EchoVisuALL to move beyond image segmentation by delivering a standardized, quantitative foundation for scalable downstream analyses, enabling the discovery of novel cardiac disease genes. - Source: PubMed
Publication date: 2026/02/19
Galter IsabellaSchneltzer ElidaMarr CarstenSpielmann NadineHrabě de Angelis Martin - The combination of supported lipid bilayers (SLBs) with the Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) has been proven to be a powerful tool to simultaneously monitor mass and viscoelastic changes related to membrane binding-events. In this work, the above methodology is employed for the study of the interaction of the Early Endosomal Antigen 1 (EEA1) to a model lipid bilayer that mimics the early endosome (EE) membrane, focusing on the membrane composition. Starting with the formation of a lipid bilayer through the vesicles fusion technique, we investigated the formation of SLBs that incorporate phosphatidylinositol 3-phosphate (PI(3)P), a key component for EEA1 binding, in combination with other lipids, e.g., (1,2-dioleoyl-sn-glycero-3)-phosphocholine (DOPC), -phosphoserine (DOPS), -phosphoethanolamine (DOPE), and cholesterol (Chol). The interaction of the full-length coiled-coil EEA1 to the formed SLBs was further studied in real time with the QCM-D and characterized with respect to the lipid composition and pH. Our findings confirm that PI(3)P is essential for the EEA1-membrane interaction, while it was shown that Chol and phosphatidylserine greatly influence the binding event. In fact, including 30% Chol in a PI(3)P (3%):PS (6%) SLB resulted in almost double EEA1 binding than in the absence of Chol. Moreover, we employed the QCM-viscoelastic model available to analyze the QCM-D data with emphasis on the study of the protein conformation. Our results showed that, in our in vitro system, EEA1 is not fully extended and/or highly packed, but is mainly in a bent, distorted conformation with an average size close to 100 nm. This study complements previous works employing in vitro assays, also demonstrating the ability to reconstitute more complex biomimetic EE membranes containing inositol phospholipids on a QCM surface for the study of EEA1 binding. - Source: PubMed
Publication date: 2026/01/26
Papagavriil FotiniMateos-Gil PabloLauer JanelleZerial MarinoGizeli Electra