P4HB Antibody (AMM10025)
- Known as:
- P4HB Antibody (AMM10025)
- Catalog number:
- amm10025
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- P4HB Antibody (AMM10025)
Related genes to: P4HB Antibody (AMM10025)
- Gene:
- P4HB NIH gene
- Name:
- prolyl 4-hydroxylase subunit beta
- Previous symbol:
- PO4DB, ERBA2L
- Synonyms:
- PDIA1, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta
- Chromosome:
- 17q25.3
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-10-05
Related products to: P4HB Antibody (AMM10025)
Related articles to: P4HB Antibody (AMM10025)
- Bladder cancer (BLCA) poses a significant clinical challenge due to its high mortality rates and the inadequacy of current prognostic biomarkers. Programmed cell death (PCD) is crucial in BLCA initiation, progression, and treatment, yet the interplay and specific roles of different PCD pathways in BLCA prognosis remain elusive. This study aimed to develop and validate predictive models by integrating 14 PCD patterns using comprehensive analyses of bulk RNA and single-cell RNA transcriptomic data from TCGA-BLCA and six GEO datasets. Through weighted gene co-expression network (WGCNA) analyses, 24 hub PCD-related genes (PCDGs) were identified in BLCA. Subsequently, we implemented a computational framework that integrated 10 machine learning algorithms along with 101 of their combined permutations. This framework was used to develop a programmed cell death-related signature (PCDRS). The final PCDRS consisted of 12 prognostic genes: P4HB, CHEK2, PTPN2, ATP13A2, CCT6A, TFRC, RRP12, TRAF7, POLR1B, B4GALT3, SIVA1, and TP73.The PCDRS was validated in training and external validation sets, with multivariate analysis confirming its independent prognostic value in BLCA. The PCDRS-integrated nomogram was also developed as a quantitative clinical tool. Furthermore, differences in reactive oxygen species (ROS) levels were observed in the tumor microenvironment between high- and low-risk groups based on PCDRS risk scores. Additionally, the elevated expression and tumorigenic role of P4HB in BLCA were validated through in vitro assays. In summary, P4HB may serve as a candidate gene with potential relevance to BLCA prognosis that could enhance personalized treatment strategies for patients with BLCA. - Source: PubMed
Publication date: 2026/04/02
Cao YangLi CanHua YiboWu TingtingShen QiuyuLin ZeyuHuang Yuhua - : The extracellular matrix (ECM) plays a central role in the mechanical strength and functional integration of tissue-engineered matrix (TEM), particularly in cardiovascular and load-bearing applications. Mesenchymal stromal cells (MSCs) from different sources may vary in their ECM-forming potential. : In this study, adipose-derived (hADMSC), bone marrow-derived (hBMSC), and umbilical cord-derived MSCs (hUCMSC) were compared with human dermal fibroblasts (HDFBs) as a reference. Cells were seeded onto polyglycolic acid (PGA)/poly-4-hydroxybutyrate (P4HB) scaffolds and cultured for 3 weeks under static or hydrodynamic conditions using orbital shaking. TEM development was assessed macroscopically, histologically (using H&E and Masson's trichrome stains), and by polarized light microscopy (Picrosirius Red), alongside biochemical assays that quantified DNA, glycosaminoglycan (GAGs), and hydroxyproline (HYP). : Hydrodynamically stimulated culture consistently improved ECM deposition across all groups. TEMs exposed to hydrodynamic stimulation (hydrodynamic conditions) were thicker, more uniformly filled, and exhibited increased collagen deposition compared with static TEMs, which remained thinner and showed persistent scaffold remnants. Polarized light analysis demonstrated that dynamic loading promoted collagen maturation in all groups, as evidenced by an increased prevalence of thick, birefringent collagen fibers indicative of mature collagen. Biochemical analyses showed that HDFB-derived TEMs produced the highest total collagen and ECM content under both static and hydrodynamic conditions; however, these matrices remained comparatively thin and densely packed. In contrast, MSC-derived TEMs formed thicker and more spatially distributed ECM in response to hydrodynamic stimulation. : Among the MSC sources, hUCDMSC-derived TEMs exhibited the most advanced collagen maturation and the most uniform collagen distribution under hydrodynamically stimulated culture, whereas hADMSC-derived TEMs showed the greatest matrix thickening and volumetric ECM expansion with intermediate collagen maturation. hBMSC-derived TEMs displayed clear responsiveness to hydrodynamic stimulation but remained limited in overall collagen deposition and fiber maturation. These findings underscore that both hydrodynamic stimulation and cell source are critical not only for maximizing ECM deposition, but also for ensuring physiologically relevant collagen maturation and matrix organization in grafts suitable for clinical translation. - Source: PubMed
Publication date: 2026/02/28
Klein MichelleEhterami ArianRanjbar NeguinHoerstrup Simon PEmmert Maximilian YGenerali Melanie - Revision breast reconstruction is often necessary to address complications, reduce asymmetry, or improve the aesthetic result. Our previously described use of poly-4-hydroxybutyrate (P4HB) has shown promise in primary reconstruction; limited data exist on its use in revision surgery. This study presented a single-institution case series evaluating indications and outcomes of revision breast reconstruction using a P4HB-wrapped implant. - Source: PubMed
Publication date: 2026/03/19
Sorenson Thomas JBoyd Carter JHemal KshipraCohen OrianaChoi MihyeKarp Nolan - Endoplasmic reticulum stress (ERS) and apoptosis are hallmark pathological features of myocardial ischemia-reperfusion injury (MIRI). Lamin A/C, a nuclear lamina protein associated with cardiac disorders, has been implicated in ERS and apoptosis regulation, yet its role in MIRI remains elusive. Meanwhile, P4HB, an ERS-associated chaperone, may interact with lamin A/C to modulate MIRI progression. We hypothesized that lamin A/C interacts with P4HB to modulate MIRI progression. We assessed the distribution of P4HB and lamin A/C in MIRI SD rat hearts and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated primary neonatal rat cardiomyocytes (PNRCMs). By knocking down lamin A/C expression, we examined the impact of lamin A/C on P4HB distribution, ERS and apoptosis induced by OGD/R modeling. STRING network analysis and protein-protein docking were employed to predict the structural basis of lamin A/C/P4HB interaction. Our results demonstrated OGD/R treatment triggered P4HB nuclear envelope translocation, ERS activation, and apoptosis in PNRCMs, while those effects were attenuated by lamin A/C knockdown. The physical interaction between lamin A/C and P4HB in cardiac tissue was observed under both normal and MIRI conditions. The P4HB-lamin A/C interaction may be dynamically modulated by calreticulin. Collectively, our findings propose that lamin A/C regulates P4HB to mitigate OGD/R-induced ERS and apoptosis, potentially through a calreticulin-mediated dynamic mechanism. - Source: PubMed
Publication date: 2026/03/19
Zhou JiahuiLi XinzhongLi NaWang MinSun Guibo - Fully resorbable biosynthetic mesh composed of poly-4-hydroxybutyrate (P4HB), have been designed for incisional hernia (IH) repair, including in contaminated surgical fields. While existing studies have demonstrated its safety and efficacy in the short term, comprehensive long-term data, particularly after complete mesh resorption, remain scarce. - Source: PubMed
Publication date: 2026/03/20
van den Berg RudolfWieser MarieLópez-Cano ManuelBueno-Lledó JoséKöckerling FerdinandChatzimavroudis GrigoriosGonella-Pacchiotti ConstanzaStabilini CesareOrtega-Deballon PabloRomain Benoit