RPS6 (phospho-Ser235) Antibody
- Known as:
- RPS6 (phosphorilated-Ser235) Antibody
- Catalog number:
- abx000279
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- RPS6 (phospho-Ser235) Antibody
Related genes to: RPS6 (phospho-Ser235) Antibody
- Gene:
- RPS6 NIH gene
- Name:
- ribosomal protein S6
- Previous symbol:
- -
- Synonyms:
- S6
- Chromosome:
- 9p22.1
- Locus Type:
- gene with protein product
- Date approved:
- 1990-07-06
- Date modifiied:
- 2016-10-05
Related products to: RPS6 (phospho-Ser235) Antibody
Related articles to: RPS6 (phospho-Ser235) Antibody
- Hepatocellular carcinoma (HCC) remains a lethal malignancy with limited therapeutic options. Kaempferol shows potential in suppressing HCC progression via glycolysis regulation, yet its molecular targets and mechanisms are unclear. Transcriptomic data were analyzed to identify HCC-related DEGs. Intersection with kaempferol's glycolysis-related targets yielded candidate genes. A prognostic risk model was constructed and validated by ROC curves and survival analysis. GSEA and single-cell RNA sequencing explored mechanisms and cellular heterogeneity. Mendelian randomization assessed causality between prognostic genes and HCC. After HCC cells were treated with different concentrations of kaempferol, the effects of kaempferol on proliferation, invasion, and migration of HCC cells were detected by CCK-8, cell clone formation, and Transwell cell scratch healing experiments. The expression levels of key proteins in the AKT-mTOR signaling pathway and glycolytic rate-limiting enzymes were detected by Western blot. The spectrophotometric method was used to detect the effect of kaempferol on glucose uptake and lactate production of HCC cells. Finally, the impact of knocking down CA9 on glucose uptake and lactic acid production in liver cancer cells was analyzed. The prognostic risk model identified GRK6, ABCC1, CA9, and CDK5R1 as prognostic genes. GSEA implicated cell cycle and PI3K-Akt pathways in HCC progression driven by these genes. Single-cell analysis revealed pronounced upregulation of prognostic genes in hepatocytes. Finally, MR analysis confirmed that CDK5R1 was a risk factor for the incidence of HCC. Kaempferol significantly inhibited the proliferation, invasion, and migration of MHCC97H and Huh7 cells, as well as the expression levels of PKM2, HK2, p-AKT, p-mTOR, and p-RPS6. Kaempferol significantly reduced glucose uptake and lactic acid production in MHCC97H and Huh7 cells. Knockdown of CA9 inhibited the uptake of glucose and lactic acid production, along with suppressing the expression of HK2 and PKM2 in MHCC97H and Huh7 cells. This study found that kaempferol targeted the AKT-mTOR pathway and downregulated the CA9 gene, thereby modulating glycolysis and ultimately suppressing the proliferation, invasion, and migration of hepatocellular carcinoma. - Source: PubMed
Xu HanQian LeCao YajieOu MingruiNi YuZhang BiyingChen LingruHao JiqingWang Zishu - parasitizes the Asian citrus psyllid (ACP), , the primary insect vector responsible for transmitting Huanglongbing (HLB), a severe citrus disease. Screening of appropriate reference genes is a critical prerequisite for reliable RT-qPCR analysis, which is essential for investigating the functions of target genes in across diverse experimental conditions. However, to date, no validated reference genes have been reported for this species. This study assessed seven housekeeping genes in under six conditions (developmental stage, body tissue, population, temperature, diet, and starvation) using five stability algorithms (, , , , and ∆). The results identified the most suitable reference genes for specific experimental conditions: and for the developmental stage; and for population comparisons; and for different feeding diets; and for starvation; and for different body tissues (head, thorax, abdomen) and temperature gradients (5 °C, 15 °C, 25 °C, 35 °C). Furthermore, the expression profiles of were markedly different when normalized to the most versus the least stable reference genes across body tissues, diets, starvation durations, and temperatures. Our findings establish the first set of RT-qPCR reference genes for , providing a useful foundation for functional genomics research on this biological control agent. - Source: PubMed
Publication date: 2026/05/30
Gu XiaohangLuo BingruiZhang SiyiChen JialiangXu PeipingLi ShuangQiu BaoliZhang Lihe - Acute-on-chronic liver failure (ACLF) is characterized by profound metabolic dysfunction and high mortality. Identifying amino acid metabolism-related biomarkers is crucial for early diagnosis and therapeutic intervention. Amino acid metabolism-related genes (AAMGs) and ACLF transcriptomic data were integrated to identify core genes via weighted gene co-expression network analysis (WGCNA) and differential expression analysis. Three machine learning (ML) algorithms-Random Forest, SVM-RFE, and Boruta-were applied to screen hub genes. Specific cellular profiles and immune landscapes were characterized using single-cell RNA sequencing (scRNA-seq) and ssGSEA. The pathological role of BCAT1 was investigated in APAP-induced ACLF models. A total of 26 genes were identified at the intersection of module genes, DEGs, and AAMGs. ML confirmed a three-gene signature (BCAT1, RPS6, OAT) with high diagnostic accuracy. ScRNA-seq analysis further verified that BCAT1 is predominantly expressed in hepatocytes, indicating its cell-type specific role in liver injury progression. In vitro and in vivo experiments demonstrated that BCAT1 exacerbates hepatocyte injury by activating the GSDMD/Caspase-1-mediated pyroptosis pathway. Our study reveals that BCAT1 is a pivotal driver of hepatocyte pyroptosis in ACLF. Targeting the BCAT1-mediated metabolic-inflammatory axis offers a promising therapeutic strategy for managing ACLF. - Source: PubMed
Li MengyueLu BinChen XingzhiKang ManqiuWang GangWang LifengYe HuowangPu FulinWang MeiqianWen WeihengChen Yiru - Chromatin stability is essential for maintaining genome integrity and gene regulation in eukaryotic cells. Nucleosome assembly protein 1 (Nap1) is a key regulator of chromatin dynamics across different organisms. The ciliate Tetrahymena thermophila contains two functionally distinct nuclei within a single cell: the diploid germline micronucleus (MIC), which undergoes mitosis and meiosis, and the polyploid somatic macronucleus (MAC), which divides amitotically. However, the function of Nap1 in this evolutionarily distant protist remains unclear. Here, we show that Nap1 localizes predominantly to the perinuclear region of the MAC during vegetative growth and is also detectable in the MAC, MIC, and cytoplasm. Truncation of the nuclear export signal (NES) increases accumulation of Nap1 in the MAC. During sexual development, Nap1 localizes to the cytoplasm, parental MAC, and developing new MAC, while Nap1 shows enhanced enrichment in the new MAC. Nap1 also partially colocalizes with the nuclear pore protein Nup98 at the MAC envelope. Knockdown of NAP1 impairs proliferation, disrupts amitotic MAC division, and induces nuclear extrusion bodies. Moreover, NAP1 deficiency causes abnormal meiotic progression. Co-immunoprecipitation coupled with mass spectrometry showed that Nap1 associates with nuclear pore complex proteins, histones, and DNA replication/repair factors. Direct binding of Nap1 to H2A-H2B and the ribosomal protein Rps6 was confirmed by pull-down assays. These findings establish Nap1 as a multifunctional protein required for nuclear envelope integrity, nuclear division, and chromatin stability in Tetrahymena. - Source: PubMed
Publication date: 2026/06/05
Yang SitongMi JinXu JingWang Wei - Platinum-based chemotherapy for ovarian cancer is frequently compromised by the development of drug resistance, which is often accompanied by an immunosuppressive tumor microenvironment. The molecular mechanisms linking cisplatin resistance to immune evasion remain poorly understood, hindering the development of effective combination therapies. - Source: PubMed
Publication date: 2026/06/04
Lv FeiZhao QihangZou RuoyaoYe XiaoyeShen YidanMeng LijunGuo JialuWang YahuiZheng YinglingLiu YanpengWang ShuaiWu YongfengTong Jinyi