GSK3A (phospho-Ser21) Antibody
- Known as:
- GSK3A (phosphorilated-Ser21) Antibody
- Catalog number:
- abx000172
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- GSK3A (phospho-Ser21) Antibody
Related genes to: GSK3A (phospho-Ser21) Antibody
- Gene:
- GSK3A NIH gene
- Name:
- glycogen synthase kinase 3 alpha
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 19q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1997-05-15
- Date modifiied:
- 2016-10-05
Related products to: GSK3A (phospho-Ser21) Antibody
Related articles to: GSK3A (phospho-Ser21) Antibody
- Idiopathic male infertility is a rising global concern characterised by ejaculatory defects, absence or low sperm count with abnormal morphology, and poor sperm motility. Deciphering the aetiology of male infertility requires a fundamental understanding of multiple spermatogenic events, including sperm maturation. Glycogen synthase kinase 3 paralog-α (GSK3α) plays a critical role in sperm maturation, specifically during epididymal motility, capacitation and hyperactivation. Methylation modulates mRNA transport, stability, turnover, and translational efficiency to meet cellular requirements. The mRNA demethylase, fat mass and obesity-associated protein (FTO), is a target for phosphorylation by GSK3α, suggesting the potential role of this enzyme in male fertility. This study focuses on the high-affinity spatiotemporal interaction between FTO and GSK3α to delineate the post-transcriptional modifications in the murine testis. Expression of Gsk3a and Fto increases temporally starting with day 18-20 postpartum testis, coinciding with meiosis I and gradually peaks by day 25-34 with the formation of spermatids and completion of spermatogenesis. Co-immunoprecipitation of GSK3α and GSK3β with FTO using respective antibodies and super-resolution microscopy shows a preferential interaction of GSK3α with FTO. Moreover, Gsk3a knockout mice showed significantly low m6A levels in testis, presumably due to enhanced FTO activity. In silico protein-protein docking and molecular dynamics analysis demonstrated an energetically favourable, consensus phosphorylation motif-dependent high-affinity interaction between FTO and GSK3α, further validating our observation. A specific missense mutation (Cys326 > Ser) permitted an additional GSK3-phosphorylation site in FTO, leading to teratozoospermia in a patient. Collectively, this study affirms GSK3α as a spatiotemporal regulator of FTO function inside the mammalian testis. - Source: PubMed
Publication date: 2026/08/10
Choudhari NehaDehury BudheswarRoy RounakPeriyasamy RadhakrishnanBhattacharya IndrashisSahare MaheshVijayraghavan SrinivasanDey Souvik - Despite significant advancements in CRISPR/Cas-based genome editing technology over the past decade, achieving simultaneous homozygous gene editing at multiple targets in primary cells remains a major challenge. In this study, we developed and constructed a CRISPR multi-gene targeting system that integrates episomal vectors with tRNA-sgRNA array technology. This approach leverages scaffold/matrix attachment region (S/MAR) sequences to enable sustained episomal expression of both Cas9 and single-guide RNAs (sgRNAs) without genomic integration, thereby enhancing gene editing efficiency. For simultaneous editing of multiple loci, we used the tRNA-sgRNA architecture to process multiple sgRNAs from a single vector. Using this system in porcine fetal fibroblasts, we achieved concurrent editing of six genes, namely , , , , , and , in individual cells. These edited cells supported normal development following somatic cell nuclear transfer, yielding blastocysts with unaltered developmental competence. Collectively, our findings establish a framework for the application of CRISPR/Cas9 in gene-edited pigs, facilitating the generation of multi-gene-edited animals for biomedical and agricultural applications. - Source: PubMed
Publication date: 2026/05/08
Jiang ChaoqianYang DongyanSun ChengboRen XingruiLi TianzeWu JiayanTian JianFeng MingjieYao YuchangSong JunWeng XiaogangMu Yanshuang - Atherosclerosis (AS) is a leading cardiovascular disease driven by lipid metabolism dysregulation and immune maladaptation. Although macrophage autophagy modulates plaque stability, the specific autophagy-related genes governing AS progression, particularly in spatial and immune contexts, remain poorly defined. This study aimed to systematically identify and characterize key macrophage autophagy-associated genes in AS using an integrated multi-omics approach. - Source: PubMed
Publication date: 2026/02/26
Cao WenqiWang BingyangWang YuxueLi YunyanYang HanningSun YueXu LirongLu Yongping - Organoids derived from pluripotent stem cells have emerged as powerful models to study human development. To investigate signaling pathways regulating human pancreas differentiation and morphogenesis, we developed a high-content, image-based screen and quantitative multivariate analysis pipelines robust to heterogeneity to extract single-cell and organoid features using pancreatic progenitor organoids. Here, we identified 54 compounds affecting cell identity and/or morphological landscape. Focusing on one family of compounds, we found that glycogen synthase kinase 3α/β (GSK3A/B) inhibition via wingless/int-1 (WNT) signaling has a reversible effect on cell identity, repressing pancreatic progenitor markers and inducing a poised state in progenitors transitioning to acinar cells. We show that additional fibroblast growth factor (FGF) repression enables further differentiation of acinar cells, recapitulating pancreatic acinar morphogenesis and function. The ability to produce acinar cells is valuable for future studies on pancreatic exocrine function and cancer initiation in humans, as acinar cells are thought to be an important cell of origin for pancreatic adenocarcinoma. - Source: PubMed
Publication date: 2026/01/21
Keshara RashmiparvathiKuodyte KarolinaJanosch AntjeAndree CordulaBickle MarcStöter MartinBarsacchi RicoKim Yung HaeGrapin-Botton Anne - Right ventricular dysfunction (RVD) is a common complication of idiopathic dilated cardiomyopathy linked to poor outcomes. However, reliable noninvasive biomarkers for RVD remain lacking. This study aimed to identify urinary proteomic markers using mass spectrometry and machine learning. - Source: PubMed
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